Helicobacter pylori Selective Medium Base | Selective Isolation Medium | AS‑1249
$55.00 AUD
AuSaMicS Life Science • Clinical Microbiology Media
Helicobacter pylori Selective Medium Base
Selective Base for Recovery of H. pylori from Gastric Biopsy Specimens
A rich selective agar base formulated for the isolation, recovery, and primary cultivation of Helicobacter pylori from gastric biopsy and related clinical samples. When prepared with sterile defibrinated blood and an appropriate Dent/VTCA-type selective supplement, the medium supports slow-growing microaerophilic H. pylori while suppressing common bacterial and fungal contaminants.
AS-1249 pH 7.3 ± 0.2 ✓ Australian Stock 🧫 Microaerophilic Recovery 🩸 Blood-Enriched Use
🔬 Application: Gastric biopsy culture 🌫️ Atmosphere: Microaerophilic incubation 📋 Documents: COA + SDS + TDS available
Optimised for Difficult Recovery
Used with blood for fastidious recovery
Selective system reduces background flora
Microaerophilic incubation compatible
Clinical microbiology workflow ready
Consistent batch quality from AuSaMicS
Typical Use Format
Base medium
+ sterile blood
+ Dent/VTCA-type supplement
= Selective H. pylori isolation medium
+ sterile blood
+ Dent/VTCA-type supplement
= Selective H. pylori isolation medium
⚠️ Important: This is a Medium Base — Not the Complete Ready-to-Use Selective Agar
Helicobacter pylori Selective Medium Base (AS-1249)
Dehydrated nutrient agar base intended to be prepared with sterile blood and an appropriate Helicobacter selective supplement for optimal isolation of H. pylori.
Dehydrated nutrient agar base intended to be prepared with sterile blood and an appropriate Helicobacter selective supplement for optimal isolation of H. pylori.
Complete Selective Medium
The finished selective medium includes the base + blood + antimicrobial supplement. Without these additions, selectivity and recovery performance will not match the intended clinical workflow.
The finished selective medium includes the base + blood + antimicrobial supplement. Without these additions, selectivity and recovery performance will not match the intended clinical workflow.
🔬 Technical Overview
Helicobacter pylori is a slow-growing, fastidious, microaerophilic, curved Gram-negative bacterium associated with chronic gastritis, peptic ulcer disease, and other gastroduodenal disorders. Primary isolation from biopsy specimens is often challenging because the organism may be present in low numbers and can be overgrown by contaminating flora if a rich but selective medium is not used.
Helicobacter pylori Selective Medium Base is designed as a nutrient-rich solid support for this workflow. In routine use, the base is prepared with sterile defibrinated blood and a Dent- or VTCA-style selective supplement containing antimicrobial agents that inhibit Gram-positive bacteria, many Gram-negative contaminants, and yeasts or molds while allowing H. pylori to recover under microaerophilic conditions.
Why this medium format works:
The enriched agar base provides peptides, growth nutrients, and osmotic stability; blood contributes additional growth factors and improves recovery of stressed cells; selective supplement limits background organisms; and microaerophilic incubation provides the low-oxygen environment required for colony development.
The enriched agar base provides peptides, growth nutrients, and osmotic stability; blood contributes additional growth factors and improves recovery of stressed cells; selective supplement limits background organisms; and microaerophilic incubation provides the low-oxygen environment required for colony development.
Typical colony appearance: small, translucent to greyish colonies may appear after prolonged incubation, commonly within 3–7 days depending on specimen quality, inoculum, and incubation conditions.
🧪 Typical Medium System Components
🧫 Typical Isolation Workflow
1
Prepare enriched selective medium
Prepare the base according to instructions, cool to suitable pouring temperature, then aseptically add sterile blood and the selected Helicobacter supplement.
Prepare the base according to instructions, cool to suitable pouring temperature, then aseptically add sterile blood and the selected Helicobacter supplement.
2
Inoculate clinical specimen
Streak gastric biopsy or related specimen promptly to minimise viability loss. Fresh biopsy material generally offers the best isolation yield.
Streak gastric biopsy or related specimen promptly to minimise viability loss. Fresh biopsy material generally offers the best isolation yield.
3
Incubate microaerophilically
Incubate plates under microaerophilic conditions, typically around 35–37°C, for several days. Slow colony development is expected.
Incubate plates under microaerophilic conditions, typically around 35–37°C, for several days. Slow colony development is expected.
4
Confirm presumptive colonies
Suspect colonies should be confirmed by standard laboratory procedures such as Gram stain, oxidase, catalase, urease, and other appropriate identification methods.
Suspect colonies should be confirmed by standard laboratory procedures such as Gram stain, oxidase, catalase, urease, and other appropriate identification methods.
📊 Comparative Media for Helicobacter Recovery
📌 Quick Specifications
Product: Helicobacter pylori Selective Medium Base
Cat. No.: AS-1249
Format: Dehydrated culture medium base
Target organism: Helicobacter pylori
Typical specimen: Gastric biopsy
Supplement requirement: Yes
Blood enrichment: Required / recommended
Storage: 10–25°C, dry, tightly closed, protected from moisture and light
⭐ Key Advantages
✓Designed for demanding H. pylori isolation workflows
✓Supports fastidious organism recovery with blood enrichment
✓Compatible with established Dent/VTCA-style supplement systems
✓Useful where background flora would overwhelm non-selective media
✓Suitable for hospital, pathology, academic, and research laboratories
⚙️ Preparation Notes
Prepare the dehydrated base according to the product directions.
Cool to an appropriate temperature before aseptically adding sterile blood and the selected supplement.
Do not overheat after blood or antimicrobial supplement addition. Final preparation and incubation conditions should follow your validated laboratory method.
🔗 Related AuSaMicS Products
Columbia Blood Agar Base
General enriched base for fastidious organism recovery
Campylobacter / microaerophile media
Complementary options for fastidious curved Gram-negative organisms
Selective antibiotic supplements
Used to convert enriched bases into selective isolation media
Reliable Media for Demanding Clinical Recovery
AuSaMicS supplies high-quality microbiological media designed for dependable laboratory performance. For bulk orders, method alignment, or documentation requests for Helicobacter isolation workflows, contact our team.
For laboratory, research, and industrial use only. Not for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
www.ausamics.com.au • support@ausamics.com • +61 412 520 598