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Indole Nitrite Medium (AS-1250) | Trypticase Nitrate Broth
$95.00 AUD
AuSaMicS Life Science • Biochemical Differentiation
Indole Nitrite Medium
Trypticase Nitrate Broth for ID
A dual-purpose diagnostic medium used for the identification of microorganisms based on their ability to produce indole and reduce nitrate to nitrite. Highly effective for the differentiation of non-fermentative bacilli and Enterobacteriaceae.
AS-1250
⚗️ Dual Test Formula
✓ AOAC / BAM Compliant
🔬 High Clarity
Tests: Indole & Nitrate Reduction Incubation: 35°C / 18-48h Prep: 25.0g/L Yield
ID Specialist
Tryptophan Rich - Optimal for indole production
Standardized Nitrate - Reliable reduction assays
Broad Application - Clinically validated base
Clear Interpretation - Rapid color development
Differential Biochemistry
Pure Culture Identification
🧪 Biochemical Matrix
📋 Formulation (per Liter)
| Component | Concentration | Role |
|---|---|---|
| Pancreatic Digest of Casein | 20.0 g | High tryptophan source |
| Potassium Nitrate ($KNO_3$) | 1.0 g | Substrate for nitrate reduction |
| Dextrose | 1.0 g | Energy source for metabolism |
| Disodium Phosphate | 2.0 g | Buffering agent |
| Agar (Slight concentration) | 1.0 g | Improves anaerobic conditions |
Diagnostic Interpretation
Indole Test (+)
Formation of a Pink-Red ring at the surface after adding Kovacs' Reagent indicates tryptophanase activity.
Nitrate Reduction (+)
Development of a Red color after adding Sulfanilic acid and Naphthylamine confirms $NO_3$ to $NO_2$ reduction.
📋 Technical Profile
| Catalogue No. | AS-1250 |
| Final pH | 7.2 ± 0.2 |
| Yield | 25.0g / Liter |
| Appearance | Fine beige powder |
⚗️ Laboratory Prep
- 1 Suspend 25.0g in 1L deionized water.
- 2 Mix well and dispense into test tubes.
- 3 Autoclave at 121°C for 15 min.