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IPTG (Dioxane-Free) | ASE-901 | Lac Operon Inducer | AuSaMicS
$25.00 AUD
Documentation
Enzyme Substrates · Molecular Biology · lacZ Expression
IPTG (Dioxane-Free)
Isopropyl-β-D-thiogalactopyranoside · CAS 367-93-1
≥99.5% Purity
Dioxane-Free
Molecular Grade
Batch Traceable
AuSaMicS
Laboratory Division
Cat No.
ASE-901
CAS No.
367-93-1
Molecular Wt.
238.31 g/mol
HS Code
2932.99.00
Storage
-20°C, Dry
Description
IPTG (Isopropyl β-D-1-thiogalactopyranoside) is an essential synthetic molecular biology reagent utilized as a potent inducer of the lac operon in bacterial gene expression systems. Unlike lactose, IPTG is a non-metabolizable inducer; it is not hydrolyzed by β-galactosidase, providing stable and sustained induction of lac-controlled genes throughout the growth cycle.
Key Applications
Protein Expression
Induction of recombinant protein production in T7 and lac-based bacterial expression systems.
Cloning Selection
Critical component for blue-white colony screening (lacZ alpha-complementation) in molecular cloning.
Metabolic Research
Studying gene regulation and enzyme kinetics in Escherichia coli and related microbial hosts.
Principle of Action
IPTG functions as a non-hydrolyzable analog of allolactose. It binds to the lac repressor, inducing a conformational change that prevents the repressor from binding to the operator. This releases the transcriptional block, allowing RNA polymerase to transcribe downstream genes under the control of the lac promoter.
Chemical Identity
| Catalog Number | ASE-901 |
| CAS Number | 367-93-1 |
| Chemical Name | Isopropyl-β-D-thiogalactopyranoside |
| Molecular Formula | C₉H₁₈O₅S |
| Molecular Weight | 238.31 g/mol |
| Form | White crystalline powder |
Specification Highlights
| Purity (HPLC) | ≥99.5 % |
| Dioxane Content | None detected (Dioxane-Free) |
| Solubility | Soluble in water (5% w/v) |
| Quality Grade | Molecular Biology / Biotechnology Grade |
Storage & Handling
● Storage: Store at −20 °C in a cool, dry place.
● Protection: Keep container tightly closed and protected from light and moisture.
● Stability: Stable for a minimum of 2 years when stored under recommended conditions.
Ausamics Laboratory Division ensures rigorous quality control for every batch of ASE-901. Our "Dioxane-Free" guarantee eliminates hazardous solvent contamination for sensitive enzymatic assays.
TDS
Technical Data Sheet including preparation and stock concentration protocols.
SDS
Safety Data Sheet available for laboratory safety compliance.
COA
Batch-specific Certificate of Analysis verifying purity and solvent absence.
AuSaMicS Laboratory Division IPTG is intended for laboratory research and biotechnology development use only. Not for human or animal use.
Ausamics · Enzyme Substrates & Molecular Reagents · Cat No: ASE-901