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IPTG (Dioxane-Free) | ASE-901 | Lac Operon Inducer | AuSaMicS
$25.00 AUD
Documentation
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E. coli Cloning & Blue-White Screening Media Set
Get LB Agar, LB Broth, IPTG and X-Gal together, with configurable antibiotic selection and optional SOC recovery medium.
View E. coli Cloning Media Set →
IPTG (Isopropyl β-D-1-thiogalactopyranoside)
Non-Metabolisable Inducer for lac-Controlled Gene Expression & Blue-White Screening
Catalogue No: ASE-901-1
Non-Metabolisable Inducer for lac-Controlled Gene Expression & Blue-White Screening
Catalogue No: ASE-901-1
≥99.5% Purity (HPLC)
Dioxane-Free
Molecular Biology Grade
Australian Made
Batch QC Tested
Overview
IPTG is a synthetic, non-metabolisable molecular mimic of allolactose used as a potent inducer of the lac operon in bacterial gene expression systems. Because it cannot be hydrolysed by β-galactosidase, IPTG provides stable, sustained induction of lac-controlled genes throughout the growth cycle — unlike lactose itself, whose inducing effect declines as it is metabolised.
Applications
- Protein Expression: Induction of recombinant protein production in T7 and other lac-based bacterial expression systems
- Blue-White Screening: Used with X-Gal for visual identification of recombinant clones via lacZ α-complementation
- Metabolic & Regulatory Research: Studying gene regulation and enzyme kinetics in E. coli and related microbial hosts
Principle of Action
IPTG binds the lac repressor (LacI), inducing a conformational change that prevents the repressor from binding the operator. This releases the transcriptional block, allowing RNA polymerase to transcribe downstream genes under control of the lac promoter — independent of ongoing lactose metabolism.
Working Concentration & Stock Preparation
| Stock Solution | 100 mM – 1 M in water, filter-sterilised (0.22 µm), stored at -20°C |
| Working Concentration | 0.1–1.0 mM final (commonly ~0.5 mM; ~40 µL of 100 mM stock per standard agar plate) |
Add to culture media after autoclaving, once cooled to 45–50°C, to prevent thermal degradation.
Storage
| Powder | -20°C (long-term) or 2–8°C (working stock), desiccated, protect from moisture |
| Stock solution | -20°C, protected from light; stable for the shelf life stated on the Certificate of Analysis |
| Do not use if | Discolouration, clumping, or contamination is observed |
For laboratory research and biotechnology development use only. Not for human or animal use. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
Product Identification
| Product Name | IPTG |
| Catalogue Number | ASE-901-1 |
| CAS Number | 367-93-1 |
| Molecular Formula | C9H18O5S |
| Molecular Weight | 238.30 g/mol |
| Grade | Molecular Biology Grade |
| Manufacturer | AuSaMicS Pty Ltd, Melbourne, Australia |
Physical & Chemical Properties
| Appearance | White to off-white crystalline powder |
| Solubility | Freely soluble in water (≥100 mg/mL) |
| Purity | ≥99.5% (HPLC) |
| Dioxane Content | None detected (dioxane-free) |
| Storage | -20°C (long-term) or 2–8°C (working stock), desiccated, protect from moisture |
Mode of Action
Non-hydrolysable molecular mimic of allolactose; binds and inactivates the lac repressor (LacI), enabling constitutive expression from lac-controlled promoters/operators independent of ongoing lactose metabolism.
Quality Control — Performance Test
| Test | Organism / System | Expected Result | Acceptance Criterion |
|---|---|---|---|
| Functional induction | E. coli DH5α (lacZΔM15, pUC19), 37°C 16-18h on LB+Amp+IPTG+X-Gal | White colonies (recombinant) vs blue (control) | Clear functional induction / expected blue-white differentiation in a compatible lacZ screening system |
Literature & References
- Sambrook, J. & Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual, 3rd edn. CSHL Press.
- Vieira, J. & Messing, J. (1982). The pUC plasmids. Gene. 19(3):259-268.
- Ullmann, A., Jacob, F. & Monod, J. (1967). J Mol Biol. 24(2):339-343.
For laboratory and research use only. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
GHS Classification: Warning (irritant) | Signal Word: Warning | Pictogram: GHS07
Section 1 — Identification
| Product Name | IPTG |
| Catalogue Number | ASE-901-1 |
| CAS Number | 367-93-1 |
| Intended Use | Laboratory reagent — molecular biology research use only. Not for human or animal use. |
| Supplier | AuSaMicS Pty Ltd | 36/25 Trafalgar Road, Epping VIC 3076 |
| ABN | 56 676 640 467 |
| Emergency Phone | +61 412 520 598 | Poisons Information Centre: 13 11 26 (Australia, 24 hr) |
| support@ausamics.com |
Section 2 — Hazard Identification
Hazard Statements: H315 – Causes skin irritation. H319 – Causes serious eye irritation. H335 – May cause respiratory irritation.
Precautionary Statements: P261 Avoid breathing dust. P264 Wash hands thoroughly after handling. P271 Use only outdoors or in a well-ventilated area. P280 Wear protective gloves/eye protection. P304+P340 IF INHALED: remove to fresh air and keep at rest. P305+P351+P338 IF IN EYES: rinse cautiously with water for several minutes; remove contact lenses if present. P362+P364 Take off contaminated clothing and wash before reuse.
Section 3 — Composition
| Component | CAS No. | Concentration |
|---|---|---|
| IPTG | 367-93-1 | Single-constituent substance (see COA for lot-specific assay) |
Section 4 — First Aid
| Inhalation | Remove to fresh air. Seek medical attention if breathing is difficult. |
| Skin Contact | Wash with plenty of soap and water. Seek medical advice if irritation persists. |
| Eye Contact | Rinse cautiously with water for several minutes. Remove contact lenses if present. |
| Ingestion | Rinse mouth. Do not induce vomiting. Seek medical advice if unwell. |
Section 7 — Handling & Storage
| Storage | -20°C (long-term) or 2–8°C (working stock), desiccated, protect from moisture |
| Handling | Handle in a well-ventilated area. Avoid generating or inhaling dust. Wash hands after handling. |
Section 8 — Personal Protection
| Respiratory | Dust mask (P1/P2) if handling in poorly ventilated area |
| Eye protection | Safety glasses required |
| Gloves | Nitrile laboratory gloves |
Section 13 — Disposal
Dispose of as laboratory chemical waste per local EPA regulations. Do not discharge to sewer in bulk quantity.
Section 14 — Transport
Not classified as Dangerous Goods for transport (ADG/IATA/IMDG) in the quantities and packaging typically supplied.
⬇ Download Full SDS — 16 Sections (PDF)Prepared in accordance with Australian WHS Regulations and GHS 7th Edition. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
Certificates of Analysis are batch-specific
Request / Find COA by Lot →
This tab does not show a generic or "typical" result set, because a COA belongs to one manufactured lot — not to the product in general. To obtain the actual Certificate of Analysis for the container you have (or are about to order), contact us with the product code and lot number printed on the label.
| Product Code | ASE-901-1 |
| Lot Number | Printed on your container label |
What's tested on every lot
| Test Parameter | Method |
|---|---|
| Appearance | Visual |
| Identity | TLC / HPLC |
| Purity | HPLC (specification: ≥99.5%) |
| Moisture | Karl Fischer / loss on drying |
| Dioxane content | GC (specification: not detected) |
| Functional performance | Blue-white screening test, E. coli DH5α + pUC19 |
This table lists what each lot is tested against — it is not a result. Your lot's actual measured values are stated on its own Certificate of Analysis.
Authorisation
| Released by | Hassan Salimi |
| Title | Founder and Technical Director |
| Organisation | AuSaMicS Pty Ltd |
Need a batch-specific COA?
Contact us with your order number, product code and lot number and we will issue the corresponding COA within 1 business day.
support@ausamics.com | +61 412 520 598
Contact us with your order number, product code and lot number and we will issue the corresponding COA within 1 business day.
support@ausamics.com | +61 412 520 598
Certificates of Analysis are issued per manufactured lot by AuSaMicS Pty Ltd and are valid only for the batch identified on them. Results are not transferable between lots. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au