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Irgasan (Triclosan) Powder | ASA-2013 | Selective Media Supplement | AuSaMicS
$75.00 AUD
Irgasan (Triclosan)
High-Purity Selective Antimicrobial Supplement for CFC Agar (Pseudomonas) and CIN Agar (Yersinia) | Cat. No. ASA-2013
Cat. No. ASA-2013 CAS 3380-34-5 C₁₂H₇Cl₃O₂ MW 289.54 g/mol ≥98.0% Purity (HPLC) ✓ ISO 13720:2010 | ISO 10273:2017 🇦🇺 Made in Melbourne ⚡ Same-Week Dispatch
Cat. No.
ASA-2013
CAS
3380-34-5
MW
289.54 g/mol
Purity
≥98.0% HPLC
Storage
2–8°C, dark, dry
📄 Full DocumentationCOA, TDS & SDS every batch
🇦🇺 Australian StockNo import delays
⚡ Same-Week DispatchMelbourne warehouse
🔬 Technical SupportDirect from our team
What Is Irgasan (Triclosan) & Why Is It Used in Selective Media?
Irgasan (trade name for triclosan, CAS 3380-34-5) is a chlorinated bisphenol ether with potent broad-spectrum antibacterial activity. In laboratory microbiology, it is used at very low concentrations (1–4 mg/L) as a selective supplement to suppress unwanted background flora while allowing target organisms to recover.
Irgasan is a key selective agent in two important standard media: CFC Agar for Pseudomonas spp. isolation (ISO 13720:2010) and CIN Agar for Yersinia enterocolitica isolation (ISO 10273:2017).
Mode of Action — FabI Inhibition
FabI Inhibition → Fatty Acid Synthesis Arrest → Bacteriostatic Effect
Triclosan specifically inhibits enoyl-acyl carrier protein reductase (FabI) — a key enzyme in the Type II bacterial fatty acid synthesis (FAS-II) pathway. By binding to the FabI-NAD⁺ complex, it blocks the fatty acid elongation cycle, preventing synthesis of the long-chain fatty acids essential for bacterial membrane integrity.
At sub-MIC concentrations used in selective agar, this causes bacteriostatic inhibition of susceptible background organisms (most Gram-positives and many Gram-negatives), while target organisms with reduced FabI sensitivity (Pseudomonas spp., Yersinia enterocolitica) continue to grow.
Triclosan specifically inhibits enoyl-acyl carrier protein reductase (FabI) — a key enzyme in the Type II bacterial fatty acid synthesis (FAS-II) pathway. By binding to the FabI-NAD⁺ complex, it blocks the fatty acid elongation cycle, preventing synthesis of the long-chain fatty acids essential for bacterial membrane integrity.
At sub-MIC concentrations used in selective agar, this causes bacteriostatic inhibition of susceptible background organisms (most Gram-positives and many Gram-negatives), while target organisms with reduced FabI sensitivity (Pseudomonas spp., Yersinia enterocolitica) continue to grow.
Primary Applications
🧪
CFC Agar
Pseudomonas spp. isolation — ISO 13720
🧬
CIN Agar
Yersinia enterocolitica — ISO 10273
🍕
Food Safety
Chilled food, poultry, meat, water
🌿
Environmental
Water & environmental monitoring
📊
Research
FabI inhibition & selective media studies
🏭
Industrial QC
Food processing plant monitoring
Key Selective Media Using Irgasan / Triclosan
| Medium | Irgasan Conc. | Target Organism | Other Selective Agents | Standard |
|---|---|---|---|---|
|
CFC Agar (Cephalothin-Fucidin-Cetrimide) |
1–4 mg/L | Pseudomonas spp. | Cephalothin 50 mg/L, Sodium Fusidate 10 mg/L, Cetrimide 10 mg/L | ISO 13720:2010 |
|
CIN Agar (Cefsulodin-Irgasan-Novobiocin) |
4 mg/L | Yersinia enterocolitica | Cefsulodin 15 mg/L, Novobiocin 2.5 mg/L | ISO 10273:2017 |
ⓘ Important distinction: CFC Agar (Cephalothin-Fucidin-Cetrimide) is for Pseudomonas spp. isolation. CIN Agar (Cefsulodin-Irgasan-Novobiocin) is for Yersinia enterocolitica. Both use Irgasan but at different concentrations and with different co-selective agents.
Stock Solution Preparation
1
Prepare stock solution: Dissolve 0.1 g Irgasan (ASA-2013) in 10 mL of 95% ethanol = 10 mg/mL stock. Irgasan is practically insoluble in water (~10 mg/L) — ethanol or DMSO must be used.
2
Filter-sterilise: Pass through a 0.22 µm PTFE or nylon filter. Do NOT autoclave the stock — heat degrades Irgasan.
3
Add to cooled agar: Cool autoclaved agar base to ≤50°C. Add stock aseptically:
• CFC Agar at 4 mg/L → add 0.4 mL stock per litre
• CIN Agar at 4 mg/L → add 0.4 mL stock per litre
• CFC Agar at 4 mg/L → add 0.4 mL stock per litre
• CIN Agar at 4 mg/L → add 0.4 mL stock per litre
4
Pour immediately. Stock solution: stable 1 month at 2–8°C in dark sealed container.
⚠️ Never add undissolved Irgasan powder directly to aqueous media. Insolubility in water means homogeneous distribution cannot be achieved without prior dissolution in ethanol or DMSO.
Frequently Asked Questions
❓ Is ASA-2013 the same compound as ASA-2058?
Yes — both ASA-2013 and ASA-2058 are Irgasan / Triclosan (CAS 3380-34-5, ≥98.0% HPLC). They are the same compound supplied under different catalogue numbers for different pack sizes or customer requirements. The chemistry, purity, and applications are identical.
❓ Is CFC Agar for Pseudomonas or Campylobacter?
CFC Agar (Cephalothin-Fucidin-Cetrimide) is for Pseudomonas spp. isolation per ISO 13720:2010 — developed by Mead and Adams for chilled food and poultry. It is not for Campylobacter. Campylobacter selective media use different formulations (mCCDA, Preston Agar, Campy-BAP).
❓ Can I use DMSO instead of ethanol?
Yes. DMSO is suitable for Irgasan stock preparation. Use the same approach: dissolve, filter-sterilise (0.22 µm), add to cooled agar. Verify DMSO at the working concentration does not affect your specific method.
❓ Is ASA-2013 equivalent to Sigma-Aldrich 72779?
Yes — same compound (CAS 3380-34-5), same purity grade (≥98.0% HPLC). Full COA supplied with every batch for side-by-side verification.
❓ Why does Irgasan have a low melting point (56–60°C)?
This is a key handling consideration. Irgasan's melting point is 56–60°C, which means it must be stored below 30°C to remain as a powder. Never store near heat sources. Ensure agar is cooled to ≤50°C before adding the stock solution to avoid degradation.
Cross-Reference / Equivalents
| Manufacturer | Product Name | Cat. No. | Notes |
|---|---|---|---|
| Sigma-Aldrich (Merck) | Irgasan | 72779 | Equivalent grade; CAS 3380-34-5 |
| Merck Millipore | Triclosan | 1.01776 | Equivalent grade |
| TCI Chemicals | Triclosan | T1421 | Equivalent grade |
| Cayman Chemical | Triclosan | 10008361 | Equivalent research grade |
AuSaMicS ASA-2013 — manufactured in Melbourne, same-week dispatch, no import delays, COA included.
Related Products
Chemical & Physical Properties
| Product Name | Irgasan (Triclosan) |
| Catalogue Number | ASA-2013 |
| IUPAC Name | 5-Chloro-2-(2,4-dichlorophenoxy)phenol |
| Synonyms | Triclosan; Irgasan DP 300; 2,4,4'-Trichloro-2'-hydroxydiphenyl ether |
| CAS Number | 3380-34-5 |
| Molecular Formula | C₁₂H₇Cl₃O₂ |
| Molecular Weight | 289.54 g/mol |
| Grade | Laboratory Grade |
| Purity | ≥98.0% (HPLC) |
| Appearance | White to off-white crystalline powder |
| Melting Point | 56–60°C ⚠ Store below 30°C |
| Solubility (water) | ~10 mg/L at 20°C (practically insoluble) |
| Solubility (ethanol) | Freely soluble (>10 g/100 mL) |
| Solubility (DMSO) | Freely soluble (>50 mg/mL) |
| log P (octanol/water) | 4.76 (highly lipophilic) |
| pKa | 7.9 (phenol group) |
Validated Use Concentrations
| Medium | Target | Final Conc. | Stock Addition (10 mg/mL) | Standard |
|---|---|---|---|---|
| CFC Agar (Pseudomonas) | Pseudomonas spp. | 1–4 mg/L | 0.1–0.4 mL / L | ISO 13720:2010 |
| CIN Agar (Yersinia) | Yersinia enterocolitica | 4 mg/L | 0.4 mL / L | ISO 10273:2017; Schiemann 1979 |
Storage & Stability
| Storage Temperature | 2–8°C; protect from heat and light |
| Container | Tightly sealed, light-resistant container |
| Critical Note | Melting point 56–60°C — store well below 30°C; never near heat sources |
| Shelf Life (powder) | ≥3 years from manufacture date under correct storage |
| Stock Solution Stability | 1 month at 2–8°C in dark sealed container (ethanol or DMSO) |
Literature & References
| # | Reference |
|---|---|
| 1 | Mead GC, Adams BW. A selective medium for the rapid isolation of pseudomonads associated with poultry meat spoilage. Br Poult Sci. 1977;18(6):661–670. [Original CFC Agar development for Pseudomonas] |
| 2 | ISO 13720:2010. Meat and meat products — Enumeration of presumptive Pseudomonas spp. Geneva: ISO; 2010. [CFC Agar standard for Pseudomonas] |
| 3 | Schiemann DA. Synthesis of a selective agar medium for Yersinia enterocolitica. Can J Microbiol. 1979;25(11):1298–1304. [Original CIN Agar formulation with Irgasan] |
| 4 | ISO 10273:2017. Food microbiology — Horizontal method for detection of pathogenic Yersinia enterocolitica. Geneva: ISO; 2017. |
| 5 | McMurry LM, Oethinger M, Levy SB. Triclosan targets lipid synthesis. Nature. 1998;394(6693):531–532. [FabI as triclosan's primary target] |
| 6 | Heath RJ, et al. Mechanism of triclosan inhibition of bacterial fatty acid synthesis. J Biol Chem. 1999;274(16):11110–11114. [FabI-NAD⁺ binding mechanism] |
📄 Full 16-section GHS SDS available (Australian WHS Regulations 2023 / GHS 7th Edition) — support@ausamics.com.au
Section 1 — Identification
| Product Name | Irgasan (Triclosan) |
| Catalogue No. | ASA-2013 |
| Supplier | AuSaMicS Pty Ltd | ABN 56 676 640 467 |
| Address | 31 Longview CT, Thomastown VIC 3074, Australia |
| Emergency | Poisons Information Centre: 13 11 26 (24 hr) |
| Phone | +61 412 520 598 |
| support@ausamics.com.au |
Section 2 — Hazard Identification (GHS 7th Ed / WHS 2023)
| GHS Classification | Acute Tox. 4 (oral) — H302: Harmful if swallowed Skin Irrit. 2 — H315: Causes skin irritation Eye Irrit. 2 — H319: Causes serious eye irritation Skin Sens. 1 — H317: May cause an allergic skin reaction Aquatic Acute 1 — H400: Very toxic to aquatic life Aquatic Chronic 1 — H410: Very toxic to aquatic life with long-lasting effects |
| Signal Word | WARNING |
| GHS Pictograms | ⚠️ Exclamation mark | 🌿 Environmental hazard (significant) |
⚠️ Aquatic Hazard (H400/H410): Irgasan is very toxic to aquatic organisms and is persistent in the environment. It can form chlorophenols and dioxins under UV/chlorination. Do NOT dispose of solutions down the drain. All waste containing Irgasan must go to a licensed chemical waste contractor.
Sections 4–8 — First Aid, Fire & PPE
| Inhalation | Remove to fresh air. Seek medical attention if symptoms persist. |
| Skin Contact | Remove contaminated clothing. Wash with soap and water ≥15 min. Seek advice if rash develops (sensitiser — H317). |
| Eye Contact | Irrigate with water ≥15 min, hold eyelids apart. Seek immediate medical attention. |
| Ingestion | Do NOT induce vomiting. Rinse mouth. Call Poisons Centre 13 11 26. Seek medical attention. |
| Extinguishing Media | Water spray, foam, dry powder, CO₂ |
| Respiratory PPE | P2 particulate filter when weighing or handling bulk powder |
| Eye Protection | Safety glasses or chemical splash goggles |
| Skin Protection | Nitrile gloves (≥0.11 mm); lab coat; closed footwear. Double-glove if frequent handling (sensitiser) |
Sections 12–15 — Ecology, Disposal & Regulatory
| Aquatic Toxicity | Very toxic to aquatic life (H400/H410). Do not discharge to drains, sewers, or waterways. |
| Persistence | Biodegrades slowly under aerobic conditions. Can form chlorophenols and dioxins under UV/chlorination. |
| Disposal | Licensed chemical waste contractor required. Do not drain-dispose under any circumstances. |
| Transport | Not classified as dangerous goods at laboratory pack sizes — ADG, IMDG, IATA |
| AICIS Status | Listed on Australian Inventory of Industrial Chemicals (AIIC) |
| EU Status | Restricted in cosmetics (EU Regulation 1223/2009). This product is laboratory reagent grade ONLY. |
| Regulatory Framework | Australian WHS Regulations 2023 | GHS 7th Edition | Safe Work Australia COP 2021 |
Quality Specifications
| Parameter | Specification | Method |
|---|---|---|
| Appearance | White to off-white crystalline powder; free-flowing | Visual |
| Purity | ≥98.0% | HPLC |
| Melting Point | 56–60°C | DSC / capillary method |
| Loss on Drying | ≤1.0% | Loss on drying (60°C / 2h) |
| Identity | Consistent with reference IR spectrum (CAS 3380-34-5) | IR spectroscopy |
| Heavy Metals | ≤20 ppm | ICP-OES |
| Selective Performance (CFC Agar) | Inhibits background flora; permits Pseudomonas aeruginosa ATCC 27853 growth at 4 mg/L | Plate performance test |
| Selective Performance (CIN Agar) | Inhibits background flora; permits Y. enterocolitica growth at 4 mg/L | Plate performance test |
| Batch COA | Available for every production lot | Included with order |
Manufacturing & Documentation
Supplied by AuSaMicS Pty Ltd
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ HPLC purity verification per batch
✓ COA, TDS, SDS included with every order
✓ Australian stock — no import delays
✓ Technical support for CFC / CIN Agar media preparation
31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467
✓ HPLC purity verification per batch
✓ COA, TDS, SDS included with every order
✓ Australian stock — no import delays
✓ Technical support for CFC / CIN Agar media preparation
⚠️ Intended Use: For laboratory and industrial QC use only. Not for cosmetic, therapeutic, food, or consumer applications. Very toxic to aquatic life — all waste must be disposed via licensed chemical waste contractor only.