ITC Broth Base (AS-1255) | Yersinia Enrichment Broth
$95.00 AUD
AuSaMicS Life Science • Pathogen Specialized
ITC Broth Base (Irgasan Ticarcillin Chlorate)
Selective Enrichment for Yersinia enterocolitica
A highly selective enrichment broth optimized for the recovery of Yersinia enterocolitica from food and clinical samples. Developed to suppress competing Gram-positive and Gram-negative microflora through a synergistic antibiotic matrix.
AS-1255
🧪 ISO 10273 Compliant
✓ Synergistic Selectivity
🔬 Food & Clinical Use
Selectivity: High (Multi-Antibiotic) Incubation: 25°C / 48h Prep: 26.3g/L Yield
Yersinia Specialist
Potent Inhibition - Irgasan & Ticarcillin fortified
ISO Compliant - Validated for ISO 10273
Optimized Growth - Yeast extract enriched
Specific Recovery - From complex matrices
Wauters Formulation
Diagnostic Precision Standard
🧪 Formulation & Selective Profile
📋 Formulation (per Liter)
| Component | Concentration | Role |
|---|---|---|
| Tryptone / Peptone | 10.0 g | Amino acid source |
| Yeast Extract | 1.0 g | B-vitamin source |
| Magnesium Chloride | 10.0 g | Ionic balance |
| Potassium Chlorate | 1.0 g | Selective agent |
| Malachite Green | 0.01 g | Inhibits Gram-positives |
*Note: Ticarcillin and Irgasan supplements required post-sterilization for full ITC performance.
Diagnostic Application
Target Pathogen
Primarily used for Y. enterocolitica (Serotypes O:3 and O:9) in pork products and environmental samples.
Selective Synergy
The combination of Chlorate and Malachite Green with specific antibiotics ensures minimal background growth.
📋 Technical Profile
| Catalogue No. | AS-1255 |
| Final pH | 6.9 ± 0.2 |
| Preparation | 26.3g per Liter |
| Compliance | ISO 10273 |
⚗️ Laboratory Prep
- 1 Suspend 26.3g in 1L deionized water.
- 2 Autoclave at 121°C for 15 min.
- 3 Cool and aseptically add Irgasan & Ticarcillin supplements.