Jenner’s Stain (Eosin–Methylene Blue Stain)
Jenner’s Stain (Eosin–Methylene Blue Stain)
Catalog Number: ASC-1033
CAS Number: 62851-42-7
Molecular Weight: —
Molecular Formula: —
Overview
Jenner’s Stain is a classical Romanowsky-type biological stain composed of eosin and methylene blue, used for the differential staining of blood smears and cellular specimens. It provides clear visual contrast between nuclei, cytoplasm, erythrocytes, and leukocyte granules, making it suitable for routine microscopic examination.
This stain is widely used in hematology, cytology, microbiology teaching, and research laboratories for general morphology studies. Jenner’s Stain offers rapid staining and straightforward preparation, making it a foundational stain for routine laboratory workflows.
Principle of Staining
-
Methylene blue (basic dye): stains nuclei and nucleic acids blue to purple
-
Eosin (acidic dye): stains cytoplasm, hemoglobin, and eosinophilic structures pink to red
The combined action of these dyes produces balanced contrast for effective cellular differentiation.
Typical Applications
-
Peripheral blood smear staining
-
Visualization of erythrocytes, leukocytes, and platelets
-
Cytological examination of body fluids
-
General cellular morphology studies
-
Teaching and training in microscopy and hematology
Key Features
-
Romanowsky-type eosin–methylene blue formulation
-
Rapid and consistent staining performance
-
Clear nuclear and cytoplasmic differentiation
-
Suitable for routine laboratory and educational use
Storage & Handling
-
Store tightly sealed at 15–30 °C
-
Protect from moisture and direct light
-
Prepare staining solutions according to laboratory protocols
Intended Use
For laboratory and research use only.
Not intended for diagnostic, clinical, therapeutic, human, or veterinary use.
Quality & Compliance
-
Manufactured under controlled conditions
-
Suitable for routine laboratory applications
-
COA and SDS available upon request