King Agar A (Pseudomonas Agar P) | Pyocyanin Production – Ausamics

King Agar A - Pseudomonas Agar P - Tech Agar - Pseudomonas Agar for Pyocyanin

$39.00 AUD

AuSaMicS Life Science • Pseudomonas Identification Media

King Agar A

Pyocyanin Production Medium for Pseudomonas aeruginosa

Selective and differential solid medium formulated to enhance and demonstrate pyocyanin production by Pseudomonas aeruginosa. King Agar A remains the classical reference medium for blue-green pigment detection in clinical, environmental, water, and food microbiology workflows.

AS-1259 🧪 Differential Agar ✓ Pyocyanin Detection 🔬 Pseudomonas ID

Target: P. aeruginosa Readout: Blue-green diffusible pigment Use: Clinical, environmental, food, and water microbiology

Pseudomonas Media Specialist

Pyocyanin Focus - Designed to stimulate blue-green pigment

Classical Reference Medium - King, Ward & Raney formulation

Clear Visual Readout - No UV required

Differentiation Utility - Distinguishes pyocyanin producers from other pseudomonads

Routine Laboratory Use - Widely used in confirmation workflows

Gold-Standard Pyocyanin Medium

For visible pigment-based confirmation

🧪 Complete Formulation & Pigment Principle

📋 Composition (per Liter)

Ingredient	Concentration	Primary Function	Contribution
Peptone / Gelatin Peptone	20.0 g	Nutrient source	Supports robust growth of pseudomonads
Potassium Sulfate (K₂SO₄)	10.0 g	Pigment stimulant	Enhances pyocyanin biosynthesis
Magnesium Sulfate	0.7 g anhydrous or 1.4 g heptahydrate	Pigment stimulant	Promotes blue-green pyocyanin production
Glycerol	10.0 mL	Carbon source	Supports enhanced pigment expression
Agar	15.0 g	Solidifying agent	Plate-based colony observation and pigment diffusion

Final pH: 7.2 ± 0.2 at 25°C

⚗️ Principle of the Medium

Pyocyanin Stimulation
Magnesium and potassium salts strongly stimulate pyocyanin biosynthesis

Glycerol-Based Expression
Glycerol supports characteristic pigment development

Visible Pigment Differentiation
Pyocyanin is visible in daylight without UV illumination

Why King Agar A? It is the classic choice when reliable demonstration of pyocyanin is needed for presumptive or confirmatory identification of Pseudomonas aeruginosa.

Complete Pseudomonas Detection Media Portfolio

Medium	Type	Main Readout	Best Application	Target Use	Key Advantage
King Agar A	Differential pigment medium	Pyocyanin blue-green pigment	Confirmation of P. aeruginosa	Clinical, food, water, environmental samples	Gold-standard pyocyanin demonstration
King Agar B	Fluorescent pigment medium	Fluorescein / pyoverdine	Differentiation of fluorescent pseudomonads	Pseudomonas screening	Highlights fluorescence rather than pyocyanin
Cetrimide Agar	Selective isolation agar	Selective growth plus pigment support	Primary selective isolation of P. aeruginosa	Clinical and water microbiology	Higher selectivity than King media
Pseudomonas Selective Agar	Selective medium	Selective recovery of pseudomonads	Routine isolation workflows	Food and environmental samples	Improved suppression of competitors
General-Purpose Agar	Non-selective medium	Growth only	Routine subculture	Broad bacterial cultivation	Useful when pigment stimulation is not required

Strategic Medium Selection for Pseudomonas aeruginosa Confirmation

✅ King Agar A Advantages

✓ Classic Reference Medium: Long-established standard for pyocyanin detection
✓ Highly Useful for Confirmation: Strong blue-green pigment supports presumptive ID
✓ No UV Needed: Pigment visible to the naked eye
✓ Differentiates from Fluorescent Pseudomonads: Unlike King B, focuses on pyocyanin rather than fluorescein
✓ Useful Across Sectors: Clinical, environmental, water, and food microbiology

⚠️ Method Considerations

▲ Not Strongly Selective: May need selective primary isolation before confirmation
▲ Pigment May Intensify Over Time: Reading at multiple time points can improve interpretation
▲ Not All Pseudomonads Produce Pyocyanin: Best used specifically for P. aeruginosa workflows
▲ Should Be Interpreted with Other Tests: Confirmation may still require complementary methods

🎯 Best-Fit Applications

◆ Clinical Microbiology: Wounds, burns, respiratory isolates
◆ Water Testing: Confirmation of pyocyanin-producing isolates
◆ Food & Beverage QC: Detection of P. aeruginosa where relevant
◆ Environmental Microbiology: Surface and contamination tracing workflows

📊 Typical Colony Appearance

Organism / Group	Typical Colony Appearance	Pigment Reaction	Notes
Pseudomonas aeruginosa	2–4 mm, flat, smooth, blue-green to dark blue, metallic sheen	Strong diffusible blue-green pigment	Typical pyocyanin-positive reaction
Other Pseudomonas spp.	Cream, pale yellow, or non-pigmented colonies	Usually no pyocyanin	May fluoresce on King B instead
Enterobacteriaceae	Usually inhibited or small pale colonies	No characteristic pigment	Atypical growth should be interpreted cautiously

🔬 Quality Control & Performance Guidance

Typical Target:
Pseudomonas aeruginosa
pyocyanin-producing isolates

Incubation:
35–37°C
18–72 h, with pigment intensification over time

Observation:
Daylight reading is sufficient
UV illumination not required

Storage:
Prepared plates at 2–8°C
Protect from light, use within 4 weeks

Positioning: A classic differential agar for confirmation of P. aeruginosa by pyocyanin production in clinical, environmental, food, and water microbiology workflows.

📋 Technical Specifications

Catalogue Number	AS-1259
Medium Type	Differential pigment-production agar
Primary Target	Pseudomonas aeruginosa
Key Readout	Diffusible blue-green pyocyanin pigment
Final pH	7.2 ± 0.2 (at 25°C)
Dehydrated Medium Weight	Approx. 41.4–42.1 g/L plus glycerol
Glycerol Addition	10 mL/L
Sterilization	121°C for 15 minutes
Incubation	35–37°C for 18–72 h
Prepared Plate Storage	2–8°C, protected from light, up to 4 weeks

🧫 Primary Applications

✓ Confirmation of Pseudomonas aeruginosa by pyocyanin production
✓ Differentiation from fluorescent but non-pyocyanin-producing pseudomonads
✓ Clinical microbiology specimens such as wounds, burns, and respiratory samples
✓ Water, food, and environmental confirmation workflows

⚗️ Preparation Protocol

1. Suspend 41.4–42.1 g dehydrated medium in 990 mL purified water.
2. Add 10 mL pure glycerol.
3. Heat with frequent agitation and boil for 1 minute.
4. Autoclave at 121°C for 15 minutes.
5. Cool to 45–50°C, mix well, and pour plates.

📦 Storage & Intended Use

Dehydrated medium: Store in a cool, dry place below 30°C.

Prepared plates: Store at 2–8°C, protected from light.

Use period: Use within 4 weeks for optimal pigment performance.

Notice: For clinical, environmental, and food microbiology use only.

AuSaMicS Life Science: Professional microbiology media for clinical microbiology, environmental monitoring, food safety, research, and specialized organism identification workflows. Product information is provided for laboratory and technical evaluation purposes only.