King’s B Medium Base Powder | Pseudomonas Fluorescence Medium

King’s B Medium Base (AS-1260) | Pseudomonas Agar F for Fluorescein

$39.00 AUD

AuSaMicS Life Science • Diagnostic Pigmentation Media

King’s B Medium Base

Pseudomonas Agar F / Flo Agar

A specialized differential medium designed to enhance the production of fluorescein (pyoverdin). A critical tool for the definitive identification of Pseudomonas species in clinical and environmental samples.

AS-1260 ✨ UV Fluorescent ✓ USP/EP Compliant 🧬 Pigment Enhancement

Target: Pyoverdin Production Incubation: 25-30°C pH: 7.2 ± 0.2

Diagnostic Performance

Fluorescein (+)
Yellow-Green Pigment

UV Visualization
Bright Fluorescence at 366nm

Optimized Peptones
Rich in Phosphorus for Pigment

🔬 Key Applications

Clinical Diagnostics

Differentiation of Pseudomonas aeruginosa from other non-fermenting Gram-negative bacilli.

Pharma Quality Control

Validated for use in USP/EP microbial limit tests for the detection of P. aeruginosa.

🧪 Nutrient Formulation (per Liter)

Ingredient	Concentration	Function
Peptone Mixture	20.0 g	Amino Acids (High Phosphorous)
Dipotassium Phosphate	1.5 g	Phosphorous Source for Pigment
Magnesium Sulfate	1.5 g	Cation Cofactor for Fluorescein
Bacteriological Agar	15.0 g	Solidifying Agent

*Note: Requires addition of 10-15 mL/L of Glycerol (not included in base powder).

📋 Technical Specs

Catalog No.	AS-1260
Final pH	7.2 ± 0.2
Yield	38.0 g / L
Appearance	Light Amber (Clear)

⚗️ Laboratory Prep

1 Suspend 38.0g in 1L purified water. Add 10-15mL Glycerol.
2 Heat to boiling to dissolve agar completely.
3 Autoclave at 121°C for 15 minutes.
4 Cool to 45°C and pour into sterile plates. Store at 2-8°C.