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Kligler Iron Agar for Enterobacteriaceae Differentiation | AS-1261
$45.00 AUD
Kligler Iron Agar (KIA)
Selective differential medium for Enterobacteriaceae identification based on carbohydrate fermentation and hydrogen sulfide production.
| Catalogue Number | AS-1261 |
| Manufacturer | AuSaMicS Pty Ltd, Melbourne, Australia |
| Medium Type | Differential solid medium (slant) |
| Final pH | 7.4 ± 0.2 at 25°C |
| HS Code | 3821.00.00 |
Intended Use
Kligler Iron Agar (KIA) is used for the differentiation of Gram-negative enteric bacteria based on glucose and lactose fermentation, gas production, and hydrogen sulfide (H₂S) formation. It is widely applied in clinical microbiology, food safety testing, and water analysis workflows.
Principle of the Medium
- Glucose (0.1%): Limited concentration enables detection of glucose fermentation via initial acid production.
- Lactose (1.0%): Sustained acid production differentiates lactose fermenters.
- Phenol Red: pH indicator — yellow under acidic conditions, red under alkaline.
- Sodium Thiosulfate + Ferric Ammonium Citrate: Detect hydrogen sulfide production as black precipitate.
- Peptones: Provide nitrogen and support growth of non-fermenters.
Interpretation of Results
| Reaction | Slant | Butt | Interpretation |
|---|---|---|---|
| Yellow/Yellow | Acid | Acid | Glucose + Lactose fermentation |
| Red/Yellow | Alkaline | Acid | Glucose fermentation only |
| Red/Red | Alkaline | Alkaline | No fermentation |
| Black precipitate | — | — | H₂S production |
| Gas formation | — | Cracks/lifting | Gas production |
Composition (per litre)
| Component | CAS | Amount |
|---|---|---|
| Peptone | 73049-73-7 | 20.0 g |
| Lactose | 63-42-3 | 10.0 g |
| Glucose | 50-99-7 | 1.0 g |
| Sodium Chloride | 7647-14-5 | 5.0 g |
| Sodium Thiosulfate | 7772-98-7 | 0.3 g |
| Ferric Ammonium Citrate | 1185-57-5 | 0.5 g |
| Phenol Red | 143-74-8 | 0.025 g |
| Agar | 9002-18-0 | 12.0 g |
Preparation
- Dissolve 48.8 g in 1 L purified water.
- Heat with agitation until fully dissolved.
- Sterilise at 121°C for 15 minutes.
- Cool and dispense into tubes to form slants.
Storage
Store dehydrated medium at 15–30°C, protected from moisture and light. Prepared medium should be stored at 2–8°C and used within recommended shelf life.
Applications
- Clinical microbiology — Enterobacteriaceae differentiation
- Food microbiology — pathogen screening
- Water testing — coliform differentiation workflows
Equivalent Products
- Oxoid CM0033
- BD Difco 213730
- Merck 1.03981
For laboratory use only. Not for food, pharmaceutical, or therapeutic use.