LB Agar (Miller) (AS-1267) – High-Salt Medium for Bacterial Culture

LB Agar (Miller) is the most widely used general-purpose solid culture medium for the routine cultivation, cloning, and maintenance of recombinant Escherichia coli strains in molecular biology laboratories. The Miller formulation (1972) contains 10.0 g/L sodium chloride — the global standard for routine E. coli work.

Fully compatible with common antibiotics, IPTG/X-gal reporter systems, and all standard laboratory E. coli strains. Supports rapid bacterial growth, high cell density, and reliable plasmid maintenance.

• Routine Plating: DH5α, BL21, TOP10, JM109, XL1-Blue and other standard E. coli strains
• Antibiotic Selection: Ampicillin, kanamycin, chloramphenicol, tetracycline, gentamicin selection plates
• Blue–White Screening: X-gal (40 µg/mL) + IPTG (0.1 mM) for recombinant colony identification
• Plasmid Propagation: Colony isolation, transformation recovery plating, and overnight culture preparation
• Phage Work: Bacteriophage propagation, plaque assay overlay plates
• Teaching: Standard molecular biology instruction and microbiology cultivation

1. Suspend 40.0 g in 1 litre of demineralised or purified water.
2. Heat with agitation until fully dissolved.
3. Adjust pH to 7.0 ± 0.2 if required (1 M NaOH or 1 M HCl).
4. Sterilise by autoclaving at 121 °C for 15 minutes.
5. Cool to 45–50 °C.
6. Add filter-sterilised antibiotics or supplements aseptically.
7. Dispense 20–25 mL per 90 mm sterile Petri dish.
8. Allow to solidify on a level surface before use.

Total: 40.0 g/L  |  pH: 7.0 ± 0.2 at 25 °C

Not classified as hazardous according to GHS criteria (AU WHS Regulations 2011). No hazard pictograms, signal word, or hazard statements required. Standard laboratory precautions apply.

Autoclave inoculated plates (121 °C, 30 min) before disposal. Dispose of unused powder as general laboratory waste in accordance with local regulations.

Not classified as dangerous goods under ADG, IATA, or IMDG. No special transport requirements.