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AS-1269
LB Broth (Lennox) (AS-1269) – Low Salt Medium for Molecular Biology
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LB Broth (Lennox)
Reduced-Salt Liquid Culture Medium for Molecular Biology Applications
Catalogue No: AS-1269
Reduced-Salt Liquid Culture Medium for Molecular Biology Applications
Catalogue No: AS-1269
E. coli & Molecular Biology
Cloning & Transformation
Reduced NaCl (5 g/L)
Australian Made
Batch QC Tested
Overview
LB Broth (Lennox) is a nutritionally rich, non-selective liquid culture medium developed by Lennox in 1955 for the growth and maintenance of Escherichia coli in molecular biology laboratories. It is particularly suitable for recombinant E. coli K-12 derivatives requiring enriched media for optimal growth and plasmid maintenance.
Compared to the Miller formulation, LB Broth (Lennox) contains reduced sodium chloride (5.0 g/L vs 10.0 g/L), providing lower osmolarity (~170 mOsm/kg) that supports salt-sensitive strains, improved transformation efficiency, and stable plasmid propagation.
Principle of the Medium
| Tryptone (10.0 g/L) | Pancreatic digest of casein providing peptides, amino acids, nitrogen, and carbon for rapid bacterial growth |
| Yeast Extract (5.0 g/L) | Supplies B-vitamins, trace elements, nucleotides, coenzymes, and growth factors essential for E. coli K-12 |
| Sodium Chloride (5.0 g/L) | Maintains controlled osmotic balance — reduced (Lennox) vs 10 g/L (Miller) for salt-sensitive applications |
Composition (per litre)
| Component | CAS Number | Function | Amount |
|---|---|---|---|
| Tryptone | 73049-73-7 | Nitrogen, carbon, amino acids | 10.0 g |
| Yeast Extract | 8013-01-2 | Vitamins, growth factors | 5.0 g |
| Sodium Chloride | 7647-14-5 | Osmotic balance | 5.0 g |
| Final pH | 7.0 ± 0.2 at 25 °C | ||
Formulation Comparison
| Component | Lennox (AS-1269) | Miller |
|---|---|---|
| Tryptone | 10.0 g/L | 10.0 g/L |
| Yeast Extract | 5.0 g/L | 5.0 g/L |
| NaCl | 5.0 g/L | 10.0 g/L |
| Total solids | 20.0 g/L | 25.0 g/L |
| Best for | Salt-sensitive strains, transformation | Standard E. coli growth |
Applications
- Molecular Biology & Cloning: Routine E. coli cultivation, plasmid amplification, DNA transformation, protein expression (BL21)
- Transformation: Suitable for competent cell workflows and cloning applications where the reduced-salt Lennox formulation is preferred
- Salt-Sensitive Strains: E. coli K-12 derivatives, phage lambda infection studies, osmotic stress applications
- Antibiotic Selection: Compatible with ampicillin, kanamycin, chloramphenicol, and tetracycline supplementation
- Teaching & General Use: Standard molecular biology instruction and general bacterial propagation
Preparation
- Dissolve 20.0 g of dehydrated medium in 1 litre of demineralised or purified water.
- Mix or heat gently with agitation until fully dissolved.
- Verify pH and adjust to 7.0 ± 0.2 if required (1 M NaOH or 1 M HCl).
- Sterilise by autoclaving at 121 °C for 15 minutes.
- Cool to room temperature before use or inoculation.
- Add filter-sterilised antibiotics or supplements aseptically as required.
For LB Agar plates: add 15.0 g/L agar before autoclaving. Cool to 45–50 °C, add antibiotics aseptically, pour into sterile Petri dishes.
Common Antibiotic Supplements
| Antibiotic | Working Concentration | Application |
|---|---|---|
| Ampicillin | 100 µg/mL | Ampicillin-resistant plasmid selection |
| Kanamycin | 50 µg/mL | Kanamycin-resistant plasmid selection |
| Chloramphenicol | 25 µg/mL | Chloramphenicol-resistant selection |
| Tetracycline | 12.5 µg/mL | Tetracycline-resistant selection |
| Gentamicin | 10 µg/mL | Gentamicin-resistant selection |
Storage
| Dehydrated powder | 15–30 °C, tightly closed container |
| Prepared broth | 2–8 °C, use within 1 week |
| Protect from | Moisture, light, and extreme temperatures |
| Do not use if | Contamination, precipitation, turbidity, or pH shift observed |
For laboratory research, molecular biology, and educational use only. Not for medical, diagnostic, food, or veterinary applications. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
Product Identification
| Product Name | LB Broth (Lennox) |
| Catalogue Number | AS-1269 |
| HS Code | 3821.00.00 |
| Physical Form | Fine homogeneous powder |
| Colour (powder) | Light beige to cream |
| Physical state (prepared) | Clear to slightly turbid light amber liquid |
| Original Reference | Lennox E.S. (1955) Virology 1:190 |
| ATCC Medium | ATCC Medium 1065 |
Composition (per litre)
| Component | CAS Number | Function | Amount |
|---|---|---|---|
| Tryptone | 73049-73-7 | Nitrogen, carbon, amino acids, peptides | 10.0 g |
| Yeast Extract | 8013-01-2 | B-vitamins, growth factors, nucleotides | 5.0 g |
| Sodium Chloride | 7647-14-5 | Osmotic balance (reduced-salt Lennox) | 5.0 g |
Total: 20.0 g/L | pH: 7.0 ± 0.2 at 25 °C | Osmolarity: ~170 mOsm/kg
Physical & Chemical Specifications
| pH (prepared, 25 °C) | 7.0 ± 0.2 |
| Moisture (loss on drying) | ≤ 5.0% |
| Solubility | Readily and completely soluble in purified water |
| NaCl concentration | 5.0 g/L (Lennox — reduced vs Miller 10.0 g/L) |
| Osmolarity (approx.) | ~170 mOsm/kg (vs ~310 mOsm/kg for Miller) |
| Selectivity | Non-selective |
Performance Characteristics
| Organism | ATCC No. | Expected Result | Incubation |
|---|---|---|---|
| E. coli K-12 | 23716 | Good growth — turbid broth | 37 °C, shaking, 18–24 h |
| E. coli DH5α | N/A | Good growth — suitable for cloning | 37 °C, shaking, 12–16 h |
| E. coli BL21(DE3) | N/A | Good growth — protein expression host | 37 °C, shaking, 3–4 h to OD 0.6 |
| Salmonella typhimurium | 14028 | Good growth | 37 °C, shaking, 18–24 h |
Quality Standards
| Original Reference | Lennox E.S. (1955) Virology 1:190 |
| Standard References | Sambrook & Russell — Molecular Cloning; Current Protocols in Molecular Biology |
| ATCC Recommended | ATCC Medium 1065 |
| Country of Manufacture | Australia |
For laboratory and research use only. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au
GHS Classification: Not classified as hazardous (AU WHS Regulations 2011) | Signal Word: None | ADG: Not regulated
Section 1 — Identification
| Product Name | LB Broth (Lennox) |
| Catalogue Number | AS-1269 |
| Intended Use | Non-selective liquid culture medium — laboratory research, molecular biology, and educational use only |
| Supplier | AuSaMicS Pty Ltd | 36/25 Trafalgar Road, Epping VIC 3076 |
| ABN | 56 676 640 467 |
| Emergency Phone | +61 412 520 598 |
| support@ausamics.com |
Section 2 — Hazard Identification
Not classified as hazardous according to GHS criteria (AU WHS Regulations 2011). No hazard pictograms, signal word, or hazard statements required. Standard laboratory precautions apply.
Section 3 — Composition
| Component | CAS Number | Proportion | Classification |
|---|---|---|---|
| Tryptone | 73049-73-7 | ~50% | Not classified |
| Yeast Extract | 8013-01-2 | ~25% | Not classified |
| Sodium Chloride | 7647-14-5 | ~25% | Not classified |
Section 4 — First Aid
| Inhalation | Remove to fresh air. Seek medical advice if symptoms persist. |
| Skin Contact | Wash thoroughly with soap and water. |
| Eye Contact | Flush with water for at least 15 minutes. Seek medical advice if irritation persists. |
| Ingestion | Rinse mouth. Seek medical advice if large quantities ingested. |
Section 7 — Handling & Storage
| Handling | Standard laboratory precautions. Avoid dust generation. Wash hands after use. |
| Storage (powder) | 15–30 °C, tightly closed, dry, away from moisture and light |
| Storage (prepared) | 2–8 °C, use within 1 week |
Section 8 — Personal Protection
| Respiratory | Dust mask (P1) if handling in poorly ventilated area |
| Eye protection | Safety glasses recommended |
| Gloves | Disposable nitrile or latex laboratory gloves |
Section 13 — Disposal
Autoclave inoculated liquid cultures (121 °C, 30 min) before disposal. Dispose of unused dehydrated powder as general laboratory waste in accordance with local regulations.
Section 14 — Transport
Not classified as dangerous goods under ADG, IATA, or IMDG. No special transport requirements.
⬇ Download Full SDS — 16 Sections (PDF)Prepared in accordance with Australian WHS Regulations and GHS 7th Edition. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au | Version 1.0 — March 2026
Certificates of Analysis are batch-specific
Request / Find COA by Lot →
This tab does not show a generic or "typical" result set, because a COA belongs to one manufactured lot — not to the product in general. To obtain the actual Certificate of Analysis for the container you have (or are about to order), contact us with the product code and lot number printed on the label.
| Product Code | AS-1269 |
| Lot Number | Printed on your container label |
What's tested on every lot
| Test Parameter | Method |
|---|---|
| Appearance (powder & prepared) | Visual |
| pH (prepared, 25 °C) | Potentiometry |
| Moisture (loss on drying) | Loss on drying |
| Solubility | Visual / physical |
| Sterility (prepared medium) | Incubation, no growth |
| Growth promotion — E. coli K-12 ATCC 23716 | Inoculation & incubation |
| NaCl content (Lennox) | Titration / formulation check |
This table lists what each lot is tested against — it is not a result. Your lot's actual measured values are stated on its own Certificate of Analysis.
Authorisation
| Released by | Hassan Salimi |
| Title | Founder and Technical Director |
| Organisation | AuSaMicS Pty Ltd |
Need a batch-specific COA?
Contact us with your order number, product code and lot number and we will issue the corresponding COA within 1 business day.
support@ausamics.com | +61 412 520 598
Contact us with your order number, product code and lot number and we will issue the corresponding COA within 1 business day.
support@ausamics.com | +61 412 520 598
Certificates of Analysis are issued per manufactured lot by AuSaMicS Pty Ltd and are valid only for the batch identified on them. Results are not transferable between lots. AuSaMicS Pty Ltd | ABN 56 676 640 467 | www.ausamics.com.au