Lugol’s Solution 5% (Iodine/Potassium Iodide Solution)

Lugol’s Solution 5% (Iodine / Potassium Iodide Solution)

Aqueous Iodine Reagent for Microbiology, Analytical Chemistry & Research Applications

Product Identification

Chemical & Physical Information

Product Name: Lugol’s Solution 5%
Catalogue Number: AS-54002
Form: Aqueous solution

Iodine (I₂): 5 % w/v
Potassium Iodide (KI): 10 % w/v
Solvent: Purified water

Chemical Information

Component Details

Iodine
CAS Number: 7553-56-2

Potassium Iodide
CAS Number: 7681-11-0

Product Description

Lugol’s Solution 5% is a classical iodine-based aqueous reagent composed of elemental iodine solubilised with potassium iodide in purified water. The presence of potassium iodide increases iodine solubility and stability, producing a clear, chemically active solution suitable for a wide range of laboratory applications.

This formulation is widely used in microbiology, analytical chemistry, food testing, and educational laboratories, particularly as a mordant in Gram staining and as a qualitative reagent for starch detection. Ausamics Lugol’s Solution is manufactured under controlled conditions to ensure consistent concentration, stability, and reliable performance.

Principle of the Reagent

Functional Activity

Iodine forms polyiodide complexes in the presence of potassium iodide, increasing solubility in aqueous systems. In analytical applications, iodine interacts with polysaccharides such as starch to form a characteristic dark blue-black complex. In microbiology, Lugol’s Solution functions as a mordant by forming insoluble iodine–crystal violet complexes within bacterial cell walls during Gram staining.

Typical Applications

Microbiology & Laboratory Research

Mordant in Gram staining procedures
Differentiation of Gram-positive and Gram-negative bacteria

Analytical & Food Testing

Qualitative detection of starch in food and biological samples
General iodine-sensitive chemical reactions

Education & General Laboratory Use

Teaching demonstrations in microbiology and chemistry
Routine reagent use in research laboratories

Typical Characteristics

Physical Properties

Appearance: Brown to dark brown liquid
Odour: Characteristic iodine odour
Solubility: Fully soluble in water

Storage & Stability

Storage Conditions

Store in a tightly sealed amber container
Keep at room temperature in a cool, dry place
Protect from direct light and heat

Shelf Life: 24 months from date of manufacture when stored under recommended conditions

Intended Use

Regulatory Statement

For laboratory research and educational use only.

Not intended for human or veterinary use.
Not for diagnostic or therapeutic applications.
Users are responsible for compliance with local regulatory requirements.

Quality & Supply Assurance

Manufacturing & Documentation

Manufactured under controlled conditions
Supplied with batch-specific documentation upon request
Consistent formulation and performance
Technical support available from Ausamics Life Science

Customs & Trade Information

Classification

HS / AHECC Code: 3822.00.00
Prepared laboratory reagents