M9 Minimal Salts, 5X

M9 Minimal Salts 5X

Concentrated Defined Medium for Escherichia coli Cultivation in Molecular Biology

Product Identification

Product Name: M9 Minimal Salts 5X
Format: Concentrated aqueous solution
Concentration: 5X
pH: 7.4 ± 0.2 at 25 °C

Product Overview

M9 Minimal Salts 5X is a concentrated, chemically defined basal medium widely used for the cultivation of Escherichia coli in molecular biology and microbiology research. The formulation provides essential inorganic salts and a nitrogen source while deliberately excluding carbon and growth factors, allowing precise control of experimental conditions.

Supplied as a 5X concentrate, this product enables convenient preparation of working-strength M9 medium by dilution with purified water and supplementation with carbon sources (such as glucose), magnesium, calcium, vitamins, or amino acids as required. M9 Minimal Salts is commonly used in cloning workflows, plasmid amplification, recombinant protein expression, and physiological studies.

Ausamics M9 Minimal Salts 5X is manufactured to ensure reproducibility, buffering stability, and batch-to-batch consistency for research laboratories.

Key Features & Benefits

Defined composition for consistent and reproducible results
Buffered system suitable for controlled bacterial growth
5X concentrate for cost-efficient preparation of working medium
Flexible formulation easily supplemented for specialised applications
Supports E. coli growth in cloning, expression, and selective studies
Long shelf life under recommended storage conditions

Typical Applications

Molecular Biology & Microbiology

Cultivation of E. coli for cloning and plasmid production
Recombinant protein expression studies
Maintenance of defined growth conditions

Physiological & Functional Studies

Minimal medium for metabolism, motility, and stress-response assays
Controlled nutrient limitation experiments

Selective & Differential Media Preparation

Basal medium for preparation of selective or supplemented agar formulations
Custom minimal media development

Composition & Chemical Properties

Typical Composition (per litre of 5X solution)

Disodium hydrogen phosphate (Na₂HPO₄): 30.0 g
Potassium dihydrogen phosphate (KH₂PO₄): 15.0 g
Sodium chloride (NaCl): 2.5 g
Ammonium chloride (NH₄Cl): 5.0 g

pH: 7.4 ± 0.2 at 25 °C

(The final working medium is prepared by dilution to 1X and appropriate supplementation.)

Principle of the Medium

M9 Minimal Salts provides essential inorganic ions and a defined nitrogen source while omitting carbon and complex nutrients. This allows researchers to precisely control nutrient availability and study bacterial growth, metabolism, and gene expression under defined conditions.

Storage & Stability

Store at room temperature in a cool, dry environment
Protect from contamination
Ensure container is tightly closed when not in use

Intended Use

For laboratory research use only.

Not for medical, diagnostic, food, feed, or veterinary use.

Quality & Supply Assurance

Prepared under controlled conditions
Consistent batch-to-batch performance
Technical documentation available upon request
Supplied by Ausamics Life Science

Customs & Trade Information

HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms