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Modified Melin-Norkrans (MMN) Medium for Mycorrhizal Fungi Culture | Cat No. AS-1396
$45.00 AUD
Documentation
AuSaMicS Modified Melin-Norkrans (MMN) Medium (CAT: AS-1396) is a specialized microbiological culture medium formulated for the isolation, cultivation, and maintenance of ectomycorrhizal fungi and related mycorrhizal species. Its balanced formulation provides essential carbon, nitrogen, minerals, and vitamins required for robust mycelial development and symbiotic interaction studies. Widely used in forestry research, ecological mycology, and plant-fungal interaction investigations.
Product specifications
| Catalog number | AS-1396 |
| Product name | Modified Melin-Norkrans (MMN) Medium |
| Format | Dehydrated powder |
| Preparation quantity | 28–30 g per litre of purified water |
| Final pH (25°C) | 5.6 ± 0.2 |
| Sterilization | Autoclave at 121°C for 15 minutes |
| Grade | Laboratory / Research Grade |
| HS Code | 3821.00.00 — Prepared culture media for micro-organisms (AHECC) |
Key features
Optimized nutrient balance specifically for ectomycorrhizal and mycorrhizal fungi
Supports slow-growing and fastidious fungal species requiring extended incubation
Reproducible and standardized formulation for consistent experimental results
Versatile — suitable for both agar plates and broth preparation
Laboratory and research grade quality with full traceability
Composition (per litre)
| Ingredient | Amount | Function |
|---|---|---|
| Glucose | 10.00 g | Primary carbon and energy source |
| Malt extract | 3.00 g | Carbon source and growth stimulant |
| Ammonium nitrate (NH₄NO₃) | 0.25 g | Nitrogen source |
| Potassium dihydrogen phosphate (KH₂PO₄) | 0.50 g | Phosphate buffer and mineral |
| Magnesium sulphate (MgSO₄·7H₂O) | 0.15 g | Magnesium and sulfate source |
| Calcium chloride (CaCl₂·2H₂O) | 0.05 g | Calcium source |
| Ferric chloride (FeCl₃·6H₂O) | 0.01 g | Iron micronutrient |
| Thiamine HCl (Vitamin B₁) | 0.005 g | Essential growth vitamin |
| Agar (for solid medium) | 15.00 g | Solidifying agent |
| Purified water | q.s. to 1 L | Solvent |
Applications
Cultivation of ectomycorrhizal fungi
Isolation of slow-growing or fastidious mycorrhizal species
Forestry and ecological mycology research
Plant-fungal interaction studies
Inoculum production for symbiosis experiments
Mycorrhizal species maintenance and preservation
Preparation instructions
1
Suspend 28–30 g of dehydrated medium in 1 litre of purified water.
2
Heat with gentle agitation until fully dissolved.
3
Sterilize by autoclaving at 121°C for 15 minutes.
4
Cool to 45–50°C before pouring plates or dispensing into slants.
Incubation conditions
| Temperature | 20–25°C |
| Incubation time | 2–8 weeks (species dependent) |
Storage
Dehydrated medium: store at 15–30°C in a cool, dry place in a tightly sealed container
Prepared medium: store at 2–8°C, protected from light
Intended use
This product is intended for laboratory research use only by qualified professionals. Not for use in human or veterinary therapeutic applications, food, feed, or pharmaceutical manufacturing.
Quality & compliance
Manufactured and tested to laboratory research grade standards. Full batch-specific Certificate of Analysis (COA) and Safety Data Sheet (SDS) available upon request. For bulk quantities or contract pricing, contact sales@ausamics.com.
✓ Research grade
✓ COA available
✓ Ships from Australia
✓ 1–3 day delivery