MR-VP Broth (AS-1297) | Methyl Red & Voges-Proskauer Microbiology Media

$71.00 AUD

AuSaMicS Life Science • Differentiation Media

MR-VP Broth

Methyl Red & Voges-Proskauer Differential Medium

A critical diagnostic broth for the identification of coliform bacteria. Facilitates both the Methyl Red acid-production test and the Voges-Proskauer acetoin-production assay.

AS-1297 🧪 IMViC Standard ✓ ISO / APHA ⚗️ Glucose Pathway Test

Substrate: Glucose (Buffered) Incubation: 48h - 5 Days pH: 6.9 ± 0.2

Dual-Test Logic

MR Positive
Stable acid (Red color)

VP Positive
Acetoin production (Red/Pink)

Buffered Formula
Prevents false negatives

🔬 Analytical Applications

IMViC Series

Core component for the identification of E. coli, Klebsiella, and Enterobacter species.

Food & Water Safety

Distinguishes fecal coliforms from environmental coliforms in quality control samples.

🧪 Nutrient Formulation (per Liter)

Ingredient	Concentration	Function
Buffered Peptone	7.0 g	Nitrogen & Essential Nutrients
D-Glucose (Dextrose)	5.0 g	Fermentable Carbohydrate Substrate
Dipotassium Phosphate	5.0 g	Buffer (Maintains Initial pH)

📋 Technical Specs

Catalog No.	AS-1297
Final pH	6.9 ± 0.2
Yield	17.0 g / L
Prepared Color	Yellow, Clear

⚗️ Laboratory Prep

1 Suspend 17.0g in 1L distilled water.
2 Mix well and dispense into final tubes.
3 Autoclave at 121°C for 15 minutes.
4 For testing, split the broth after incubation for MR and VP assays.