Nalidixic Acid

Nalidixic Acid

Quinolone Antibiotic for Microbiology & Molecular Biology Research

Product Identification

Chemical Information

Product Name: Nalidixic Acid
Catalogue Number: AS-2015
CAS Number: 389-08-2
Molecular Formula: C₁₂H₁₂N₂O₃
Molecular Weight: 232.24 g/mol
Purity: ≥ 98 % (HPLC)
Form: White to off-white crystalline powder

Product Overview

Nalidixic Acid is a first-generation quinolone antibiotic widely used in microbiology and molecular biology research. It selectively inhibits bacterial DNA gyrase and topoisomerase II, enzymes essential for DNA replication, transcription, and chromosome segregation.

Although historically used as a therapeutic agent, Nalidixic Acid is now primarily employed as a research reagent, particularly in studies involving Gram-negative bacteria, antibiotic resistance mechanisms, plasmid stability, and selective media formulation.

Ausamics Nalidixic Acid is supplied at high purity (≥ 98 % HPLC) to ensure reproducibility and reliability in experimental workflows.

Mechanism of Action

Functional Activity

Inhibits DNA gyrase and topoisomerase II
Prevents DNA supercoiling and replication
Leads to inhibition of bacterial cell division

Solubility Characteristics

Solvent Compatibility

Soluble in ethanol
Soluble in methanol
Soluble in DMSO
Slightly soluble in water (enhanced with warming or pH adjustment)

Applications

Microbiology & Antibiotic Research

Antimicrobial susceptibility testing
Selective agent in microbiological media
Studies of quinolone resistance mechanisms

Molecular Biology & Genetics

DNA replication inhibition assays
Bacterial mutagenesis studies
Plasmid stability and maintenance research

Drug Discovery & Screening

Antibiotic mechanism and resistance screening models
Comparative studies of quinolone compounds

Key Features

Product Advantages

Selective inhibitor of bacterial DNA replication
High purity for consistent experimental results
Effective against a broad range of Gram-negative bacteria
Suitable for selective media and resistance studies

Storage & Stability

Storage Conditions

Store at 2–8 °C
Keep container tightly sealed
Protect from light and moisture

Stability

Stable for at least 2 years under recommended storage conditions.

Handling & Safety

Safety Information

Harmful if swallowed
May cause skin and eye irritation

Handling Precautions

Use appropriate personal protective equipment (PPE)
Handle in a well-ventilated area or fume hood

Refer to the Safety Data Sheet (SDS) for full hazard and handling information.

Intended Use & Regulatory Statement

Usage Disclaimer

For laboratory research use only.
Not intended for human or veterinary therapeutic use.
The purchaser is responsible for compliance with all applicable laws and regulations.