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Patent Blue V (Acid Blue 3) | Biological Dye & Food Colorant (E131) | Ausamics
$27.00 AUD
High-Purity Biological Stain & Colorant · Triphenylmethane Dye · E131
Patent Blue V (Acid Blue 3) — Calcium Salt
C.I. 42051 · CAS 3536-49-0 · EC 222-573-8 · Mol. Wt. 602.77 g/mol
E131 — EU Food Additive JECFA Evaluated EFSA Approved TGA Listed FSANZ Permitted Sentinel Lymph Node Mapping
AuSaMicS
Life Science™
Cat No.
ASD-628
CAS No.
3536-49-0
Mol. Weight
602.77 g/mol
λmax
~640 nm
Storage
+20 °C, dark, dry
Overview
Patent Blue V — Acid Blue 3 Triphenylmethane dye · λmax ~640 nm · Intense blue-green colour
Patent Blue V (Acid Blue 3, C.I. 42051) is a synthetic triphenylmethane dye of high commercial and scientific significance. The calcium salt form (CAS 3536-49-0) is the predominant pharmaceutical and food-grade variant, characterised by its intense blue-green colour, high aqueous solubility (up to 20.98 g/L at 20 °C), and stability across a broad pH range (3–10).
The dye absorbs strongly at ~640 nm, producing vivid blue colouration readily visualisable in tissue sections, lymphatic vessels, and solution-based assays. In Europe it is authorised as food additive E131 (Food Blue 5) under Regulation (EC) No. 1333/2008, and is approved for sentinel lymph node mapping in surgical oncology by TGA and EMA. AuSaMicS supplies a high-purity, batch-traceable grade suitable for regulated scientific and food applications.
Chemical Identity
| Chemical Name | Patent Blue V calcium salt |
| IUPAC Name | Ethanaminium, N-[4-[4-(diethylamino)phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-, inner salt, calcium salt (2:1) |
| Molecular Formula | C₂₇H₃₄CaN₂O₇S₂ |
| Molecular Weight | 602.77 g/mol |
| CAS No. | 3536-49-0 |
| C.I. No. | 42051 (Acid Blue 3) |
| EC No. | 222-573-8 |
| Absorption Maximum | ~640 nm (aqueous, neutral pH) |
| Solubility | Up to 20.98 g/L in water at 20 °C (highly soluble) |
| Storage | +20 °C, protected from light and moisture; hygroscopic — keep tightly closed |
Applications
Biological Staining
Histology and cytology tissue visualisation; lymphatic vessel staining; chromogenic media component and bacterial identification assays.
Medical Diagnostics
Approved for sentinel lymph node mapping in breast cancer surgery (TGA · EMA). Intraoperative lymphatic visualisation.
Food Colouring (E131)
Authorised as E131 (Food Blue 5) in Europe for gelatin desserts, sweets, beverages, and confectionery. FSANZ permitted in Australia/NZ.
Regulatory Status
E131 — EU Food Law
Authorised under Regulation (EC) No. 1333/2008 for specified food categories
JECFA
Evaluated by FAO/WHO Joint Expert Committee; ADI established
EFSA
Re-evaluated under EU programme; authorised at current approved uses
TGA (Australia)
Approved pharmaceutical colourant and diagnostic agent (sentinel lymph node)
FSANZ
Permitted food colour under Australian and New Zealand Food Standards
AICS
Listed on Australian Inventory of Chemical Substances
Quality Highlights
High-Purity Batch-Traceable CAS Verified COA Available Vegan Gluten Free Non-GMO
Packaging & Storage
| Pack Sizes | 10 g · 50 g · 100 g |
| Storage Temperature | +20 °C (room temperature); protect from light and moisture |
| Container | Tightly closed, original container; amber glass or light-resistant vessel preferred |
| Hygroscopicity | Hygroscopic — store with desiccant; reseal immediately after use |
| Shelf Life | See retest date on label; typically 3 years from manufacture under correct conditions |
AuSaMicS Pty Ltd (ABN 56 676 640 467) warrants this product meets stated specifications at time of manufacture. Supplied for laboratory, diagnostic, and permitted food/feed applications. Not for unauthorized pharmaceutical, therapeutic, or injectable use. · 31 Longview CT, Thomastown VIC 3074, Australia · support@ausamics.com · +61 412 520 598 · www.ausamics.com.au
Product Identification
| Product Name | Patent Blue V calcium salt |
| Cat No. | ASD-628 |
| CAS No. | 3536-49-0 |
| C.I. No. | 42051 |
| EC No. | 222-573-8 |
| Lot No. | PBV260301 |
| Grade | High-Purity Biological Stain / Colorant |
Physical Properties
| Appearance | Dark blue to green crystalline powder; hygroscopic |
| Molecular Formula | C₂₇H₃₄CaN₂O₇S₂ |
| Molecular Weight | 602.77 g/mol |
| λmax (aq., pH 7) | ~640 nm |
| Solubility (water) | Up to 20.98 g/L at 20 °C |
| pH (1% aq. soln.) | ~4.0 – 6.0 |
| Hygroscopic | Yes — use desiccant storage |
Spectroscopic Data
| Absorption Maximum (λmax) | ~640 nm in aqueous solution (neutral pH) |
| Secondary Absorption | ~420 nm (minor shoulder) |
| Molar Absorptivity (ε) | ~35,000–40,000 L·mol⁻¹·cm⁻¹ at λmax |
| Beer-Lambert Range | Linear up to ~50 mg/L for standard colorimetric use |
| Fluorescence | Non-fluorescent under standard conditions |
| Recommended Solvent | Purified water or phosphate-buffered saline (PBS) |
Preparation & Use Guidelines
| Stock Solution | Dissolve 10–50 mg in 1 mL purified water. Vortex gently until fully dissolved. Filter through 0.22 µm if required for injectable or cell culture applications. |
| Working Dilution | 0.1–5 mg/mL for biological staining; consult specific protocol for application |
| pH Adjustment | Aqueous solution pH ~4–6; adjust with PBS for physiological applications |
| Solution Stability | Stable for several weeks at RT, protected from light; freeze at −20 °C for long-term storage |
| Incompatibilities | Strong oxidising agents (bleach, permanganate) decolourise the dye; strong acids/bases alter colour |
Quality Control Specifications
| Parameter | Specification | Method |
|---|---|---|
| Appearance | Dark blue to green crystalline powder; no visible impurities | Visual inspection |
| Identity (UV-Vis λmax) | 635–645 nm (aqueous, pH 7) | UV-Vis spectrophotometry |
| Dye Content | High purity — see COA for lot-specific value | Spectrophotometric assay |
| Absorbance Ratio A640/A420 | > 3.5 (purity indicator) | UV-Vis dual wavelength |
| Solubility (20 mg/mL) | Complete dissolution in purified water | Solution test |
| Loss on Drying | ≤ 10.0% w/w | 105 °C / 2 h (gravimetric) |
| Heavy Metals (Pb equiv.) | ≤ 20 ppm | ICP-MS |
| Arsenic | ≤ 3 ppm | ICP-MS |
AuSaMicS Pty Ltd (ABN 56 676 640 467) · 31 Longview CT, Thomastown VIC 3074, Australia · support@ausamics.com · +61 412 520 598 · www.ausamics.com.au
Safety Data Sheet — GHS Format (Australian WHS Regulations 2017)
Issue Date: March 2026 · Rev: 1.0 · Prepared in accordance with WHS Regulations and GHS 7th Revision.
Issue Date: March 2026 · Rev: 1.0 · Prepared in accordance with WHS Regulations and GHS 7th Revision.
Section 1 — Identification
| Product Name | Patent Blue V (Acid Blue 3) — Calcium Salt (C.I. 42051) |
| Other Names | Acid Blue 3; E131; Food Blue 5; Sulphan Blue; C.I. 42051 |
| Catalog No. | ASD-628 · CAS: 3536-49-0 |
| Intended Use | Biological staining, food colouring (E131), diagnostic colorant — laboratory and permitted applications |
| Supplier | AuSaMicS Pty Ltd, 31 Longview CT, Thomastown VIC 3074, Australia |
| Phone / Emergency | +61 412 520 598 · Poisons Centre: 13 11 26 (24h) |
| Email / Web | support@ausamics.com · www.ausamics.com.au |
Section 2 — Hazard Identification
| GHS Classification | Not classified as hazardous under GHS / WHS Regulations 2017 at supplied concentration. Precautionary irritant classification applies to dust/concentrated solution. |
| Signal Word | Warning (precautionary) |
| Hazard Statements | H315: Causes skin irritation · H319: Causes serious eye irritation · H335: May cause respiratory irritation (dust) |
| Precautionary | P260 · P264 · P280 · P305+P351+P338 · P312 · P501 |
| Other Hazards | Intensely coloured dye — stains skin, clothing, and surfaces. Hygroscopic. Anaphylaxis associated with injectable form (clinical) — not applicable to this laboratory/food-grade supply. |
Section 3 — Composition
| Component | CAS No. | Proportion (w/w) | GHS Classification |
|---|---|---|---|
| Patent Blue V calcium salt | 3536-49-0 | ≥ 85% | Not classified at supplied concentration |
| Water of crystallisation / moisture | 7732-18-5 | ≤ 10% | Not classified |
| Minor manufacturing residues | — | < 5% | Not classified |
Sections 4–8 — First Aid, Fire, Spill, Handling, PPE
| Inhalation | Remove to fresh air. If respiratory irritation from dust persists, seek medical advice. |
| Skin Contact | Wash with soap and water. Dye stains skin blue/green transiently (fades within hours–days). Seek advice if irritation persists. |
| Eye Contact | Rinse immediately with copious water ≥ 15 min, holding eyelids open. Seek medical attention if irritation persists. Conjunctival staining is transient. |
| Ingestion | Rinse mouth. Seek medical advice. Low acute oral toxicity (EU food additive). May cause transient blue colouration of urine/faeces. |
| Flash Point | Not applicable (non-flammable crystalline solid) |
| Extinguishing Media | CO², dry chemical, or water spray |
| Spill (dry) | Sweep or vacuum carefully. Avoid airborne dust. Collect in sealed container. Dispose per Section 13. |
| Spill (solution) | Absorb with inert material (sand, vermiculite). Collect and seal. Rinse area — dye staining may persist on porous surfaces. |
| Engineering Controls | Local exhaust ventilation when weighing dry powder |
| Respiratory PPE | P2 dust mask when generating dust from powder |
| Hand Protection | Nitrile or latex gloves — dye stains skin intensely |
| Eye Protection | Safety glasses; goggles if splash risk from concentrated solutions |
| Body Protection | Lab coat, closed footwear; dye-resistant apron for large volumes |
Sections 9–16 — Properties, Stability, Toxicology, Disposal, Regulatory
| Physical State | Solid (crystalline powder); dark blue to green |
| Absorption Maximum | ~640 nm (aqueous) |
| Vapour Pressure | Negligible |
| Stability | Stable at +20 °C, dry, light-protected. Oxidising agents (bleach) decolourise. |
| Acute Oral Toxicity | Low — authorised EU food additive (E131); ADI established by JECFA |
| Skin / Eye Irritation | H315 / H319 — mild irritant; intense staining of skin/conjunctiva (transient) |
| Sensitisation (injectable) | Anaphylaxis reported with clinical injectable form (Sulphan Blue) — not applicable to this supply grade |
| Mutagenicity | Not classified (AMES: no clear mutagenicity per JECFA monograph) |
| Carcinogenicity | Not classified; IARC not evaluated |
| Aquatic Toxicity | Visible blue colour at mg/L levels; prevent discharge to waterways |
| Biodegradability | Limited direct biodegradability of synthetic dye; decolourise with bleach before drain disposal |
| Waste Disposal | Dilute solutions: decolourise with NaOCl before drain disposal. Solid: chemical waste contractor. Decolourise: add dilute bleach to aqueous dye waste. |
| Transport | Not regulated as dangerous goods (ADG / IATA / IMDG) |
| AICS (Australia) | Listed on Australian Inventory of Chemical Substances |
| SUSMP Schedule | Not scheduled for laboratory/food use; injectable pharmaceutical form — consult TGA |
| EU Food Law (E131) | Authorised under Regulation (EC) No. 1333/2008 |
| SDS Prepared By | AuSaMicS Pty Ltd — Technical & Quality Department · March 2026 · Rev 1.0 |
AuSaMicS Pty Ltd (ABN 56 676 640 467) · 31 Longview CT, Thomastown VIC 3074, Australia · support@ausamics.com · +61 412 520 598 · www.ausamics.com.au
This SDS is prepared in accordance with Australian WHS Regulations and GHS (7th Rev.). For laboratory and permitted food/feed use only.
This SDS is prepared in accordance with Australian WHS Regulations and GHS (7th Rev.). For laboratory and permitted food/feed use only.
Product Details
| Product Name | Patent Blue V (Acid Blue 3) |
| Catalog No. | ASD-628 |
| CAS No. | 3536-49-0 |
| C.I. No. | 42051 |
| Lot No. | PBV260301 |
| Mfg. Date | March 2026 |
| Retest Date | March 2028 |
| Grade | High-Purity Biological Stain / Colorant |
Document Information
| COA No. | COA-ASD-628-L001 |
| Issue Date | March 2026 |
| Issued By | AuSaMicS Quality Control |
| Test Ref. | In-house / Ph. Eur. / JECFA |
Test Results & Analytical Data
| Test Parameter | Specification | Method | Result | Status |
|---|---|---|---|---|
| Appearance | Dark blue to green crystalline powder; no visible impurities or clumping | Visual inspection | Conforms | PASS |
| Identity — UV-Vis (λmax) | 635–645 nm (aqueous, pH 7 ± 0.5) | UV-Vis spectrophotometry | 641 nm | PASS |
| Dye Content (spectrophotometric) | High purity grade — see lot-specific value | Spectrophotometric assay | See lot value | PASS |
| Absorbance Ratio A640/A420 | > 3.5 (low intermediate impurity) | UV-Vis dual wavelength | 4.1 | PASS |
| Solubility (20 mg/mL, water) | Complete dissolution within 5 min at 20 °C | Solubility test | Complete | PASS |
| Loss on Drying | ≤ 10.0% w/w (hygroscopic material) | 105 °C / 2 h (gravimetric) | 6.2% | PASS |
| pH (1% aqueous solution) | 4.0 – 6.5 | Potentiometry (ISO 10523) | 5.1 | PASS |
| Heavy Metals (Pb equiv.) | ≤ 20 ppm | ICP-MS | < 5 ppm | PASS |
| Arsenic | ≤ 3 ppm | ICP-MS | < 1 ppm | PASS |
| Microbiological Quality | TVC ≤ 1000 CFU/g; pathogens absent | USP <61>/<62> | Conforms | PASS |
| CAS / C.I. Identity Verification | CAS 3536-49-0; C.I. 42051 confirmed | Spectral + literature comparison | Confirmed | CONFORM |
Conclusion: Lot PBV260301 fully meets all release specifications for Patent Blue V calcium salt (CAS 3536-49-0, C.I. 42051). This lot is RELEASED for use in biological staining, food colouring (E131), and permitted diagnostic applications.
Quality Control Analyst
AuSaMicS Quality Control Department
Date: March 2026 | Signature: _______________
Quality Manager
AuSaMicS Pty Ltd
Date: March 2026 | Signature: _______________
AuSaMicS Pty Ltd (ABN 56 676 640 467) warrants this product meets specifications at time of manufacture and release. Supplied for laboratory, diagnostic, and permitted food/feed applications only. AuSaMicS Pty Ltd shall not be held liable for any damages resulting from use outside the intended application. · 31 Longview CT, Thomastown VIC 3074, Australia · support@ausamics.com · +61 412 520 598 · www.ausamics.com.au
AuSaMicS Pty Ltd · ABN 56 676 640 467 · 31 Longview CT, Thomastown VIC 3074, Australia · support@ausamics.com · +61 412 520 598 · www.ausamics.com.au