Pikovskaya Broth: Selective Medium for Phosphate-Solubilising Microorganisms; Cat: AS-1442
$145.00 AUD
Documentation
AuSaMicS Life Science™ · Dehydrated Culture Media · Soil & Agricultural Microbiology
Pikovskaya Broth
Selective Medium for Phosphate-Solubilising Microorganisms | Cat: AS-1442
A selective liquid medium supplying insoluble tricalcium phosphate as the sole phosphorus source, used for detecting and cultivating phosphate-solubilising soil bacteria and fungi. A standard screening tool in agricultural microbiology and biofertiliser research. Australian stock held in Melbourne — same-day dispatch with full COA documentation.
AS-1442 In Stock Australia Same-Day Dispatch Pikovskaya 1948 Formula
Quick Specs
CatalogueAS-1442
Dissolution16.3 g/L
Final pH7.0 ± 0.2
Sterilisation121°C / 15 min
ReferencePikovskaya, 1948
Overview
Pikovskaya Broth is a modification of Pikovskaya's original agar medium (Pikovskaya, 1948), further adapted by Sundara Rao and Sinha for cultivating phosphate-solubilising soil microorganisms. Many naturally occurring soil fungi and bacteria are phosphate solubilisers, playing an important role in maintaining phosphorus balance for crop plants.
The medium supplies insoluble tricalcium phosphate as the sole phosphate source. Organisms capable of solubilising this insoluble phosphate produce a visible clearing (or, in liquid culture, a reduction in the flocculent precipitate) as calcium phosphate is converted to soluble forms.
Mode of Action
Phosphate-solubilising microorganisms secrete organic acids and/or acid phosphatases that dissolve or hydrolyse the tricalcium phosphate, releasing soluble phosphate. Dextrose provides an energy source for acid production; yeast extract and ammonium sulfate supply nitrogen and growth factors; trace manganese and ferrous sulfate support enzymatic function. Solubilisation is detected as a zone of clearing around colonies on solid medium, or reduced turbidity/precipitate in broth.
Applications
| Application | Notes |
|---|---|
| Screening of phosphate-solubilising bacteria (PSB) and fungi | Standard method for isolating candidate biofertiliser organisms from soil, rhizosphere, and compost samples |
| Agricultural microbiology research | Study of phosphorus cycling and plant growth-promoting rhizobacteria (PGPR) |
| Biofertiliser development | Pre-screening of candidate strains prior to field trial or formulation work |
Intended use: For laboratory and agricultural research use only. Not intended for therapeutic, diagnostic, or food-safety testing applications.
Technical Data Sheet Summary
Formula — Per Litre
| Component | g/L | Role |
|---|---|---|
| Dextrose (Glucose) | 10.000 | Energy source |
| Calcium Phosphate | 5.000 | Insoluble phosphate source |
| Yeast Extract | 0.500 | Nitrogen, growth factors |
| Ammonium Sulfate | 0.500 | Nitrogen source |
| Potassium Chloride | 0.200 | K⁺ ion source |
| Magnesium Sulfate | 0.100 | Mg²⁺ ion source |
| Manganese Sulfate | 0.0001 | Trace mineral |
| Ferrous Sulfate | 0.0001 | Trace mineral |
Total: 16.3 g/L. Final pH 7.0 ± 0.2 at 25°C.
Preparation
| Dissolution | Suspend 16.3 g in 1 litre of distilled/purified water. Heat if necessary to dissolve completely. |
| Sterilisation | Autoclave at 121°C for 15 minutes. |
| Detection | Subculture or spot-inoculate onto Pikovskaya Agar to visualise phosphate solubilisation as a clear halo zone. |
Storage
| Dehydrated Powder | Store below 30°C, tightly closed, protected from moisture and light. Hygroscopic. |
| Prepared Broth | 2–8°C. Discard if contaminated or discoloured. |
Safety Data Sheet Summary
Full 16-section SDS prepared in accordance with the GHS and Australian WHS Regulations (Safe Work Australia).
| GHS Classification | Not classified as a hazardous substance or mixture |
| Signal Word / Pictograms | None required |
| Biosafety Note | Microbiological growth medium — treat inoculated cultures as potentially containing viable soil microorganisms; handle per AS/NZS 2243 series |
| Transport | Not classified as Dangerous Goods (ADG, IATA, IMDG); no UN Number applicable |
Full 16-section GHS-compliant SDS is available as a downloadable document — contact support@ausamics.com quoting catalogue number AS-1442.
Certificate of Analysis
Each batch of Pikovskaya Broth (AS-1442) is released with a batch-specific Certificate of Analysis.
| Test Parameter | Specification |
|---|---|
| Dehydrated Appearance | White to light yellow, homogeneous, free-flowing powder |
| Prepared Appearance | Whitish, opaque solution with flocculent precipitate |
| pH (prepared, 25°C) | 7.0 ± 0.2 |
| Productivity Test | Bacillus megaterium (phosphate solubiliser) — growth with halo/precipitate reduction |
Batch-specific COA is issued with every shipment. Contact support@ausamics.com quoting catalogue number AS-1442.