Pyruvate Broth

Pyruvate Broth

$45.00 AUD
Request a Quote
EMPTY DOM REMOVE PROTECTOR
AuSaMicS Life Science • Differential Media

Pyruvate Broth

Confirmatory Test for Enterococcus spp.

Specific liquid medium designed to detect pyruvate utilization. Essential for differentiating Enterococcus species from group D streptococci through clear-cut acid production and color change.

AS-1335 🧪 Differential ✓ FDA-BAM Compliant 🥼 Enterococci Control
Target: Enterococcus spp. Indicator: Bromocresol Purple Standard: FDA-BAM Chapter 5

Differentiation Specialist

Reliable Confirmation - Single-tube test
Clinical Grade - Identification algorithms
Clear Interpretation - Purple to Yellow
Fast Supply - Ships within 24h
Validated - FDA-BAM & National standards
High Specificity
Sodium Pyruvate Base

🧪 Complete Formulation & Nutrient Profile

📋 Ingredient Composition (per Liter)

Ingredient Concentration Primary Function
Proteose Peptone / Tryptone 10.0 g Nitrogen & Amino Acid source
Yeast Extract 5.0 g B-complex vitamins & growth factors
Sodium Pyruvate 10.0 - 15.0 g Sole fermentable carbon source
Sodium Chloride 5.0 g Osmotic equilibrium
Bromocresol Purple 0.02 g pH Indicator (Purple to Yellow)

⚗️ Specialized Detection System

Carbon Specificity
Pyruvate is the restricted energy source for acid production
Indicator Precision
Bromocresol purple offers clear visual distinction at pH shift
Nutrient Balance
Yeast extract ensures growth of fastidious streptococci
Reliable Differentiation: Effectively separates Enterococcus (Positive) from S. bovis/S. equinus (Negative), critical for clinical diagnostics.

Enterococci Confirmatory Media Portfolio

Medium Target Reaction Specificity Form Best Application
Pyruvate Broth Pyruvate Utilization High (Enterococcus) Broth Speciation of Group D Strep
Bile Esculin Azide Esculin Hydrolysis Broad (Group D) Agar Primary isolation/screening
6.5% NaCl Broth Salt Tolerance High (Enterococcus) Broth Differential confirmatory test

Strategic Selection for Enterococci Confirmation

✅ Pyruvate Broth Advantages

  • ✓ Highly Specific: Reliable speciation
  • ✓ Clear-cut Results: Sharp color change
  • ✓ Standardized: Follows FDA-BAM protocols
  • ✓ Versatile: Clinical and food microbiology

⚠️ Method Considerations

  • ▲ Rare Isolates: Leuconostoc may show weak positive
  • ▲ Incubation: Requires up to 48 hours for confirmation
  • ▲ Light Sensitivity: Indicator must be protected

📋 Technical Specifications

Catalogue Number AS-1335
Final pH 6.9 ± 0.1 (at 25°C)
Control Indicator Bromocresol Purple
Sterilization 121°C, 15 minutes
Shelf Life (Prep) 3 months (2-8°C)

🔍 Interpretation Guide

  • 🟡 Positive: Yellow color (Acid produced) - Enterococcus spp.
  • 🟣 Negative: Remains Purple - S. bovis, S. equinus
  • *Read at 24h and 48h for final confirmation.

⚗️ Preparation Protocol

  1. 1 Suspend 30.0 - 35.0g in 1L purified water
  2. 2 Dispense 4-5 mL into small tubes
  3. 3 Autoclave at 121°C for 15 min
  4. 4 Incubate at 35-37°C for 18-48h

★★★★★
★★★★★
★★★★★
★★★★★

CUSTOMERS ALSO BOUGHT