Sabouraud Dextrose Broth - SDB

Sabouraud Dextrose Broth (SDB)

Enriched Fungal Broth for Cultivation of Yeasts and Moulds

Product Identification

Chemical & Physical Information

Product Name: Sabouraud Dextrose Broth
Catalogue Number: AS-1343
Medium Type: Enriched liquid culture medium
Form: Dehydrated powder

Product Description

Sabouraud Dextrose Broth (SDB) is a general-purpose, enriched fungal liquid medium formulated for the cultivation, maintenance, and enrichment of yeasts and filamentous moulds. The medium contains a high concentration of dextrose and is adjusted to a slightly acidic pH, creating conditions that favour fungal growth while inhibiting many bacterial contaminants.

SDB is widely used in clinical microbiology, pharmaceutical and cosmetic quality control, food microbiology, and mycological research. It is also specified in pharmacopeial sterility and microbial limit testing, where reliable recovery of fungi is required.

Ausamics Sabouraud Dextrose Broth is supplied as a high-quality dehydrated powder to ensure clarity, reproducibility, and consistent fungal recovery.

Principle of the Medium

Functional Components

Peptones (casein / animal origin): Provide nitrogen, amino acids, and peptides
Dextrose: High-level carbohydrate source to promote fungal metabolism
Acidic pH: Suppresses many bacterial species while supporting fungal growth

Typical Characteristics

Medium Properties

Appearance (dehydrated): Light beige powder
Appearance (prepared): Clear to light yellow broth
pH (prepared medium): 5.6 ± 0.2 at 25 °C
Selectivity: Favourable for fungi
Differential: No

Composition

Standard Formula (per litre)

Peptones (casein / animal): 10.0 g
Dextrose: 40.0 g

Typical Applications

Mycology & Clinical Microbiology

Cultivation and enrichment of yeasts and moulds
Recovery of stressed or slow-growing fungal isolates

Pharmaceutical & Cosmetic Quality Control

Sterility testing and microbial limit testing
USP ⟨61⟩ / ⟨62⟩
European Pharmacopoeia 2.6.12
Japanese Pharmacopoeia

Research & Identification

Preparation of fungal suspensions for identification tests
Sporulation and morphological studies

Preparation Instructions

Medium Preparation

Suspend 50 g of dehydrated medium in 1 L purified water
Heat with agitation until fully dissolved
Autoclave at 121 °C for 15 minutes
Cool to room temperature
Use as a clear, light-yellow broth

Incubation Conditions

Recommended Conditions

Incubation temperature: 25–30 °C
Incubation time: 24–96 hours
Extended incubation: Up to 14 days for slow-growing fungi

Storage & Stability

Storage Conditions

Prepared broth: Store at 2–8 °C in a tightly closed container
Recommended use period: 1–3 months
Discard if contamination, turbidity, or pH drift is observed

Intended Use

Regulatory Statement

For laboratory, pharmaceutical, cosmetic, food testing, and research use only.

Not intended for medical treatment, diagnosis, or food consumption.

Quality & Supply Assurance

Manufacturing & Documentation

Manufactured under controlled conditions
Consistent batch-to-batch performance
Certificate of Analysis (COA) available upon request
Safety Data Sheet (SDS) available
Technical support provided by Ausamics Life Science

Reference

Standards & Literature

USP ⟨61⟩ / ⟨62⟩
European Pharmacopoeia 2.6.12
ISO 16212
Sabouraud, R. (1892)

Customs & Trade Information

Classification

HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms