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Salmon-Caprylate (6-Chloro-3-indolyl caprylate) | ASE-909 | C8 Esterase Substrate

Salmon-Caprylate (6-Chloro-3-indolyl caprylate) | ASE-909 | C8 Esterase Substrate

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AuSaMicS Life Science • Enzyme Substrates

Salmon-Caprylate

6-Chloro-3-indolyl caprylate (C8 Esterase)

Precision chromogenic substrate for C8 esterase detection. Essential for differential identification of Salmonella, Pseudomonas, and fungal species in clinical diagnostics and food safety applications.

CAS: 159954-35-5 ASE-909 ✓ Australian Stock 🔴 C8 Esterase
🚀 Same-Day Dispatch: Australian Stock 🧪 Diagnostic Grade: Clinical applications ❄️ Stable: -20°C Storage, Light Protected

🏆 Diagnostic Specialist

🦠 Salmonella Detection - Food safety standard
🔬 Clinical Grade - Diagnostic applications
📋 Complete Package - COA + SDS included
Fast Supply - Ships within 24h
📞 Expert Support - Formulation guidance
Food Safety Critical
Essential for pathogen detection

🔬 C8 Esterase Detection Mechanism

Salmon-Caprylate undergoes esterase-catalyzed hydrolysis at the C8 (caprylate) ester bond, releasing 6-chloro-3-indoxyl which rapidly oxidizes to form a distinctive salmon-red precipitate:

6-Chloro-3-indolyl caprylate + H₂O
↓ (C8 Esterase/Lipase)
6-Chloro-3-indoxyl + Caprylic acid
↓ (Spontaneous oxidation)
Salmon-Red Indigoid Precipitate
Specificity: Detects C8 esterase activity - diagnostic marker for Salmonella and select Pseudomonas species

🧬 Clinical & Food Safety Applications

🦠 Salmonella Identification

Differential detection in food matrices, clinical samples, and environmental monitoring programs.

🧪 Pseudomonas Profiling

Characterization of P. aeruginosa and related species based on C8 esterase profiles.

Industry Applications:

  • Food Industry: Salmonella screening in poultry, eggs, produce
  • Clinical Labs: Rapid pathogen identification
  • Water Testing: Environmental contamination monitoring
  • Pharmaceutical QC: Raw material contamination testing
  • Research: Microbial enzyme characterization
  • Veterinary: Animal health diagnostics

📊 Comprehensive Chromogenic Substrate Comparison

Substrate Target Enzyme Signal Color Key Organisms Applications
X-Gal β-Galactosidase 🔵 Blue E. coli, Lactobacillus Blue-white screening, coliform detection
X-Gluc β-Glucuronidase 🔵 Blue E. coli (83%) E. coli confirmation, GUS reporter
Salmon-Caprylate C8 Esterase 🔴 Salmon-Red Salmonella, Pseudomonas Food safety, clinical diagnostics
Magenta-Gal β-Galactosidase 🟣 Purple E. coli, Enterobacteria Alternative screening, visual contrast
ONPG β-Galactosidase 🟡 Yellow Various bacteria Quantitative assays, biochemical tests
X-Phos Alkaline phosphatase 🔵 Blue E. coli, Salmonella Phosphatase detection, ELISA
MUG β-Glucuronidase 💙 Fluorescent Blue E. coli detection Fluorogenic detection, water testing

🧪 Chromogenic Media Formulation Guide

Application Field Substrate Concentration Typical Incubation Expected Result
Food Safety (Salmonella) 50-100 μg/mL 35°C, 18-24h Red colonies
Clinical Diagnostics 75-150 μg/mL 37°C, 4-18h Rapid red development
Environmental Testing 25-50 μg/mL 30°C, 24-48h Variable intensity
Research Applications 10-200 μg/mL 25-37°C, 1-72h Dose-dependent
Quality Control 100 μg/mL (standard) 35°C, 24h Consistent red signal

💡 Formulation & Usage Guidelines

Stock Preparation:
Dissolve in DMSO (10-50 mg/mL)
Working Concentration:
50-100 μg/mL in media
Storage:
-20°C, protect from light & moisture
Stability:
Stock stable 1 year, media 48h at 4°C

📋 Technical Specifications

Chemical Name 6-Chloro-3-indolyl caprylate
CAS Number 159954-35-5
Formula C₁₆H₂₀ClNO₂
Molecular Weight 293.79 g/mol
Purity ≥98% (HPLC)
Appearance White to pale yellow powder
Solubility DMSO, DMF, limited in ethanol
Storage -20°C, protect from light
HS Code 2933.99.00

📝 Alternative Names

Salmon-Caprylate
Salmon-Octanoate
6-Chloro-3-indolyl octanoate
6-Chloroindolyl caprylate
C8 Esterase substrate

⚖️ Regulatory & Standards

  • ISO 11290 - Salmonella detection methods
  • AOAC Guidelines - Food microbiology
  • FDA BAM - Bacteriological Analytical Manual
  • NATA Approved - Australian laboratory standards

✅ Quality Assurance

  • HPLC Purity: ≥98% verified
  • Enzymatic Activity: Functional testing
  • Microbial Testing: Sterility confirmed
  • Heavy Metals: <10 ppm total
  • Storage Stability: Validated conditions

🧬 Complete Diagnostic Media Kit

X-Gluc

E. coli β-glucuronidase detection

View Product

Compass Salmonella Agar

Complete selective medium

View Product

Pseudomonas Agar Base

Selective isolation medium

View Product

C8 Esterase Test Kit

Ready-to-use diagnostic kit

View Product

Need Media Formulation Support?

Our microbiologists can help optimize your Salmonella detection protocols

For laboratory, research, and clinical use only. Not for human consumption or medicinal use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • Full documentation included

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