TMPPD Powder (N,N,N′,N′-Tetramethyl-p-phenylenediamine Dihydrochloride)
TMPPD Powder (N,N,N′,N′-Tetramethyl-p-phenylenediamine Dihydrochloride)
Cytochrome Oxidase Reagent | Wurster’s Reagent
Product Identification
Chemical Information
Product Name: TMPPD Powder
Catalogue Number: ASC-1010
Chemical Name: N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride
Synonyms: TMPD, TMPDA, TMPPD, Wurster’s Reagent
CAS Number: 637-01-4
Molecular Formula: C₁₀H₁₆N₂ · 2HCl
Molecular Weight: 237.17 g/mol
Form: Crystalline powder
Purity: ≥ 97 %
Product Description
TMPPD Powder, also known as Wurster’s reagent, is a high-purity, readily oxidisable aromatic diamine widely used in microbiological diagnostics and biochemical research.
It functions as a rapid electron donor and colour-forming reagent, making it particularly valuable in cytochrome oxidase testing and enzyme-based detection systems. Upon oxidation, TMPPD produces a characteristic blue-purple colour, enabling fast and reliable visual interpretation in laboratory assays.
Ausamics TMPPD is supplied as a stable, research-grade powder with consistent performance and batch reproducibility.
Functional Principle
Mode of Action
TMPPD acts as an artificial electron donor in redox reactions.
In the presence of cytochrome c oxidase or related enzymes, TMPPD is oxidised to form a coloured radical cation (Wurster’s blue), enabling rapid qualitative detection.
Applications
Microbiology
Cytochrome oxidase testing for identification of oxidase-positive bacteria
Routine diagnostic microbiology workflows
Biochemistry
Reducing co-substrate for heme peroxidase assays
Redox reaction studies
Electrophoresis & Enzyme Detection
Detection of peroxidase activity on polyacrylamide gels
Visualisation of enzyme bands in gel-based assays
Key Features
Product Advantages
High purity (≥ 97 %) for reliable analytical performance
Rapid colour development for clear visual interpretation
Highly soluble in aqueous buffers and common laboratory solvents
Supplied as a free-flowing powder for accurate weighing
Consistent quality suitable for routine laboratory use
Solubility & Compatibility
Solvent Compatibility
Soluble in water
Soluble in DMSO
Soluble in phosphate-buffered saline (pH ~7.2)
Storage & Stability
Storage Conditions
Store at 4 °C
Protect from light
Keep container tightly sealed
Store away from strong oxidising agents
Stable under recommended storage conditions.
Safety & Regulatory Information
Intended Use
For laboratory research and diagnostic use only.
Not intended for human or veterinary consumption.
Not a therapeutic or clinical diagnostic product.
Refer to the Safety Data Sheet (SDS) before use.
Quality & Supply Assurance
Ausamics Commitment
Supplied with batch-specific Certificate of Analysis (CoA) on request
Controlled manufacturing and packaging
Reliable batch-to-batch consistency
Technical support available from Ausamics Life Science