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TSN Agar (Tryptone Sulfite Neomycin) | AS-1404 | AuSaMicS
$95.00 AUD
Microbiological Culture Media · Food Safety · Anaerobic Bacteriology
TSN Agar (Tryptone Sulfite Neomycin)
Selective isolation and enumeration of Clostridium perfringens
Selective Medium
H₂S Indicator
Standardized Formula
Batch Traceable
AuSaMicS
Laboratory Division
Cat No.
AS-1404
Final pH
7.2 ± 0.2
Dosage
41.07 g/L
Storage
15–30°C
Incubation
37°C Anaerobic
Description
Tryptone Sulfite Neomycin (TSN) Agar is a specialized selective and differential medium engineered for the primary isolation and enumeration of Clostridium perfringens. This refined formulation incorporates dual-antibiotic selectivity and an integrated iron indicator system, making it a critical tool for food safety, dairy microbiology, and water quality laboratories.
Target Mechanism
H₂S Differentiation
Sodium Sulfite and Ammonium Ferric Citrate react to form black precipitates around sulfite-reducing colonies, allowing for visual confirmation of Clostridia.
Dual Selectivity
A precise blend of Neomycin and Polymyxin B suppresses a wide spectrum of competing Gram-positive and Gram-negative flora.
Nutrient Base
Tryptone and Yeast Extract provide high concentrations of essential amino acids and B-complex vitamins to support rapid anaerobic development.
Applications
● Monitoring of Clostridium perfringens in food, dairy, and environmental matrices.
● Detection of sulfite-reducing anaerobes in water treatment microbiology.
● Regulatory microbiological safety assessments in industrial quality control.
Preparation Procedure
| Reconstitution | Suspend 41.07 g of medium in 1 L of purified water. |
| Dissolution | Heat with gentle agitation until completely dissolved. |
| Sterilization | Autoclave at 121°C for 15 minutes. |
| Pouring | Cool to 45–50°C before dispensing into sterile Petri dishes. |
Incubation & Interpretation
Optimal results require anaerobic incubation at 37°C for 18–24 hours. Some strains may require up to 48 hours for complete H₂S reaction.
| Organism | Growth | Colony Appearance |
|---|---|---|
| C. perfringens | Good | Black colonies (H₂S Positive) |
| Clostridium spp. | Variable | Black or grey colonies |
| E. coli / Enterobacter | Inhibited | No growth (Selective Inhibition) |
Standardized Formula (per Liter)
| Tryptone | 15.0 g |
| Yeast Extract | 10.0 g |
| Sodium Sulfite | 1.0 g |
| Ammonium Ferric Citrate | 0.5 g |
| Neomycin Sulfate | 0.05 g |
| Polymyxin B Sulfate | 0.02 g |
| Agar | 15.0 g |
Physicochemical Properties
● Final pH: 7.2 ± 0.2 at 25°C
● Appearance: Dehydrated powder is free-flowing and homogeneous.
Transparency and compliance documentation for AS-1404 is available upon request.
TDS
Technical Data Sheet with complete performance specifications.
SDS
Safety Data Sheet according to GHS standards.
COA
Batch-specific Certificate of Analysis.
AuSaMicS Laboratory Division media are intended for professional laboratory use only. Not for human or animal consumption. Prepared plates should be stored at 2–8°C, protected from light, and used within the specified shelf-life.
Ausamics · Diagnostic Microbiology Media · Cat No: AS-1404