Skip to product information
Request a Quote
Tryptose Broth | AS-1369 | Enriched Medium for Fastidious Bacteria | AuSaMicS
$37.00 AUD
AuSaMicS Life Science • General Enrichment Broth for Fastidious Microorganisms
Tryptose Broth
Tryptose Broth is a nutritive broth used for enrichment and cultivation of streptococci, pneumococci, meningococci, Listeria, pasteurellae, and other pathogenic or fastidious microorganisms. It also serves as a base for preparation of related agar or blood agar systems in classical microbiology workflows.
Cat No.: AS-1369
General Enrichment BrothFastidious SupportAustralian Stock
Format: Dehydrated culture medium SKU: To be assigned Support: COA / technical support available
Application Summary
Professional laboratory use
Reliable workflow integration
Australian stock
Premium AuSaMicS product-page format
Overview & Applications
Tryptose Broth is a nutritive broth used for enrichment and cultivation of streptococci, pneumococci, meningococci, Listeria, pasteurellae, and other pathogenic or fastidious microorganisms. It also serves as a base for preparation of related agar or blood agar systems in classical microbiology workflows.
Primary Applications
- Enrichment and cultivation of fastidious bacteria
- General broth culture in diagnostic and research laboratories
- Base for related agar and blood agar preparations
- Pre-enrichment when low numbers of organisms are expected
Key Features
- Rich tryptose-based nutrient system
- Supports fastidious and pathogenic microorganisms
- Can be adapted into related agar formats
- Useful in pre-enrichment and routine cultivation
Typical Composition / Product Characteristics
| Component / Attribute | Amount / Value | Function | Practical Role |
|---|---|---|---|
| Tryptose | 20.0 g/L | Primary nutrient source | Provides peptides and amino acids |
| D(+) Glucose | 1.0 g/L | Energy source | Supports growth and recovery |
| Sodium chloride | 5.0 g/L | Osmotic balance | Maintains suitable ionic conditions |
| Thiaminium dichloride | 0.005 g/L | Vitamin support | Adds growth factor support |
| Final pH | 7.3 ± 0.2 | Balanced environment | Supports intended cultivation performance |
Preparation, Incubation & Interpretation
Preparation
Suspend 26 g in 1 L purified water and autoclave for 15 minutes at 121°C. Dispense into tubes or flasks as required by the workflow.
Incubation / Use
Incubation depends on the target organism. Many routine applications are performed around 35°C; more demanding organisms may require CO₂-enriched conditions.
Interpretation
Tryptose Broth is a general enrichment and cultivation broth. Interpretation is based on turbidity, downstream subculture, and the target organism protocol rather than a built-in differential reaction.
Comparative Media / Workflow Position
| Medium / Product | Primary Target | Principle | Typical Use | Key Advantage |
|---|---|---|---|---|
| Tryptose Broth | Fastidious/pathogenic bacteria | Rich enrichment broth | General cultivation and pre-enrichment | Broad classical utility |
| Nutrient Broth | Non-fastidious bacteria | Simple nutrient broth | Routine cultivation | Lower complexity |
| Brain Heart Infusion Broth | Very fastidious organisms | Highly enriched broth | Clinical and research use | Very rich nutrient profile |
| Todd Hewitt Broth | Streptococci | Targeted enrichment | Specific workflows | More group-oriented use |
Recommended Workflow
1. Prepare broth
Reconstitute, sterilize, and dispense as needed.
Reconstitute, sterilize, and dispense as needed.
2. Inoculate
Add sample or colony according to the organism workflow.
Add sample or colony according to the organism workflow.
3. Incubate
Use standard or CO₂-enriched incubation conditions.
Use standard or CO₂-enriched incubation conditions.
4. Subculture or assess growth
Use turbidity and downstream plating or identification tests.
Use turbidity and downstream plating or identification tests.
Strategic Use
Advantages
- Rich general broth for fastidious microorganisms
- Useful in classical diagnostic microbiology
- Can serve as a foundation for related agar preparations
Considerations
- Not selective or differential
- Target-specific supplements may still be required for demanding organisms
- Interpretation depends on the downstream workflow
Selection Guide
- Use when a richer classical broth is needed
- Suitable for general fastidious cultivation and enrichment
- Good choice for classical microbiology and teaching workflows