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Urea Broth (Stuart) | AS-1374 | AuSaMicS Melbourne
$29.00 AUD
AuSaMicS Life Science • Differential Media
Urea Broth (Stuart)
High-Buffer Differential Medium
Premium high-buffer differential medium for specific identification of rapid urease producers. Stuart's formulation prevents false positives from weak urease activity through precision pH buffering.
AS-1374 🧪 Differential ✓ Australian Stock 🔬 High Buffer
Same-Day Dispatch: Australian Stock Analytical Grade: Diagnostic applications Filter Sterilized: Heat-sensitive formulation
Differential Media Specialist
High Specificity - Proteus selective
Stuart Formula - Buffer optimized
Complete Package - COA + Protocols
Fast Supply - Ships within 24h
Expert Support - Method optimization
Buffered System
Prevents false positives
Complete Formulation & Buffer System
📋 Stuart's Formulation (per Liter)
Stuart's High-Buffer System Advantage
Buffer Resistance
18.6 g total phosphates resist weak urease pH shifts
18.6 g total phosphates resist weak urease pH shifts
High Specificity
Only rapid, strong urease triggers color change
Only rapid, strong urease triggers color change
Minimal Nutrition
Low yeast extract prevents growth interference
Low yeast extract prevents growth interference
Stuart's Logic:
High Buffer Capacity (18.6 g phosphates) + Minimal Nutrition
↓
Rapid Urease Only = No False Positives
High Buffer Capacity (18.6 g phosphates) + Minimal Nutrition
↓
Rapid Urease Only = No False Positives
Key vs. Christensen: Stuart's 9x higher buffer capacity (18.6g vs 2.0g phosphates) eliminates weak urease false positives from Enterobacteriaceae
Complete Urease Testing Portfolio Analysis
⚖️ Strategic Medium Selection for Urease Testing
✅ Stuart's Broth Advantages
- ✓ Superior Specificity: Buffer system filters weak urease
- ✓ Proteus Specialist: Ideal for UTI pathogen ID
- ✓ Clear Results: Distinct pink color change
- ✓ Standardized Method: USP/EP compliance
- ✓ Liquid Format: Enhanced reactivity
- ✓ Quality Control: Reproducible results
⚠️ Method Considerations
- ▲ Weak Urease Miss: May give false negatives for slow producers
- ▲ Filter Sterilization: Cannot autoclave due to urea
- ▲ Heavy Inoculum: Requires concentrated cell mass
- ▲ Storage Sensitivity: Prepared medium stability
- ▲ Cost Factor: Higher than unbuffered media
🎯 Method Selection Guide
- ◆ Proteus Confirmation: Stuart's Broth (definitive)
- ◆ Comprehensive Screen: Christensen Agar (all urease)
- ◆ Emergency UTI: Rapid Urea Broth (2-4h)
- ◆ H. pylori Testing: CLO Test (biopsy)
- ◆ Point-of-Care: Dipstick methods
📊 Application Performance Matrix
🔬 Stuart's Method Quality Control
Positive Control:
Proteus mirabilis ATCC 25933
Cerise-pink in 2-4 hours
Proteus mirabilis ATCC 25933
Cerise-pink in 2-4 hours
Negative Control:
Escherichia coli ATCC 25922
Remains orange-yellow
Escherichia coli ATCC 25922
Remains orange-yellow
Buffer Verification:
pH 6.8 ± 0.2 maintained
No weak urease false positives
pH 6.8 ± 0.2 maintained
No weak urease false positives
Specificity Test:
Klebsiella pneumoniae
Should remain negative/faint
Klebsiella pneumoniae
Should remain negative/faint
Critical Success Factor: Stuart's buffer system prevents false positives from weak urease producers. Only rapid, strong urease activity (Proteus-type) overcomes the buffer capacity for positive results.
📋 Technical Specifications
| Catalogue Number | AS-1374 |
| Dehydrated Weight | 38.62 g/L |
| Final pH | 6.8 ± 0.2 (at 25°C) |
| Buffer Capacity | High (18.6g phosphates) |
| Sterilization | Filter only (0.2μm) |
| Format | Dehydrated powder |
| Grade | Analytical Grade |
| Shelf Life | 3 years |
Target Activity Profile
- ✓ Proteus mirabilis - Strong positive (cerise)
- ✓ Proteus vulgaris - Strong positive (cerise)
- ✓ Morganella morganii - Strong positive
- ❌ Escherichia coli - Negative (orange)
- ▲ Klebsiella spp. - Weak/negative (filtered)
🧪 Key Applications
- ✓ UTI Pathogen ID: Proteus confirmation
- ✓ Differential Diagnosis: Enterobacteriaceae
- ✓ Quality Control: Urease testing validation
- ✓ Research Applications: Liquid format studies
- ✓ Clinical Laboratories: Confirmatory testing
⚗️ Filter Sterilization Protocol
- 1 Dissolve 38.62g in 1L cold water
- 2 Mix completely (avoid heating)
- 3 Filter sterilize through 0.2μm
- 4 Aseptically dispense into tubes
📦 Storage & Handling
- ✓ Dehydrated: 15-30°C, dry conditions
- ✓ Prepared medium: 2-8°C, 1 week max
- ✓ Light sensitive: Protect phenol red
- ⚠️ No autoclaving: Urea degradation
📄 Documentation
🧪 Complete Urease Testing System
Urease Testing Media Portfolio
Supporting Laboratory Products
Biochemical Testing Systems
Need Differential Media Support?
Our differential media specialists can help optimize your urease testing protocols and buffer systems
For laboratory and research use only. Not intended for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • Analytical grade • Stuart's protocols included