Wort Agar – Microbiological Culture Medium

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Wort Agar – Microbiological Culture Medium

AuSaMicS Life Science™ | www.ausamics.com.au

Product Description
Cat. No. AS-1402
For Laboratory Use Only
Catalog Number
AS-1402
Medium Type
Fungal Culture Medium
Target Organisms
Yeasts & Molds
Final pH
5.4–5.6 ± 0.2
Description

Wort Agar is a nutrient-rich microbiological medium formulated for the isolation, cultivation, and enumeration of yeasts and molds, particularly organisms associated with brewing, fermentation, and beverage microbiology. Malted wort contributes essential carbohydrates, vitamins, and minerals, while peptones provide nitrogen and amino acids to support robust fungal growth.

This medium is especially useful in fermentation research, beverage production quality control, and environmental monitoring programs where recovery of yeasts and molds is required. Its balanced nutrient profile makes it suitable for general fungal cultivation as well as growth of more demanding spoilage organisms encountered in brewery, winery, and food industry settings.

Key Features & Benefits
🍺
Fermentation-Focused Medium Optimized for yeasts and molds associated with brewing, fermentation, and beverage microbiology.
🌾
Nutrient-Rich Formula Malted wort and peptones provide carbohydrates, vitamins, minerals, nitrogen, and amino acids for reliable fungal growth.
🔬
General-Purpose Fungal Medium Suitable for a broad range of yeast and mold species encountered in industrial and research microbiology.
📏
Standardized Performance Designed for reproducible results in quality control, environmental monitoring, and laboratory research.
Applications
🧫
Isolation & Cultivation
  • Isolation and propagation of yeasts and molds from brewing, fermentation, and beverage samples
  • General cultivation of fungi in laboratory settings
🏭
Quality Control
  • Microbiological quality control in breweries, wineries, and food industries
  • Monitoring spoilage organisms in production systems
📈
Research & Monitoring
  • Yeast fermentation and biomass production studies
  • Environmental monitoring in beverage production facilities
Composition & Properties
Malt Extract / Wort20–30 g/L Peptone / Proteose Peptone5 g/L Agar15 g/L (for solid medium) Purified Waterq.s. to 1 L Final pH5.4–5.6 ± 0.2 at 25 °C Recommended Reconstitution40–50 g per 1 L purified water Medium FunctionIsolation, cultivation, and enumeration of yeasts and molds
Preparation
Step Procedure
1 Dissolve 40–50 g of medium in 1 L of purified water.
2 Heat with gentle agitation until fully dissolved.
3 Dispense into Petri dishes, slants, or tubes as required.
4 Sterilize by autoclaving at 121 °C for 15 minutes.
5 Cool to 45–50 °C before pouring plates or preparing slants.
Incubation & Interpretation
Temperature25–30 °C for molds; 25–35 °C for yeasts Duration3–7 days (longer for slow-growing fungi) InterpretationVisible colonies indicate yeast or mold growth. Colony morphology, pigmentation, and sporulation characteristics may assist in species differentiation.
Storage
Powdered Medium15–30 °C, dry and tightly sealed Prepared Plates / Slants2–8 °C, protected from light Shelf LifeUse within the recommended period for optimal performance
⚠ Warning For laboratory use only. Not intended for human, animal, or food consumption.
Disclaimer This product is supplied for laboratory and industrial microbiology use only. Performance depends on correct preparation, sterilization, storage, and incubation conditions. End users are responsible for validating suitability for their intended application and quality system.

Safety Data Sheet

Wort Agar – Microbiological Culture Medium | Cat. AS-1402

SDS — GHS Format
SDS-AS-1402 | Rev. 1.0
Issue Date: March 2026
Section 1 — Identification
Product NameWort Agar – Microbiological Culture Medium Catalog No.AS-1402 Recommended UseIsolation, cultivation, and enumeration of yeasts and molds in laboratory and industrial microbiology SupplierAuSaMicS Pty Ltd, 31 Longview CT, Thomastown VIC 3074, Australia Emergency Contact+61 412 520 598 | support@ausamics.com.au Emergency ServicesAustralia: 000 | Poisons Information Centre: 13 11 26
Section 2 — Hazard Identification
GHS Classification: Not generally classified as hazardous under normal laboratory use. Powder may cause mild irritation to eyes, skin, or respiratory tract if excessive dust is generated.
Signal WordNone required Hazard StatementsNot classified as hazardous. Avoid unnecessary dust formation and contact with eyes. Precautionary StatementsUse good laboratory practice. Avoid inhalation of dust. Wear suitable gloves and eye protection when handling bulk powder. Regulatory StatusFor laboratory use only. Not intended for human, animal, or food consumption.
Section 3 — Composition / Ingredients
Component Typical Content Function
Malt Extract / Wort 20–30 g/L Carbohydrate, vitamin, and mineral source
Peptone / Proteose Peptone 5 g/L Nitrogen and amino acid source
Agar 15 g/L Solidifying agent
Purified Water q.s. to 1 L Solvent / medium base
Section 4 — First Aid Measures
Skin ContactWash with soap and water. Seek medical advice if irritation develops. Eye ContactRinse cautiously with water for several minutes. Seek medical advice if irritation persists. InhalationMove to fresh air if dust is inhaled. Seek medical advice if symptoms persist. IngestionRinse mouth with water. Seek medical advice if large quantities are ingested or discomfort occurs.
Section 5 — Fire-Fighting Measures
Suitable Extinguishing MediaWater spray, foam, dry powder, or carbon dioxide Specific HazardsCombustible organic components may generate smoke and carbon oxides when heated strongly Protective EquipmentUse standard fire-fighting PPE and respiratory protection where needed
Section 6 — Accidental Release Measures
Personal PrecautionsAvoid generating dust. Wear gloves and eye protection during cleanup. Environmental PrecautionsPrevent bulk release to drains and waterways. Clean-Up MethodsSweep or vacuum into suitable container. Wipe remaining residue with damp cloth.
Sections 7–16 — Handling, Storage, PPE, Physical, Stability, Disposal, Transport, Regulatory
HandlingUse good laboratory practices. Avoid inhalation of dust and contact with eyes. StoragePowdered medium: 15–30 °C, dry and tightly sealed. Prepared media: 2–8 °C, protected from light. PPEGloves, safety glasses, and laboratory coat recommended for bulk handling. AppearanceDehydrated powder for microbiological medium preparation Final pH5.4–5.6 ± 0.2 at 25 °C StabilityStable under recommended storage conditions DisposalDispose according to local laboratory and environmental regulations TransportTypically not classified as dangerous goods Prepared ByAuSaMicS Pty Ltd — Quality & Regulatory Affairs
Disclaimer This SDS-style content is provided in website format and should be aligned with your approved internal or regulatory safety documentation before publication where required.

Certificate of Analysis

Wort Agar – Microbiological Culture Medium | Cat. AS-1402

COA — Batch Specific
COA-AS-1402-L001
Issue Date: March 2026
Product Information
Product NameWort Agar – Microbiological Culture Medium Catalog No.AS-1402 Medium TypeFungal culture medium Target OrganismsYeasts and molds Final pH5.4–5.6 ± 0.2 at 25 °C ApplicationIsolation, cultivation, and enumeration of fungi in brewing, beverage, and food microbiology
Lot / Batch Information
Lot NumberAS1402-L001 Manufacture DateMarch 2026 Expiry DateMarch 2029 Storage15–30 °C, dry and tightly sealed AppearanceConforms ✓ Pack SizeTBD
Analytical Test Results
No. Test / Criteria Method Specification / Limit Result Status
1 Appearance Visual inspection Homogeneous dehydrated powder Conforms PASS
2 Colour of Medium Visual inspection Conforms to standard Conforms PASS
3 pH (prepared medium) Potentiometry 5.4–5.6 ± 0.2 at 25 °C 5.5 PASS
4 Gel Strength / Solidification Preparation test Firm gel after sterilization Conforms PASS
5 Growth Promotion Biological performance test Supports growth of representative yeasts and molds Conforms PASS
6 Sterility Check Incubation / QC No contamination in uninoculated control Conforms PASS
Overall Batch Disposition
RELEASED — COMPLIES WITH SPECIFICATIONS
Authorisation
Analysed By
QC Analyst | AuSaMicS Pty Ltd
Date: _______________
Approved & Released By
Quality Manager | AuSaMicS Pty Ltd
Date: _______________
Disclaimer This Certificate of Analysis applies only to the batch identified above and reflects testing of a representative sample. End users should verify suitability under their own operating conditions and quality systems.

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