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X-Gal (BCIG)(CAS: 7240-90-6) | β-Galactosidase Substrate for Blue-White Screening
$25.00 AUD
Documentation
X-Gal (BCIG)
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
Premium chromogenic substrate for blue-white screening and β-galactosidase detection. Essential for molecular cloning, gene expression studies, and microbiological applications.
CAS: 7240-90-6 ASE-906 ✓ Australian Stock
Fast Delivery: 1-3 Business Days Full Documentation: COA + SDS Included
🔬 Mechanism of Action
X-Gal serves as a colorless substrate that undergoes enzymatic cleavage by β-galactosidase (LacZ):
X-Gal + H₂O → Galactose + 5-bromo-4-chloro-3-indoxyl
↓ (spontaneous dimerization)
Blue Indigo Precipitate
↓ (spontaneous dimerization)
Blue Indigo Precipitate
🧬 Key Applications
🔵 Blue-White Screening
Standard method for identifying recombinant E. coli colonies. Blue = empty vector, White = successful insertion.
📊 Reporter Gene Assays
Histochemical detection of lacZ expression in tissues, cells, and developmental studies.
Additional Applications:
- Food Safety: Coliform detection in environmental samples
- ELISA Systems: Enzyme-linked immunosorbent assays
- Industrial QC: Fermentation monitoring and process control
- Research: Promoter studies and gene regulation analysis
💡 Protocol Optimization Tip
Use X-Gal at 40 μg/mL with 0.5mM IPTG for optimal blue-white contrast. Incubate plates at 37°C for 2-4 hours for color development.
📋 Technical Specifications
| Chemical Name | 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside |
| Formula | C₁₄H₁₅BrClNO₆ |
| Molecular Weight | 408.63 g/mol |
| Appearance | White to off-white powder |
| Solubility | DMF (20 mg/mL), DMSO |
| Storage | -20°C, protect from light |
| Working Concentration | 20-40 μg/mL |
| HS Code | 2932.99.00 |
✅ Quality Assurance
- ✓ ≥99% Purity (HPLC verified)
- ✓ Identity Confirmed by ¹H-NMR
- ✓ DNase/RNase Free
- ✓ Functional Activity tested
- ✓ Heavy Metals <10 ppm
🧬 Complete Your Blue-White Screening Kit
IPTG
Isopropyl β-D-thiogalactopyranoside
LB Agar
Ampicillin
Sodium salt, sterile
Need Technical Support?
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For laboratory research use only. Not for human or therapeutic use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • Full documentation included