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X-Glc (X-glucoside) | ASE-902 | Chromogenic beta-D-Glucosidase Substrate
$55.00 AUD
Enzyme Substrates · Microbiology · Diagnostic Reagents
X-Glc (X-Glucoside)
5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside · CAS 15548-60-4
chromogenic, ≥97%, powder
β-D-Glucosidase Substrate Chromogenic Indicator Molecular Grade High Purity
AuSaMicS
Laboratory Division
Cat No.
ASE-902
CAS No.
15548-60-4
Target
β-Glucosidase
HS Code
2934.99.90
Storage
-20°C, Dry
Description
X-Glc (5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside) is a high-purity indoxyl glycoside developed for the visual detection of β-D-glucosidase activity. As a chromogenic substrate, it is essential in diagnostic microbiology for the differentiation of microorganisms—particularly Listeria spp. and Enterococci—on solid-phase systems such as agar plates and membrane filters.
Mechanism of Action
Upon enzymatic hydrolysis by β-glucosidase, the glucose moiety is cleaved to release an indoxyl intermediate. This intermediate undergoes rapid oxidative dimerization to form an insoluble, intense blue indigo precipitate (5,5'-dibromo-4,4'-dichloro-indigo), providing sharp, localized color development at the site of enzyme activity.
Product Synonyms
● X-Glucoside
● 5-Bromo-4-chloro-3-indolyl-beta-D-glucoside
Key Applications
Listeria Differentiation
Used in specialized agars (e.g., ALOA) to differentiate Listeria monocytogenes based on β-glucosidase expression.
Enterococci Testing
Essential for the identification of Enterococcus spp. in water and food microbiology workflows.
Plant Pathology
Used in plant biology to monitor the expression of the gusA or similar reporter genes in transgenic tissues.
Chemical Profile
| Catalog Number | ASE-902 |
| CAS Number | 15548-60-4 |
| Molecular Formula | C₁₄H₁₅BrClNO₆ |
| Molecular Weight | 408.6 g/mol |
| Appearance | White to off-white crystalline powder |
Analytical Specifications
| Purity (HPLC) | ≥98.0% |
| Solubility | Soluble in DMF or DMSO |
| Identity (NMR) | Conforms to structure |
Storage & Handling
● Storage: Store at −20°C in a desiccated environment.
● Light Sensitivity: Protect from light to ensure substrate stability.
● Handling: Wear standard laboratory PPE; handle under low-light conditions if possible.
AuSaMicS Laboratory Division provides full traceability and quality assurance for all diagnostic substrates. Documentation is available with every batch.
TDS
Technical Data Sheet with preparation and solvent guidelines.
SDS
Safety Data Sheet according to GHS standards.
COA
Batch-specific Certificate of Analysis verifying purity and NMR identity.
Important Notice: For laboratory research and industrial use only. Not for human, animal, or therapeutic use. Handle in accordance with Good Laboratory Practice (GLP).
Ausamics · Diagnostic Reagents Division · Cat No: ASE-902