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Yeast Glucose Chloramphenicol Agar (YGC), Selective Medium for Yeast & Mold Isolation, AS-1381
$29.00 AUD
AuSaMicS Life Science · Food & Environmental Mycology
Yeast Glucose Chloramphenicol Agar (YGC)
Selective Medium for Yeast & Mold Isolation | Catalogue No. AS-1381
Chloramphenicol-selective dehydrated agar for reliable recovery and enumeration of yeasts and molds from food, beverage, pharmaceutical, cosmetic, water, and environmental samples.
AS-1381 pH 6.6 ± 0.2 40 g/L Chloramphenicol Selective ✓ Oxoid CM0920 Equivalent ✓ BD Difco 212490 Equivalent ✓ ISO 21527 Compatible 🇦🇺 Melbourne Stock ⚡ Same-Week Dispatch
pH (25°C)
6.6 ± 0.2
Optimal for fungi
Dissolution
40 g/L
In distilled water
Incubation
25°C
3–7 days
Selective Agent
0.1 g/L
Chloramphenicol
📄 Full DocumentationCOA · TDS · SDS every batch
🇦🇺 Australian StockNo import lead times
⚡ Same-Week DispatchMelbourne warehouse
🔬 Technical SupportFrom our science team
Overview
Yeast Glucose Chloramphenicol Agar (YGC Agar, AS-1381) is a dehydrated selective culture medium for the isolation, cultivation, and enumeration of yeasts and molds from food, beverage, pharmaceutical, cosmetic, water, and environmental samples. Chloramphenicol (0.1 g/L) inhibits Gram-positive and Gram-negative bacteria, while the elevated glucose concentration (20 g/L) and slightly acidic pH (6.6) support robust recovery of a broad range of yeasts and filamentous molds.
YGC Agar is compatible with ISO 21527 workflows for yeast and mold enumeration in food and animal feeding stuffs, and is widely used in pharmaceutical microbial limit testing, cosmetic QC, and environmental monitoring. AuSaMicS AS-1381 is equivalent to Oxoid CM0920 and BD Difco 212490, manufactured and packed locally in Melbourne with same-week dispatch.
Why Chloramphenicol? — Principle of Selectivity
Chloramphenicol (0.1 g/L) is a broad-spectrum bacteriostatic antibiotic that inhibits bacterial protein synthesis by binding to the 50S ribosomal subunit. At 0.1 g/L it effectively suppresses both Gram-positive and Gram-negative bacteria without significant inhibitory effect on fungi, which use 80S ribosomes insensitive to chloramphenicol.
Why not acidification? Earlier fungal selective media relied on lowering pH to ~3.5 to suppress bacteria. This approach inhibits some acid-sensitive yeast and mold species and introduces a variable that reduces recovery rates. Chloramphenicol selectivity at near-neutral pH (6.6) gives consistently higher and more accurate fungal recovery across a wider range of species.
Why not Rose Bengal? Rose bengal restricts colony spread — useful in heavily contaminated samples — but can inhibit some fungal species and interferes with morphology assessment. YGC Agar is preferred when accurate colony counts and species identification are the priority.
Organisms — Growth Profile
| Organism | Growth | Application |
|---|---|---|
| Candida albicans ATCC 10231 | ✓ Good to excellent | Food, pharmaceutical, environmental QC |
| Saccharomyces cerevisiae ATCC 9763 | ✓ Excellent growth | Beverage microbiology, food testing |
| Aspergillus brasiliensis ATCC 16404 | ✓ Good to excellent | Pharmaceutical microbial limit testing |
| Penicillium spp. | ✓ Good growth | Food spoilage investigation |
| Fusarium spp. | ✓ Good growth | Environmental and grain mycology |
| Rhizopus spp. | ✓ Moderate to good | Food spoilage, environmental monitoring |
| Escherichia coli ATCC 25922 | ✗ Inhibited | Selectivity indicator |
| Staphylococcus aureus ATCC 25923 | ✗ Inhibited | Selectivity indicator |
| Bacillus subtilis ATCC 6633 | ✗ Inhibited | Selectivity indicator |
Applications
🥫
Food & Beverage QC
Yeast and mold counts in dairy, bakery, beverages, sauces
💊
Pharmaceutical QC
Microbial limit testing per USP, EP, TGA guidelines
💄
Cosmetics QC
Microbial limit and challenge testing per ISO 17516
💧
Water Microbiology
Fungal monitoring in treated water and process water
🌬️
Environmental Monitoring
Air settle plates, surface swabs, cleanroom monitoring
🔬
Research & Teaching
Fungal physiology, mycology courses, QC method development
YGC vs Other Fungal Selective Media
| Medium | Selective Agent | Best For | Limitation |
|---|---|---|---|
| YGC Agar (AS-1381) ★ | Chloramphenicol 0.1 g/L | Broad yeast & mold recovery; pharma, food, environmental QC; species ID | Colonies may spread — limit incubation to 5 days for dense samples |
| Rose Bengal Chloramphenicol Agar | Chloramphenicol + Rose Bengal dye | Heavily contaminated samples; colony spread restriction | Rose bengal may inhibit some species; interferes with morphology |
| Dichloran Rose Bengal Agar (DRBC) | Dichloran + Rose Bengal | ISO 21527-2 food testing; restricted colony spread | Some mold inhibition; not suitable for all pharma applications |
| Sabouraud Dextrose Agar (SDA) | None / low pH optional | General fungal isolation; clinical dermatophytes | Not selective for bacteria — cannot be used in non-sterile samples without supplement |
| Potato Dextrose Agar (PDA) | None / pH adjustment | General mold sporulation; food testing | Bacterial overgrowth in mixed samples |
Storage & Shelf Life
Powder: Store in a cool, dry location, tightly sealed, protected from moisture and direct light. Protect chloramphenicol from prolonged UV exposure.
Prepared plates: Store at 2–8°C in sealed bags, protected from light. Use within 2 weeks of preparation.
Shelf life: Stated on label — typically 3–5 years from manufacture under recommended conditions.
Prepared plates: Store at 2–8°C in sealed bags, protected from light. Use within 2 weeks of preparation.
Shelf life: Stated on label — typically 3–5 years from manufacture under recommended conditions.
Cross-Reference / Equivalents
| Brand | Product Name | Cat. No. | Notes |
|---|---|---|---|
| Oxoid (Thermo Fisher) | Yeast Glucose Chloramphenicol Agar | CM0920 | Imported |
| BD Difco | YM Agar / Chloramphenicol Glucose Agar | 212490 | Imported |
| HiMedia | Yeast Glucose Chloramphenicol Agar | M403 | Imported |
| Merck (Sigma-Aldrich) | YGC Agar | 1.16000 | Imported |
| AuSaMicS | YGC Agar | AS-1381 | Melbourne stock · COA · same-week dispatch |
Intent-Based Search Queries
YGC agar Australia yeast mold isolation medium Melbourne Oxoid CM0920 alternative Australia ISO 21527 yeast mold medium pharmaceutical microbial limit testing agar chloramphenicol selective agar fungi food microbiology yeast mold count agar cosmetic QC fungal medium Australia
Related Products
Product Identification
| Product Name | Yeast Glucose Chloramphenicol Agar (YGC Agar) |
| Catalogue Number | AS-1381 |
| Synonyms | YGC Agar; Chloramphenicol Glucose Yeast Extract Agar; Yeast Mold Selective Agar |
| Medium Type | Selective solid culture medium (agar) |
| Form | Dehydrated powder; prepared medium: pale amber agar |
| Equivalents | Oxoid CM0920 | BD Difco 212490 | HiMedia M403 | Merck 1.16000 |
| Standard | ISO 21527 compatible |
| HS / AHECC Code | 3821.00.00 |
Composition (per litre of prepared medium)
| Ingredient | g/L | CAS | Function |
|---|---|---|---|
| Glucose (D-Glucose) | 20.0 | 50-99-7 | Primary carbon and energy source; elevated concentration supports rapid, robust fungal growth |
| Yeast Extract | 5.0 | 8013-01-2 | B-complex vitamins, amino acids, nucleotides, and accessory growth factors essential for fungal metabolic activity |
| Chloramphenicol | 0.1 | 56-75-7 | Broad-spectrum bacteriostatic antibiotic — inhibits bacterial 50S ribosomal protein synthesis; selective inhibition of Gram-positive and Gram-negative bacteria without effect on fungi |
| Agar | 14.9 | 9002-18-0 | Solidifying agent; gel at standard concentration supports colony development and morphology assessment |
Total: 40.0 g/L | Final pH 6.6 ± 0.2 at 25°C | Autoclave 121°C / 15 min
Physicochemical Specifications
| Parameter | Specification | Method |
|---|---|---|
| Appearance (powder) | Beige to light cream, free-flowing powder | Visual |
| Appearance (prepared medium) | Pale amber, clear agar | Visual after dissolution |
| pH (prepared, 25°C) | 6.6 ± 0.2 | Potentiometric (calibrated electrode) |
| Moisture Content | ≤ 5.0% | Loss on drying, 105°C |
| Dissolution | Complete at 40 g/L with heating | Visual inspection |
| Storage (powder) | Cool, dry, tightly sealed, protected from moisture and light | — |
| Storage (prepared plates) | 2–8°C, sealed bags, protected from light. Use within 2 weeks. | — |
Preparation Protocol
1
Suspend 40 g of dehydrated YGC Agar (AS-1381) in 1 litre of distilled or deionised water. Mix to disperse the powder.
2
Heat with continuous agitation until completely dissolved. The medium should appear pale amber and clear. Bring just to boiling.
3
Autoclave at 121°C for 15 minutes. Do not overheat — excessive heat degrades chloramphenicol and darkens the medium.
4
Cool to 45–50°C in a water bath before pouring. Aseptically pour approximately 20 mL per 90 mm Petri dish. Allow to solidify completely.
5
Inoculate and incubate at 25°C for 3–5 days. Examine from day 3. Allow up to 7 days for slow-growing filamentous molds. Do not invert plates — colony spread assessment requires upright incubation.
6
Count and record colonies. For quantitative enumeration, count between 15–150 colonies per plate for statistical accuracy. Report as CFU/g or CFU/mL of original sample.
💡 Tip: For air settle plates and surface monitoring, use standard incubation without inversion. For food and beverage samples with high expected counts, prepare serial dilutions before plating to avoid TNTC (Too Numerous To Count) results.
QC Performance — Expected Results
| Test Organism | ATCC Strain | Expected Result (25°C / 5 days) |
|---|---|---|
| Candida albicans | ATCC 10231 | Good to excellent growth — cream to white yeast colonies |
| Aspergillus brasiliensis | ATCC 16404 | Good to excellent growth — black conidial heads characteristic |
| Saccharomyces cerevisiae | ATCC 9763 | Good growth — white to cream colonies |
| Escherichia coli | ATCC 25922 | Inhibited — no significant growth |
| Staphylococcus aureus | ATCC 25923 | Inhibited — no significant growth |
| Bacillus subtilis | ATCC 6633 | Inhibited — no significant growth |
Mode of Action
YGC Agar operates through two complementary mechanisms:
| Mechanism | Component | Effect |
|---|---|---|
| Antibiotic selectivity | Chloramphenicol 0.1 g/L | Binds 50S bacterial ribosomal subunit; bacteriostatic for Gram-positive and Gram-negative organisms; fungi use 80S ribosomes — unaffected |
| Nutritional enrichment | Glucose 20 g/L + Yeast Extract 5 g/L | High glucose supports rapid fungal carbon metabolism; yeast extract provides vitamins and growth factors for diverse fungal species including slow-growing molds |
| pH optimisation | pH 6.6 ± 0.2 | Near-neutral pH optimal for most fungi; slightly below neutral provides mild additional suppression of some bacteria without restricting acid-sensitive fungal species |
Literature References
1. Mossel DAA, Visser M, Mengerink WHJ. A comparison of media for the enumeration of molds and yeasts in foods and beverages. Laboratory Practice. 1962;11:109–112. [Original YGC medium reference]
2. ISO 21527-1:2008. Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of yeasts and moulds — Part 1: Colony count technique in products with water activity greater than 0.95. ISO, Geneva.
3. ISO 21527-2:2008. Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of yeasts and moulds — Part 2: Colony count technique in products with water activity less than or equal to 0.95. ISO, Geneva.
4. European Pharmacopoeia (EP) 11th Edition. 2.6.12 Microbiological Examination of Non-Sterile Products. Council of Europe, Strasbourg.
5. ISO 11133:2014. Culture media — Preparation, production, storage and performance testing. ISO, Geneva.
Download TDS (PDF)📄 Full 16-section GHS SDS available — Australian WHS Regulations 2023 / GHS 7th Edition. Download below or request via support@ausamics.com.au
Section 1 — Identification
| Product Name | Yeast Glucose Chloramphenicol Agar (YGC Agar) |
| Catalogue No. | AS-1381 |
| Supplier | AuSaMicS Pty Ltd | ABN 56 676 640 467 |
| Address | 31 Longview CT, Thomastown VIC 3074, Australia |
| Phone / Email | +61 412 520 598 | support@ausamics.com.au |
| Emergency | Poisons Information Centre: 13 11 26 (Australia, 24 hr) |
| Intended Use | Selective culture medium for yeast and mold isolation and enumeration. For laboratory use only. |
Section 2 — Hazard Identification
⚠️ Contains Chloramphenicol (CAS 56-75-7) — classified as GHS Hazardous Substance.
Chloramphenicol: Suspected carcinogen (Category 2). May cause blood disorders (aplastic anaemia) with repeated or prolonged exposure. Handle with appropriate PPE. Avoid skin contact, inhalation of powder dust, and eye contact.
Chloramphenicol: Suspected carcinogen (Category 2). May cause blood disorders (aplastic anaemia) with repeated or prolonged exposure. Handle with appropriate PPE. Avoid skin contact, inhalation of powder dust, and eye contact.
| GHS Classification | Carcinogenicity Category 2 (H351 — Suspected of causing cancer) due to chloramphenicol content. The product as formulated (0.1 g/L in dehydrated powder) presents low acute risk at intended use concentrations. |
| Signal Word | WARNING |
| Hazard Statements | H351: Suspected of causing cancer (chloramphenicol component) |
| Precautionary Statements | P201: Obtain special instructions before use. P202: Do not handle until all safety precautions have been read. P280: Wear protective gloves and eye protection. P308+P313: If exposed or concerned, seek medical advice. |
Composition
| Component | CAS | g/L | GHS Hazard |
|---|---|---|---|
| Glucose | 50-99-7 | 20.0 | Not classified |
| Yeast Extract | 8013-01-2 | 5.0 | Not classified |
| Chloramphenicol | 56-75-7 | 0.1 | H351 Suspected carcinogen (Cat. 2) |
| Agar | 9002-18-0 | 14.9 | Not classified |
Sections 4–16 Summary
| Section | Summary |
|---|---|
| 4. First Aid | Inhalation: remove to fresh air. Skin: wash with soap/water immediately — remove contaminated clothing. Eye: flush water 15 min — seek medical attention. Ingestion: rinse mouth; seek medical advice if symptomatic. |
| 5. Fire Fighting | Not flammable. CO2, dry chemical, foam, water spray. Combustion: CO2, CO, NOx, HCl (from chloramphenicol). |
| 6. Accidental Release | Wear full PPE. Avoid dust generation. Collect powder carefully in sealed containers. Label as chloramphenicol-containing waste. Do not allow to enter drains or waterways. |
| 7. Handling & Storage | Handle in dedicated area with LEV. Wear nitrile gloves, safety spectacles, lab coat. Store cool, dry, tightly sealed. Protect from UV. Keep from oxidising agents. Prepared plates: 2–8°C, sealed, protected from light. |
| 8. Exposure Controls / PPE | Nitrile gloves (minimum 0.1 mm). Safety spectacles or chemical splash goggles. Lab coat. Local exhaust ventilation (LEV) when handling powder. OEL for chloramphenicol: as low as reasonably practicable. |
| 9. Physical & Chemical | Powder: beige, free-flowing. Prepared: pale amber agar. pH 6.6 ± 0.2. Not flammable, not explosive, not oxidising. |
| 10. Stability | Stable under recommended conditions. Chloramphenicol degrades on prolonged exposure to UV light or strong alkaline conditions. Avoid excess heat during preparation. |
| 11. Toxicology | Chloramphenicol: suspected carcinogen (H351); associated with aplastic anaemia in humans with repeated exposure. Low acute toxicity at formulated concentration but precautionary measures required due to carcinogenicity classification. |
| 12. Ecology | Chloramphenicol is an antimicrobial — avoid discharge to waterways or soil. Environmental contamination may promote antimicrobial resistance in environmental microorganisms. |
| 13. Disposal | Dispose as chemical waste containing a classified hazardous substance (chloramphenicol). Inoculated plates: autoclave before disposal. Consult VIC EPA guidelines for chloramphenicol-containing waste disposal. Do NOT dispose to domestic drain. |
| 14. Transport | Not classified as dangerous goods under ADG, IATA, or IMDG at the formulated concentration. No UN Number. Transport in sealed, labelled containers. |
| 15. Regulatory | Australian WHS Regulations 2023 / GHS 7th Edition. H351 classification due to chloramphenicol. HS 3821.00.00. For laboratory use only. Chloramphenicol is a Schedule 4 substance in some contexts — confirm applicable requirements with your institutional biosafety officer. |
| 16. Other Info | SDS-AS-1381-YGC | Issue: January 2026 | Review: January 2028 | Prepared by Hassan Salimi, AuSaMicS Pty Ltd. |
Certificate of Analysis — AS-1381
Each batch of Yeast Glucose Chloramphenicol Agar (AS-1381) is manufactured under quality-controlled conditions and released with a batch-specific Certificate of Analysis confirming compliance with all physicochemical and microbiological performance specifications per ISO 11133:2014.
Analytical Results — Typical Specifications
| # | Test Parameter | Specification | Method | Result |
|---|---|---|---|---|
| 1 | Appearance (powder) | Beige to light cream, free-flowing | Visual | Conforms |
| 2 | Appearance (prepared medium) | Pale amber, clear agar | Visual | Conforms |
| 3 | pH (prepared, 25°C) | 6.6 ± 0.2 | Potentiometric | Conforms |
| 4 | Moisture Content | ≤ 5.0% | LOD 105°C | Conforms |
| 5 | Candida albicans ATCC 10231 (positive) | Good to excellent growth within 5 days at 25°C | Colony count / visual | Conforms |
| 6 | Aspergillus brasiliensis ATCC 16404 (positive) | Good to excellent growth within 5 days at 25°C | Colony count / visual | Conforms |
| 7 | Saccharomyces cerevisiae ATCC 9763 (positive) | Good growth within 3–5 days at 25°C | Colony count / visual | Conforms |
| 8 | Escherichia coli ATCC 25922 (negative) | Inhibited — no significant growth | Visual | Conforms |
| 9 | Staphylococcus aureus ATCC 25923 (negative) | Inhibited — no significant growth | Visual | Conforms |
| 10 | Bacillus subtilis ATCC 6633 (negative) | Inhibited — no significant growth | Visual | Conforms |
CONCLUSION: BATCH CONFORMS TO ALL STATED SPECIFICATIONS — RELEASED FOR SUPPLY
Batch Traceability
📋 Batch Details — Stated on Label and Supplied COA
Product
Yeast Glucose Chloramphenicol Agar
Catalogue No.
AS-1381
Selective Agent
Chloramphenicol 0.1 g/L
Standard
ISO 21527 compatible
Batch Number
Stated on label
Manufacturing Date
Stated on label
Expiry Date
Stated on label (3–5 years)
Storage
Cool, dry, sealed, protected from light
Manufacturer
AuSaMicS Pty Ltd — Melbourne, Australia
COA Status
Included with every order
Frequently Asked Questions
❓ What organisms does YGC Agar isolate?
YGC Agar recovers a broad range of yeasts and molds including Candida spp., Saccharomyces spp., Aspergillus spp., Penicillium spp., Fusarium spp., and most food, environmental, and pharmaceutical spoilage fungi. Bacteria are suppressed by chloramphenicol (0.1 g/L).
❓ Is AS-1381 equivalent to Oxoid CM0920?
Yes — AS-1381 follows the same Yeast Glucose Chloramphenicol Agar formulation with identical pH (6.6 ± 0.2) and dissolution (40 g/L). Equivalent to Oxoid CM0920, BD Difco 212490, HiMedia M403, and Merck 1.16000. Manufactured locally in Melbourne with same-week dispatch and batch COA.
❓ YGC vs Rose Bengal Chloramphenicol Agar — which do I use?
Use YGC Agar when accurate colony counts and clear morphology are the priority — pharmaceutical QC, cosmetic testing, food testing with low to moderate fungal loads, and species identification. Use Rose Bengal Chloramphenicol Agar for heavily contaminated samples where colony spread would be problematic.
❓ What safety precautions are needed for YGC Agar?
YGC Agar contains chloramphenicol (0.1 g/L), a suspected carcinogen (GHS H351) associated with aplastic anaemia on prolonged exposure. Always wear nitrile gloves and safety spectacles when handling the powder or prepared plates. Use in a well-ventilated area or under local exhaust ventilation. Dispose of as chloramphenicol-containing chemical waste — do not dispose to drain. Autoclave inoculated plates before disposal.
AuSaMicS Pty Ltd — Quality Assurance
✓ ISO 11133:2014 compliant QC — 3 positive + 3 negative control organisms per batch
✓ ISO 21527 compatible formulation
✓ COA, TDS, and full GHS SDS with every order
✓ Australian stock — same-week dispatch, no import lead times
✓ ISO 11133:2014 compliant QC — 3 positive + 3 negative control organisms per batch
✓ ISO 21527 compatible formulation
✓ COA, TDS, and full GHS SDS with every order
✓ Australian stock — same-week dispatch, no import lead times
⚠️ Contains chloramphenicol — a suspected carcinogen (H351). For laboratory use only. Not for food, pharmaceutical ingredient, medical, or veterinary use. AuSaMicS Pty Ltd accepts no liability for use outside the intended application.
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