Yeast Extract Peptone Dextrose (YPD) Agar
Yeast Extract Peptone Dextrose (YPD) Agar
Nutrient-Rich Non-Selective Medium for Yeast Cultivation
Product Identification
Chemical & Physical Information
Product Name: Yeast Extract Peptone Dextrose Agar
Synonyms: YPD Agar; YEPD Agar; Yeast Extract Peptone Dextrose Medium
Catalogue Number: AS-1384
Medium Type: Non-selective solid culture medium
Form: Dehydrated powder
Product Description
Yeast Extract Peptone Dextrose (YPD) Agar is a nutrient-rich, non-selective solid culture medium widely used for the cultivation, propagation, and maintenance of yeasts, particularly Saccharomyces cerevisiae. Its balanced formulation provides readily assimilable nitrogen sources, vitamins, and carbohydrates, supporting rapid and robust yeast growth.
YPD Agar is a standard medium in molecular biology, yeast genetics, fermentation research, and industrial biotechnology, where reliable growth and high biomass yield are required. The non-selective nature of the medium allows growth of a broad range of yeast strains without selective pressure.
Ausamics YPD Agar is supplied as a high-quality dehydrated powder to ensure consistent solidification, clarity, and batch-to-batch reproducibility.
Principle of the Medium
Functional Components
Yeast Extract: Supplies B-complex vitamins, growth factors, and amino acids
Peptone: Provides peptides and nitrogenous compounds
Dextrose (Glucose): Primary fermentable carbohydrate and energy source
Agar: Solidifying agent providing a stable growth surface
Typical Characteristics
Medium Properties
Appearance (dehydrated): Light beige to tan powder
Appearance (prepared): Clear to slightly opalescent amber agar
pH (prepared medium): 6.5 ± 0.2 at 25 °C
Selectivity: Non-selective
Differential: No
Composition
Standard Formula (per litre)
Yeast Extract: 10.0 g
Peptone: 20.0 g
Dextrose (Glucose): 20.0 g
Agar: 15.0 g
Typical Applications
Yeast Cultivation & Maintenance
Routine culturing of Saccharomyces cerevisiae
Propagation and maintenance of laboratory yeast strains
Molecular Biology & Genetics
Yeast transformation and expression studies
Plasmid maintenance and phenotype analysis
Fermentation & Industrial Biotechnology
Yeast growth studies for fermentation optimisation
Bioprocess development and strain screening
Education & Research
Teaching laboratories and microbiology practicals
General yeast physiology and metabolism studies
Preparation Instructions
Medium Preparation
Suspend the required quantity of dehydrated medium in purified water
Heat with agitation until fully dissolved
Sterilise by autoclaving at 121 °C for 15 minutes
Cool to 45–50 °C
Pour into sterile Petri dishes
Storage & Stability
Storage Conditions
Dehydrated medium: Store at 15–30 °C in a cool, dry place
Keep container tightly closed and protected from moisture
Prepared plates: Store at 2–8 °C
Use within the recommended period to ensure optimal performance
Intended Use
Regulatory Statement
For laboratory research, industrial biotechnology, and educational use only.
Not intended for medical, diagnostic, food, or veterinary use.
Quality & Supply Assurance
Manufacturing & Documentation
Manufactured under controlled conditions
Consistent batch-to-batch performance
Certificate of Analysis (COA) available upon request
Technical support provided by Ausamics Life Science
Customs & Trade Information
Classification
HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms