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5-Bromo-6-chloro-3-indolyl phosphate, disodium salt, BCIP Disodium Salt (Magenta-Phos) - ASE-911
  • 5-Bromo-6-chloro-3-indolyl phosphate, disodium salt, BCIP Disodium Salt (Magenta-Phos) - ASE-911
BCIP Disodium Salt (Magenta-Phos)

BCIP Disodium Salt (Magenta-Phos)

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AuSaMicS Life Science • Enzyme Substrates

BCIP (Magenta-Phos)

5-Bromo-6-chloro-3-indolyl phosphate disodium salt

Premium chromogenic substrate for alkaline phosphatase detection. Essential for Western blotting, immunohistochemistry, and ELISA systems with distinctive magenta-purple signal development.

CAS: 404366-59-2 ASE-911 ✓ Australian Stock 🟣 Alkaline Phosphatase
Same-Day Dispatch: Australian Stock Research Grade: Western blot quality Stable: Monohydrate crystal form

Western Blot Specialist

Magenta Signal - High contrast detection
Research Grade - Immunoblotting quality
Complete Package - COA + SDS included
Fast Supply - Ships within 24h
Expert Support - Protocol optimization
NBT-BCIP Ready
Pairs with NBT for dual-color systems

🔬 Alkaline Phosphatase Detection Mechanism

BCIP undergoes alkaline phosphatase-catalyzed dephosphorylation, releasing 5-bromo-6-chloro-3-indoxyl which rapidly oxidizes to form a distinctive colored precipitate:

BCIP + H₂O
↓ (Alkaline phosphatase)
5-Bromo-6-chloro-3-indoxyl + Phosphate
↓ (Spontaneous oxidation & dimerization)
Magenta-Purple Precipitate
Enhanced Detection: Often combined with NBT for enhanced color development and sensitivity

Complete Chromogenic Substrate Library

Substrate Target Enzyme Signal Key Applications Typical Usage
X-Gal β-Galactosidase Blue Molecular cloning, blue-white screening 20-40 μg/mL
X-Gluc β-Glucuronidase Blue E. coli detection, GUS reporter 50-100 μg/mL
X-GlcNAc N-acetyl-β-D-glucosaminidase Blue Yeast identification, fungal diagnostics 50-100 mg/L
BCIP Alkaline phosphatase Magenta Western blot, immunohistochemistry 150-330 μg/mL
Salmon-Gal β-Galactosidase Red Visual contrast, multiplex assays 20-40 μg/mL
Salmon-Caprylate C8 Esterase Red Salmonella detection, food safety 50-100 μg/mL
Magenta-Gal β-Galactosidase Purple Alternative screening, yellow media 20-40 μg/mL
ONPG β-Galactosidase Yellow Quantitative assays, biochemical tests 1-4 mg/mL
MUG β-Glucuronidase Fluorescent Fluorogenic detection, water testing 50-100 μg/mL

🧬 Research & Diagnostic Applications

Western Blotting

Standard substrate for alkaline phosphatase-conjugated antibodies in protein detection protocols.

🔬 Immunohistochemistry

Tissue section staining with magenta signal for high-contrast visualization against tissue backgrounds.

Specialized Research Applications:

  • ELISA Systems: Endpoint detection in immunoassays
  • Dot Blotting: Rapid protein detection protocols
  • In Situ Hybridization: RNA/DNA localization studies
  • Flow Cytometry: Cell surface marker analysis
  • Tissue Arrays: High-throughput screening
  • Diagnostic Kits: Commercial assay development

 NBT-BCIP Dual-Substrate System

The NBT-BCIP system combines two chromogenic substrates for enhanced alkaline phosphatase detection:

NBT (Nitro Blue Tetrazolium)
Produces purple-blue precipitate
BCIP (This Product)
Produces magenta precipitate
Combined Result: Dark purple-black precipitate with enhanced sensitivity and contrast

Protocol Optimization Guidelines

Working Concentration:
150-330 μg/mL (solo use)
NBT-BCIP System:
165 μg/mL BCIP + 330 μg/mL NBT
pH Optimum:
9.0-9.5 (alkaline phosphatase activity)
Storage:
4°C, protect from light & moisture

Technical Specifications

Chemical Name 5-Bromo-6-chloro-3-indolyl phosphate disodium salt
Salt Form Disodium salt monohydrate
CAS Number 404366-59-2
Formula C₈H₄BrClNNa₂O₄P·H₂O
Molecular Weight 388.46 g/mol
Purity ≥98% (HPLC)
Appearance White to pale yellow powder
Solubility Water (1 mg/mL), DMSO, DMF
Storage 2-8°C, protect from light
HS Code 2933.99.00

Alternative Names

BCIP
Magenta-Phos
5-Bromo-6-chloro-3-indoxyl phosphate
Sodium 5-bromo-6-chloro-1H-indol-3-yl phosphate
BCIP disodium salt monohydrate

Buffer Requirements

  • ✓ pH Buffer: Tris-HCl pH 9.0-9.5
  • ✓ Mg²⁺ Required: MgCl₂ (1-5 mM)
  • ✓ NaCl: 100-150 mM ionic strength
  • ✓ Optional NBT: Enhanced signal intensity

✅ Quality Standards

  • ✓ HPLC Purity: ≥98% verified
  • ✓ Enzymatic Activity: Functional validation
  • ✓ Hydrate Content: Karl Fischer verified
  • ✓ Heavy Metals: <10 ppm total
  • ✓ Storage Stability: Validated conditions

🧬 Complete Western Blotting System

Buffers & Detection Reagents

NBT

Nitro Blue Tetrazolium

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Tris-HCl Buffer

pH 9.0 alkaline buffer

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Magnesium Chloride

Enzyme cofactor

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PVDF Membranes

Western blot transfer

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Antibodies & Conjugates

Anti-Rabbit AP

Alkaline phosphatase conjugate

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Anti-Mouse AP

Alkaline phosphatase conjugate

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Anti-Goat AP

Alkaline phosphatase conjugate

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Streptavidin-AP

Biotin detection system

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Blocking & Wash Reagents

BSA

Bovine serum albumin

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Skim Milk Powder

Non-fat dry milk blocking

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Tween-20

Non-ionic detergent

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TBST Buffer

Tris-buffered saline + Tween

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For laboratory, research, and industrial use only. Not for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • Full documentation included

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