Skip to product information
AC Broth (Actinomycete Isolation Broth)
  • AC Broth (Actinomycete Isolation Broth)
  • AC Broth (Actinomycete Isolation Broth)
  • AC Broth (Actinomycete Isolation Broth)
  • AC Broth (Actinomycete Isolation Broth)
AC Broth (Actinomycete Isolation Broth)

AC Broth (Actinomycete Isolation Broth)

$45.00
Weight

AC Broth (Actinomycete Isolation Broth)

Selective Enrichment Broth for Isolation of Actinomycetes

Catalog Number: AS-1103

Overview

AC Broth (Actinomycete Isolation Broth) is a selective liquid culture medium formulated for the enrichment and cultivation of actinomycetes, including Streptomyces, Nocardia, Actinomadura, and other rare actinobacterial genera.

It is the liquid counterpart of Actinomycete Isolation Agar (AC Agar) and is specifically designed for use with heavily contaminated environmental samples such as soil, plant material, sediments, and compost. The formulation suppresses competing Gram-negative bacteria, many Gram-positive bacteria, and fast-growing fungi, allowing slow-growing actinomycetes to proliferate during extended incubation.

AC Broth is widely used as a pre-enrichment medium prior to plating on selective solid media and in antibiotic discovery and actinomycete ecology programs.

Key Features & Benefits

  • Highly selective enrichment for actinomycetes

  • Suppresses unwanted bacteria and fungal contaminants

  • Supports slow-growing and rare genera

  • Glycerol-based carbon source favoured by Streptomyces

  • Standard medium in modern actinomycete isolation workflows

Typical Applications

Environmental & Soil Microbiology

  • Selective enrichment of actinomycetes from soil and natural substrates

  • Recovery of actinobacteria from complex, contaminated samples

Antibiotic Discovery & Screening

  • Pre-cultivation of antibiotic-producing actinomycetes

  • Enrichment prior to plating on AC Agar or other selective agars

Taxonomy & Ecology

  • Diversity and ecological studies of actinobacteria

  • Isolation of rare genera such as:

    • Nocardia

    • Actinomadura

    • Micromonospora

Principle of the Medium

AC Broth utilises selective nitrogen sources (sodium caseinate and L-asparagine) and sodium propionate to inhibit competing microorganisms. An alkaline pH and optional antifungal agents further enhance selectivity. Glycerol, supplied separately, serves as a preferred carbon source for many actinomycetes, supporting their enrichment in liquid culture.

Composition (per litre)

  • Sodium Caseinate – 2.0 g

  • L-Asparagine – 0.1 g

  • Sodium Propionate – 4.0 g

  • Dipotassium Phosphate – 0.5 g

  • Magnesium Sulfate (MgSO₄·7H₂O) – 0.1 g

  • Ferrous Sulfate (FeSO₄·7H₂O) – 0.001 g

Final pH: 8.0–8.2 at 25 °C

Supplements (not included)

  • Glycerol – 5.0 g (≈ 5 mL)

  • Cycloheximide (Actidione) – 50 mg (filter-sterilised)

  • Nystatin (optional, for heavy fungal contamination) – 50 mg (filter-sterilised)

Preparation

  1. Suspend 11.7 g of basal medium in 1 L distilled or deionised water

  2. Adjust pH to 8.1 with 1 N NaOH if required

  3. Sterilise by autoclaving at 121 °C for 15 minutes

  4. Cool to 45–50 °C

  5. Aseptically add filter-sterilised glycerol and antibiotic solutions

  6. Dispense into sterile tubes or flasks

Incubation

  • Temperature: 25–30 °C

  • Agitation: 100–150 rpm

  • Duration: 5–14 days

  • Extended incubation (up to 4 weeks) may be required for rare or slow-growing genera

Storage & Shelf Life

  • Dehydrated medium: Store below 30 °C, dry and protected from light

  • Prepared broth (with antibiotics): Store at 2–8 °C and use within 2 weeks

Intended Use

For laboratory and research use only.
Not for diagnostic, medical, food, or veterinary applications.

Quality & Documentation

  • Manufactured under controlled conditions for batch consistency

  • Certificate of Analysis (COA) available

  • SDS available upon request

Customs & Trade Information

HS / AHECC Code: 3821.00.00
Prepared culture media for development or maintenance of microorganisms

CUSTOMERS ALSO BOUGHT