Ampicillin Sodium Salt (ASA-2044)

Ampicillin Sodium Salt (CAS 69-52-3)

Broad-Spectrum β-Lactam Antibiotic for Microbiology & Molecular Biology Research

Catalog Number: ASA-2044

Overview

Ampicillin Sodium Salt is a water-soluble, broad-spectrum β-lactam antibiotic widely used in microbiology, molecular biology, and cell culture research. It acts by inhibiting bacterial cell wall synthesis through binding to penicillin-binding proteins, leading to cell lysis in actively dividing bacteria.

The sodium salt form ensures rapid dissolution and uniform distribution in aqueous solutions, making it ideal for antibiotic-supplemented culture media, plasmid selection, and antimicrobial assays. Ampicillin remains a standard selection antibiotic for Escherichia coli and other susceptible Gram-positive and Gram-negative bacteria.

Key Features & Benefits

Broad-spectrum β-lactam antibiotic

Excellent water solubility for liquid and solid media

Reliable selection marker for plasmid-bearing strains

Suitable for routine microbiology and molecular biology workflows

Consistent research-grade purity

Typical Applications

Molecular Biology & Genetics
Plasmid selection in E. coli

Recombinant DNA cloning workflows

Maintenance of antibiotic resistance markers

Microbiology
Inhibition of susceptible Gram-positive and Gram-negative bacteria

Antimicrobial susceptibility and resistance studies

Preparation of antibiotic-supplemented media (LB, SOC, TB)

Research & Teaching Laboratories
Routine antibiotic selection experiments

Demonstration of β-lactam antibiotic mechanisms

Principle of Action

Ampicillin inhibits bacterial cell wall synthesis by interfering with peptidoglycan cross-linking. This weakens the bacterial cell wall, leading to osmotic imbalance and lysis during active growth phases.

Typical Properties

Molecular Formula: C₁₆H₁₈N₃NaO₄S

Molecular Weight: 371.39 g/mol

Appearance: White to off-white crystalline powder

Solubility: Freely soluble in water

Purity: ≥ 98 %

Storage & Stability

Store at 2–8 °C, protected from light and moisture

Shelf life: 24 months under recommended storage conditions

Prepared solutions should be stored frozen and protected from repeated freeze–thaw cycles

Intended Use

For laboratory research use only.
Not for human, veterinary, food, feed, or pharmaceutical use.

Quality & Compliance

Manufactured under controlled conditions for batch-to-batch consistency

Supplied with Certificate of Analysis (CoA) and SDS upon request

Performance comparable to leading international laboratory suppliers