Bifidobacterium Broth

Bifidobacterium Broth

Nutrient Liquid Medium for Cultivation of Bifidobacterium infantis

Catalog Number: AS-1139

Overview

Bifidobacterium Broth is a nutrient-rich liquid culture medium formulated for the cultivation and maintenance of bifidobacteria, with particular suitability for Bifidobacterium infantis. The balanced combination of peptones, yeast extract, tomato solids, glucose, and Tween 80 supplies essential amino acids, vitamins, carbohydrates, and growth factors required for robust bifidobacterial growth.

Optimised at pH 6.8 ± 0.2 and intended for use under anaerobic conditions, this broth is widely applied in clinical microbiology, probiotic quality control, fermented food testing, and gut microbiota research.

Key Features & Benefits

• Rich, complex nutrient profile for reliable bifidobacteria growth

• Tomato solids and glucose enhance recovery and metabolic activity

• Tween 80 improves membrane permeability and growth efficiency

• Optimised pH for Bifidobacterium species

• Standardised formulation for reproducible results across laboratories

Typical Applications

Probiotic & Food Microbiology

• Cultivation of Bifidobacterium infantis and related species

• Quality control of probiotic products and fermented dairy foods

Clinical & Veterinary Research

• Maintenance of bifidobacteria cultures

• Studies of intestinal microbiota composition

Gut Microbiota & Functional Foods Research

• Evaluation of bifidobacterial growth and viability

• Research into probiotic functionality and formulation

Principle of the Medium

Bifidobacterium Broth provides a reduced, nutrient-rich environment suitable for anaerobic bifidobacteria:

• Tryptone and peptone supply peptides, amino acids, and nitrogen

• Yeast extract provides vitamins, nucleotides, and trace nutrients

• Tomato juice solids contribute organic acids and additional growth factors

• Glucose serves as a readily fermentable energy source

• Tween 80 enhances nutrient uptake and cell membrane function

Together, these components support consistent growth and maintenance of bifidobacteria under anaerobic conditions.

Composition (Typical – per litre)

• Tryptone: 20.0 g

• Peptone: 10.0 g

• Yeast extract: 10.0 g

• Tomato juice solids: 16.65 g

• Dextrose (glucose): 20.0 g

• Polysorbate 80 (Tween 80): 2.0 g

Optional (for solid medium):

• Agar: 15.0 g

Final pH at 25 °C: 6.8 ± 0.2

Preparation (Typical)

• Suspend 78.65 g of dehydrated medium in 1 L purified or distilled water

• Heat gently if required to dissolve completely

• Dispense into tubes or flasks

• Sterilise by autoclaving at 121 °C for 15 minutes

• Cool and use under anaerobic conditions

Incubation

• Atmosphere: Anaerobic

• Temperature: 35–37 °C

• Incubation time: 24–72 hours, depending on strain and application

Storage & Stability

Dehydrated medium

• Store below 30 °C in a tightly closed, dry container

Prepared broth

• Store at 2–8 °C

• Use before the stated expiry date

Intended Use

For laboratory research, probiotic quality control, food microbiology, and educational use only.

Not for human or animal consumption, diagnostic, or therapeutic use.

Quality & Compliance

• Formulation aligned with ISO 29981 recommendations for bifidobacteria cultivation

• Manufactured under controlled conditions for batch consistency

• Certificate of Analysis (COA) available

• Safety Data Sheet (SDS) and Technical Data Sheet (TDS) available