Bile Peptone Transport Medium

Bile Peptone Transport Medium

Selective Transport Medium for Enteric Pathogens
Catalog Number: AS-1142

Overview

Bile Peptone Transport Medium is a selective, non-nutritive transport medium designed for the preservation and transport of enteric bacteria, particularly Salmonella spp., from food, water, environmental, and clinical samples to the laboratory.

The formulation contains bile salts to suppress Gram-positive organisms and competing background flora, while peptone maintains bacterial viability without supporting multiplication. This ensures that target organisms remain viable during transport while preventing overgrowth and changes in the original microbial population.

Bile Peptone Transport Medium is commonly used as a holding medium prior to selective enrichment or plating, making it an essential component of food safety, water testing, and public-health microbiology workflows.

Applications

• Transport of Salmonella spp. from food, feed, and environmental samples

• Preservation of enteric pathogens during sample transit

• Holding medium prior to selective enrichment (RV, SC, Tetrathionate)

• Food safety, water microbiology, and environmental monitoring

Key Features

• Selective bile salts inhibit Gram-positive bacteria

• Maintains organism viability without allowing growth

• Prevents overgrowth during transport

• Simple, stable formulation suitable for routine field sampling

• Fully compatible with downstream enrichment and selective plating media

Principle of the Medium

• Bile salts inhibit Gram-positive organisms and non-target flora

• Peptone provides minimal nutrients to maintain cell integrity

• Non-nutritive design preserves the original microbial load

This ensures accurate recovery of enteric pathogens during laboratory analysis.

Composition (per litre)

• Peptone: 10.0 g

• Bile salts: 5.0 g

• Sodium chloride: 5.0 g

Final pH at 25 °C: 7.4 ± 0.2

Preparation

• Suspend 20.0 g of dehydrated medium in 1 L purified water

• Heat gently with agitation until fully dissolved

• Dispense into sterile transport tubes or bottles

• Sterilize by autoclaving at 121 °C for 15 minutes

• Cool to room temperature before use

Storage & Stability

• Dehydrated medium: store below 30 °C in a cool, dry place

• Prepared medium: store at 2–8 °C

• Shelf life: refer to product label

Intended Use

For laboratory food, water, environmental, and research microbiology use only.
Not intended for therapeutic or diagnostic use.

Quality & Compliance

• Manufactured under controlled conditions for batch consistency

• Suitable for ISO and FDA-aligned food microbiology workflows

• Certificate of Analysis (COA) available

• Safety Data Sheet (SDS) available upon request