Brucella Broth | Enriched Liquid Medium for Brucella Cultivation | AS‑1156

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AuSaMicS Life Science • Culture Media

Brucella Broth

Enriched broth for Brucella spp., anaerobes, and other fastidious microorganisms

Highly nutritious liquid medium formulated for the cultivation, enrichment, and maintenance of Brucella species and a wide range of fastidious, anaerobic, and microaerophilic bacteria. Brucella Broth provides a rich digest-based nutrient system and is commonly used alone or with blood, serum, vitamin K, hemin, or selective supplements for demanding microbiology workflows.

Cat. No: AS-1156 Enriched Broth Base ✓ Australian Stock 🧫 Fastidious Recovery
🚀 Fast Dispatch: Australian supply 🧪 Microbiological Grade: High-nutrient formulation 📋 Documentation: COA + SDS available

🏆 Rich Broth for Demanding Organisms

🧫 High Nutrient Density - supports fastidious growth
🌫️ Anaerobe Compatible - suitable for reduced-oxygen workflows
🩸 Supplement Friendly - accepts blood and growth factors
🔬 Flexible Use - enrichment, recovery, maintenance
📞 Technical Support - method and workflow guidance
Broth Enrichment Ready
Frequently used for enrichment and propagation of delicate isolates

🔬 Product Overview

Brucella Broth is a rich, non-selective liquid medium developed for the cultivation of Brucella spp. and numerous other fastidious, anaerobic, and microaerophilic organisms. Its digest-rich composition provides amino acids, vitamins, and energy sources required for reliable recovery of delicate or nutritionally demanding bacteria.

The broth is widely used in diagnostic microbiology, veterinary bacteriology, anaerobe culture, and research laboratories as a primary enrichment medium or as a base for supplementation with blood, serum, vitamin K, hemin, and selective antimicrobials, depending on the intended workflow.

⚗️ Principle of the Medium

Peptones + digests + dextrose + yeast extract

Rich liquid nutrient environment

Supports enrichment and propagation of fastidious bacteria

Can be further enhanced with blood, serum, vitamin K, hemin, or selective agents

Brucella Broth supplies a highly supportive nutrient base that promotes growth of organisms with demanding requirements. In appropriately controlled atmospheres, it performs well for the enrichment and recovery of Brucella-related isolates, anaerobes, and other delicate bacteria that may grow poorly in simpler broths.

🧬 Key Applications

🦠 Brucella Enrichment

Used for cultivation and enrichment of Brucella species under suitable laboratory conditions and biosafety procedures.

🌫️ Anaerobic Microbiology

Suitable as a broth base for enrichment and maintenance of anaerobic bacteria when used with appropriate supplements and atmospheric control.

🏥 Clinical & Veterinary Support

Useful in laboratories handling fastidious organisms from clinical, veterinary, or environmental specimens.

🧪 Supplemented Broth Systems

Ideal as a flexible liquid base for preparing blood-enriched or selectively supplemented broth systems tailored to specific methods.

✨ Key Features

  • Rich digest-based broth for demanding organisms
  • Suitable for enrichment, propagation, and maintenance
  • Compatible with blood and serum supplementation
  • Useful in anaerobic and microaerophilic workflows
  • Supports Brucella-related laboratory methods
  • Available with COA and SDS support

🧪 Typical Composition (g/L)

Component Approx. Role Function
Peptones / enzymatic digests Primary nutrient base Provide nitrogen, peptides, and amino acids
Yeast extract Growth factor source Supplies vitamins and accessory nutrients
Dextrose Energy source Supports active growth and enrichment
Sodium chloride Osmotic balance Maintains suitable broth conditions
Buffering salts Medium stability Help maintain pH during incubation

Exact formulation may vary slightly according to manufacturing standard and validated performance requirements.

📊 Related Enriched Media Comparison

Medium Type Main Use Typical Role
Brucella Broth Enriched broth base Liquid enrichment of Brucella and fastidious organisms Primary broth enrichment
Brucella Agar Enriched agar base Isolation and subculture of fastidious organisms Solid recovery medium
Thioglycollate Medium Reducing enrichment broth Growth of aerobes and anaerobes in gradient conditions General broth recovery
Brain Heart Infusion Broth Rich nutritive broth General fastidious bacterial cultivation Broad enriched broth use

💡 Preparation & Use Guidance

Use Case:
For enrichment and cultivation of fastidious and anaerobic bacteria
Supplementation:
May be used with blood, serum, vitamin K, hemin, or selective additives as required
Atmosphere:
Apply the appropriate incubation atmosphere for the target organism
Storage:
Store dehydrated medium tightly closed in a cool, dry place

📋 Technical Specifications

Product Name Brucella Broth
Catalog Number AS-1156
Format Dehydrated powder
Medium Type Enriched broth base
Primary Function Cultivation and enrichment of fastidious bacteria
Typical Supplementation Blood, serum, vitamin K, hemin, selective additives
Appearance Light beige to tan powder
Prepared Medium Amber to light brown broth
Storage Keep tightly closed in a dry place, protected from moisture and light

🎯 Typical Organism Scope

  • Brucella spp.
  • Anaerobic bacteria
  • Microaerophilic organisms
  • Other fastidious isolates

✅ Quality & Documentation

  • Microbiological grade raw materials
  • Batch QC documentation
  • COA available
  • SDS support

📄 Available Documents

Certificate of Analysis (COA)
Safety Data Sheet (SDS)
Technical Data Sheet (TDS)

🧫 Related AuSaMicS Media

Brucella Agar

Solid enriched counterpart

View Product
Thioglycollate Medium

Reducing broth system

View Product
Brain Heart Infusion Broth

Rich general fastidious broth

View Product
Anaerobe Media Range

Broths and agars for reduced-oxygen workflows

View Collection

Need a complete fastidious or anaerobe broth workflow?

AuSaMicS can support enriched broths, agar companions, supplements, and technical documentation for demanding microbiology applications.

For laboratory and research use only. Use according to validated laboratory methods and internal quality procedures.

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