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Charcoal Agar (AS-1172) | Specialized Medium for Bordetella Isolation
$87.00 AUD
AuSaMicS Life Science • Fastidious Pathogen Recovery
Charcoal Agar
Bordetella Isolation & Maintenance Medium
Specialized neutralizing medium for the cultivation of Bordetella pertussis and Haemophilus. Formulated with activated charcoal to absorb inhibitory metabolic by-products and toxic substances.
AS-1172
🌑 Neutralizing Matrix
✓ Blood-Free Performance
🔬 Clinical Grade
Dispatch: Australian Stock Standard: ISO 11133 Compliant Prep: 41g/L formulation
Respiratory Pathogen Specialist
Detoxification - Neutralizes fatty acids
Bordetella Focus - Optimized for Pertussis
Transport Stability - High survival rates
Visual Contrast - Opaque black background
Activated Charcoal
4.0g/L High-Absorption Tech
🧪 Complete Formulation & Neutralizing Profile
📋 Ingredient Composition (per Liter)
⚗️ Detoxification & Growth System
Toxin Absorption
Charcoal removes fatty acids toxic to Bordetella
Charcoal removes fatty acids toxic to Bordetella
Starch Replacement
Activated carbon outperforms traditional starch agar
Activated carbon outperforms traditional starch agar
Enhanced Contrast
Black base provides superior colony visualization
Black base provides superior colony visualization
Respiratory Media Comparison
| Medium | Neutralizer | Target | Key Advantage |
|---|---|---|---|
| Charcoal Agar (AS-1172) | Activated Carbon | Bordetella / Haemophilus | Long survival & contrast |
| Bordet-Gengou Agar | Potato Starch | B. pertussis | Hemolysis detection |
| Chocolate II Agar | Corn Starch | Fastidious Pathogens | Factor X/V Availability |
🔬 Quality Control & Performance Standards
Growth Performance:
B. pertussis ATCC 9340
"Mercury drop" colonies
B. pertussis ATCC 9340
"Mercury drop" colonies
Secondary Check:
H. influenzae ATCC 19418
Typical smooth colonies
H. influenzae ATCC 19418
Typical smooth colonies
Physical Prop:
pH 7.3 ± 0.2
Opaque black, firm gel
pH 7.3 ± 0.2
Opaque black, firm gel
📋 Technical Specifications
| Catalog No. | AS-1172 |
| Weight/Liter | 41.0 g/L |
| Sterilization | 121°C, 15 min |
| Shelf Life | 3 years |
🦠 Supported Pathogens
- ✓ Bordetella pertussis
- ✓ Bordetella parapertussis
- ✓ Haemophilus influenzae
- ✓ Neisseria spp.
⚗️ Preparation Protocol
- 1 Suspend 41g in 1L purified water.
- 2 Boil with agitation until fully dissolved.
- 3 Autoclave at 121°C for 15 min.
- 4 Important: Swirl during pouring to ensure even charcoal distribution.
📦 Storage
Store dehydrated media at 15-30°C. Prepared plates are stable at 2-8°C for up to 4 weeks if protected from evaporation.