Phyto Casein Hydrolysate Yeast Extract Agar Base (D2ANX Medium)
Phyto Casein Hydrolysate Yeast Extract Agar Base (D2ANX Medium) - AS-1394
🧬 Enriched Plant Pathology Medium
Premium enriched culture medium with casein hydrolysate and yeast extract, specifically formulated for isolation and cultivation of fastidious plant pathogenic bacteria requiring enhanced nutritional support.
🔬 Technical Overview & Biochemistry
D2ANX Medium represents an advanced nutritional platform combining enzymatic casein hydrolysate with yeast extract to provide comprehensive amino acid profiles, vitamins, and growth factors essential for fastidious plant pathogenic bacteria. The medium's enriched formulation supports primary isolation from challenging plant tissue samples where standard media may fail.
Nutritional Enhancement: The synergistic combination of casein hydrolysate (providing peptides and amino acids) with yeast extract (supplying B-vitamins, trace elements, and nucleotides) creates an optimal growth environment for nutritionally demanding plant pathogens, particularly those with complex metabolic requirements.
Key Features & Applications
🧪 Enriched Formulation
High-quality casein hydrolysate and yeast extract provide comprehensive nutritional support for fastidious plant pathogenic bacteria.
🌱 Primary Isolation
Optimized for direct isolation from plant tissues, seeds, and environmental samples with challenging bacterial populations.
📊 Enhanced Recovery
Superior growth support for nutritionally demanding plant pathogens with improved isolation success rates.
🔬 Research Applications
Ideal for phytobacteriology research, strain maintenance, and physiological studies of plant pathogenic bacteria.
Cross-Reference / Equivalent Products
| Manufacturer | Product Name | Catalogue Number | Notes |
|---|---|---|---|
| Oxoid | Casein Peptone Yeast Extract Agar | CM0397 | General purpose, less enriched |
| BD Difco | Yeast Extract Agar | 212750 | Similar base, different formulation |
| Merck | Casein Peptone Yeast Extract Agar | 105458 | Standard formulation |
| Thermo Fisher | Tryptic Soy Agar + YE | R455052 | Alternative enriched medium |
🦠 Target Applications & Organisms
- Fastidious Plant Pathogenic Bacteria: Nutritionally demanding species requiring enriched media
- Primary Isolation: Direct isolation from infected plant tissues and seeds
- Strain Maintenance: Long-term cultivation and preservation of plant pathogenic bacteria
- Physiological Studies: Research applications requiring optimal nutritional conditions
- Diagnostic Applications: Enhanced recovery in plant disease diagnosis
🔍 Related Search Terms
Common searches: enriched plant pathology medium, casein hydrolysate yeast extract agar, fastidious plant pathogen isolation, phytobacteriology culture media, D2 medium plant pathology, enriched bacterial isolation medium, plant pathogenic bacteria culture, diagnostic microbiology media, agricultural pathology supplies
Technical Specifications
| Parameter | Specification | Method |
|---|---|---|
| pH (25°C) | 7.2 ± 0.2 | Potentiometric |
| Appearance | Light beige, translucent gel | Visual inspection |
| Total Nitrogen | ≥2.5% | Kjeldahl method |
| Amino Nitrogen | ≥0.8% | Formol titration |
| Preparation | 43.0g per 1000mL | Standard protocol |
| Shelf Life | 36 months | Stability testing |
Preparation Instructions
- Hydration: Suspend 43.0g in 1000mL distilled water
- Mixing: Mix thoroughly and heat with frequent agitation
- Dissolution: Boil for 1 minute until completely dissolved
- Sterilization: Autoclave at 121°C for 15 minutes
- Cooling & Pouring: Cool to 50-55°C before pouring plates
- Storage: Store prepared plates at 2-8°C, use within 4 weeks
Technical Data Sheet - D2ANX Medium
Complete technical specifications, composition analysis, and validation protocols
The Technical Data Sheet provides comprehensive information including:
- Detailed compositional analysis and nutritional profile
- Performance validation with reference strains and plant pathogens
- Quality control specifications and analytical test methods
- Literature references and application protocols for plant pathology
- Regulatory compliance and manufacturing standards
Safety Data Sheet - D2ANX Medium
Complete safety information and handling procedures (16-section GHS format)
The Safety Data Sheet includes:
- Hazard identification and GHS classification
- Safe handling, storage, and disposal procedures
- Emergency response and first aid measures
- Personal protective equipment recommendations
- Regulatory compliance information for Australia and export
Classification: Not classified as hazardous according to Australian WHS regulations. Standard laboratory safety practices recommended.
Certificate of Analysis - D2ANX Medium
Batch-specific quality control results and performance certification
Each Certificate of Analysis provides:
- Batch-specific test results for all physical and chemical parameters
- Microbiological performance validation with reference organisms
- pH measurement and appearance verification post-sterilization
- Nutritional analysis including total and amino nitrogen content
- Quality assurance certification and batch release authorization
QC Testing: Every batch performance tested with standardized bacterial strains and subjected to comprehensive analytical verification.