Dextrose Tryptone Broth (AS-1205) | Detect "Flat-Sour" Spoilage

$75.00 AUD

AuSaMicS Life Science • Food & Beverage Quality

Dextrose Tryptone Broth

Detection of "Flat-Sour" Spoilage Bacteria

A specialized liquid medium designed for the cultivation and enumeration of aciduric and thermophilic organisms, specifically those responsible for flat-sour spoilage in canned food products.

AS-1205 🥫 Canning Industry Standard ✓ APHA / NCA 🔥 Thermophile Recovery

Target: Bacillus coagulans Indicator: Bromocresol Purple Carbohydrate: Dextrose

Diagnostic Clarity

Visual Detection - Rapid pH color shift

Rich Nutrients - Supports fastidious spores

Thermophilic Focus - Optimized for heat-stable flora

Reliable - Consistent results in low-acid foods

Indicator Range

Purple (pH 6.8) → Yellow (pH 5.2)

🔬 "Flat-Sour" Reaction Mechanism

Thermophilic "flat-sour" bacteria ferment the dextrose in this medium, producing acid without gas. The Bromocresol Purple indicator shifts from purple to yellow as the pH drops, allowing for clear visual identification of spoilage organisms like Bacillus coagulans in samples that otherwise appear physically unchanged (flat cans).

🧪 Nutrient Formulation

Ingredient	Concentration	Function
Tryptone (Casein Digest)	10.0 g	Amino Acids & Nitrogen Source
Dextrose	5.0 g	Fermentable Carbohydrate
Bromocresol Purple	0.04 g	pH Sensitivity Indicator

📋 Technical Specifications

Cat Number	AS-1205
Final pH	6.7 ± 0.2
Yield	15.04g per Liter
Storage	10-30°C

⚗️ Preparation Guide

1 Dissolve 15.04g in 1L distilled water.
2 Mix well and dissolve by heating if necessary.
3 Autoclave at 121°C for 15 minutes.
4 For thermophiles, incubate at 55°C.