Skip to product information
Request a Quote
Differential Reinforced Clostridial Medium (DRCM) | Anaerobic Bacteria Differentiation | AS‑1209
$135.00 AUD
AuSaMicS Life Science • Culture Media
Differential Reinforced Clostridial Medium
Enrichment and differentiation support medium for anaerobic Clostridia
Differential Reinforced Clostridial Medium (DRCM) is a semi-solid, nutrient-rich medium designed for the cultivation, enrichment, and differentiation support of Clostridium species and other anaerobes from food, pharmaceutical, environmental, and clinical-adjacent samples. The formulation combines peptones, beef/yeast extracts, glucose, soluble starch, sodium acetate, and L-cysteine to promote robust anaerobic growth while maintaining a low redox potential and buffering capacity.
Cat. No: AS-1209
Differential Enrichment Medium
✓ Australian Stock
🧪 Anaerobic Clostridia Medium
🚀 Fast Dispatch: Australian supply
🧪 Microbiological Grade: Routine laboratory use
📋 Documentation: COA + SDS available
🏆 Robust Anaerobic Enrichment
🧬 Clostridia Growth – supports low-inoculum recovery
🌑 Reduced Environment – L-cysteine + low agar for anaerobiosis
🔬 Differential Use – turbidity, gas, and growth pattern screening
🍽️ Food & Pharma Labs – harmonised-method fit
📞 Technical Support – method alignment assistance
Semi-solid Anaerobic System
Designed for enriched recovery and differentiation support of Clostridia in mixed samples
🔬 Product Overview
Differential Reinforced Clostridial Medium (DRCM) is a semi-solid, reinforced medium formulated for the cultivation and enumeration of Clostridium species and other anaerobes. The rich nutrient base and reducing system support growth from small inocula and help achieve high viable counts in demanding matrices.
It is suitable for routine microbiology laboratories performing anaerobic enrichment, mesophilic spore counts, and preliminary differentiation of Clostridia from food, dairy, pharmaceutical, environmental, and general laboratory samples.
⚗️ Principle of the Medium
Peptones, beef & yeast extract
↓
Rich nutrient supply
↓
Glucose & starch + sodium acetate
↓
Energy, buffering & detoxification
↓
L-cysteine + low agar
↓
Reduced redox potential for anaerobic growth
↓
Rich nutrient supply
↓
Glucose & starch + sodium acetate
↓
Energy, buffering & detoxification
↓
L-cysteine + low agar
↓
Reduced redox potential for anaerobic growth
Peptone, beef extract, and yeast extract provide carbon, nitrogen, amino acids, and B vitamins essential for anaerobe growth. Glucose serves as a fermentable carbohydrate, while soluble starch helps neutralise toxic metabolites. Sodium acetate contributes buffering capacity, and sodium chloride maintains osmotic balance. L-cysteine hydrochloride acts as a reducing agent, and the low agar content decreases oxygen diffusion, creating a favourable environment for Clostridia and other anaerobes.
🧬 Key Applications
🦠 Clostridia Enrichment
Recommended for enrichment and enumeration of Clostridium spp. and other anaerobes from complex samples.
🍽️ Food & Dairy Microbiology
Used for mesophilic anaerobic spore counts and Clostridia studies in milk, cheese, and other foods.
🏭 Pharmaceutical & Non-sterile Products
Applicable as an enrichment medium for Clostridia from non-sterile pharmaceutical products and raw materials.
🔍 Preliminary Differentiation Support
Supports presumptive differentiation based on anaerobic growth, turbidity, gas production, and subsequent subculture.
✨ Key Features
- Reinforced nutrient base for fastidious anaerobes
- L-cysteine-reduced system for low redox potential
- Semi-solid format to limit oxygen penetration
- Suitable for food, dairy, pharma, and environmental workflows
- Supports high viable counts from small inocula
- Available with COA and SDS support
🧪 Typical Composition (g/L)
Exact formulation may vary slightly by manufacturing standard and validated performance requirements.
📊 Related Anaerobic Media Comparison
💡 Preparation & Use Guidance
Use Case:
For enrichment and differentiation support of Clostridia and other anaerobes
For enrichment and differentiation support of Clostridia and other anaerobes
Reading:
Observe turbidity, gas production, and growth characteristics after anaerobic incubation
Observe turbidity, gas production, and growth characteristics after anaerobic incubation
Next Step:
Subculture onto selective/differential agar and perform biochemical confirmation
Subculture onto selective/differential agar and perform biochemical confirmation
Storage:
Store dehydrated medium tightly closed in a cool, dry place, protected from light
Store dehydrated medium tightly closed in a cool, dry place, protected from light
📋 Technical Specifications
| Product Name | Differential Reinforced Clostridial Medium |
| Abbreviation | DRCM |
| Catalog Number | AS-1209 |
| Format | Dehydrated powder |
| Medium Type | Semi-solid anaerobic enrichment medium |
| Primary Function | Cultivation and differentiation support of Clostridia and other anaerobes |
| Appearance | Light beige to tan, free-flowing powder |
| Prepared Medium | Yellow to amber, semi-solid medium |
| Storage | Keep tightly closed in a dry place, protected from moisture and light |
| Application | Food, dairy, pharmaceutical, environmental, and routine anaerobic microbiology |
🎯 Typical Organism Scope
- ✓ Clostridium species (e.g. C. perfringens, C. sporogenes)
- ✓ Other anaerobic bacteria from food and environmental samples
- ✓ Mesophilic anaerobic spore-formers
- ✓ Facultative anaerobes under anaerobic incubation
✅ Quality & Documentation
- ✓ Microbiological grade raw materials
- ✓ Batch QC documentation
- ✓ COA available
- ✓ SDS support
📄 Available Documents
Certificate of Analysis (COA)
Safety Data Sheet (SDS)
Technical Data Sheet (TDS)
🧫 Related AuSaMicS Media
Need a complete anaerobic Clostridia workflow?
AuSaMicS can support enrichment media, plating media, and technical documentation for food, pharma, and routine anaerobic workflows.
For laboratory and research use only. Use according to validated laboratory methods and your internal quality procedures.