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E. coli Cloning & Blue-White Screening Media Set
$260.00 AUD
E. coli Cloning Media — Australian Made
E. coli Cloning & Blue-White Screening Media Set
A coordinated set of culture media and screening reagents for commonly used E. coli cloning, transformation and blue-white screening workflows. Antibiotic selection is configurable to your vector's resistance marker. Full documentation on every item.
Manufactured in Melbourne Fast Australian dispatch SDS · TDS · COA per product LOT & EXP traceability Direct formulator support
Where each product fits in the workflow
1. TransformationCompetent cells + plasmid
→
2. RecoverySOC Medium (optional)
→
3. Selection/PlatingLB Agar + chosen antibiotic
→
4. Blue-white screeningIPTG + X-Gal
→
5. CultureLB Broth
E. coli Cloning & Blue-White Screening Media Set
Culture media and screening reagents for routine E. coli cloning, transformation and blue-white screening. Choose the selection antibiotic that matches your vector's resistance marker, and add SOC Medium if your protocol requires a recovery step.
$260 Core set · Save $14 vs $274 individually
Configured total: $287
Core set — included
- LB Agar (Lennox) 500 g — AS-1268
- LB Broth (Lennox) 500 g — AS-1269
- IPTG 1 g — ASE-901-1
- X-Gal 1 g — ASE-906-1G
Blue-white screening requires a compatible lacZ-based host/vector system. IPTG and X-Gal alone do not produce blue-white screening in an incompatible system. Confirm that your host strain and cloning vector support α-complementation before use.
Choose your selection antibiotic
Select the antibiotic that matches your vector's resistance marker.
Selection depends on the resistance marker carried by your vector/plasmid — check your vector map before ordering.
Optional transformation recovery
Add Cloning Media Set — $287
Need different pack sizes or quantities? Configure this set for your laboratory →
Colour key: Culture media Screening substrates
Exact products & quantities — core set
| Product | Code | Pack size |
|---|---|---|
| LB Agar (Lennox) | AS-1268 | 500 g |
| LB Broth (Lennox) | AS-1269 | 500 g |
| IPTG | ASE-901-1 | 1 g |
| X-Gal | ASE-906-1G | 1 g |
Selection antibiotic and SOC Medium are configured above and added as separate line items. LB Miller formulation (AS-1267, AS-1270) is available individually if preferred over Lennox — contact support@ausamics.com. Bulk pack sizes and custom quantities available on request.
High-density culture & expression
High-Yield Media
Terrific Broth (TB)
AS-1429
Phosphate-buffered medium supporting high-density E. coli culture, plasmid propagation, and recombinant protein expression workflows.
$89 500 g
Add Terrific Broth →
Phage & high-density culture
High-Density Media
2×YT Broth
AS-1398
Double-strength yeast-tryptone broth for phage display, antibody library construction, and high-density pre-culture.
$95 500 g
Add 2×YT Broth →
Solid Media
2×YT Agar
AS-1395
Double-strength yeast-tryptone agar for high-efficiency plating of phage display libraries and high-density E. coli colonies.
$95 500 g
Add 2×YT Agar →
Downstream DNA analysis
Gel Electrophoresis
Agarose — Mol. Bio. Grade
AS-1015
For agarose gel electrophoresis following colony PCR, restriction digest, or plasmid size analysis. Low EEO for sharp band resolution.
$79 50 g
Add Agarose →
β-galactosidase assays
Screening Substrate
ONPG
ASE-904
o-Nitrophenyl-β-D-galactopyranoside — colorimetric substrate for quantitative β-galactosidase activity assays, used in lacZ reporter studies and Miller unit calculations.
$29 1 g
Add ONPG →
Custom media formulation
Base Component
Bacteriological Peptone
AS-1011
For laboratories preparing their own LB or other bacterial media from base components rather than pre-formulated dehydrated media.
$29 100 g (500 g / 1 kg / 5 kg also available)
Add Peptone →
Base Component
Yeast Extract
AS-1010
B-vitamins and accessory nutrients for custom media preparation alongside peptone and agar.
$25 100 g (500 g / 1 kg also available)
Add Yeast Extract →
Base Component
Bacteriological Agar (Agar-Agar)
AS-1001
Solidifying agent for custom-formulated solid media, for laboratories that prepare plates from individual components.
$29 100 g (500 g / 1 kg also available)
Add Agar →
Why AuSaMicS E. coli cloning media?
Australian-manufacturedCulture media manufactured locally in Melbourne for faster supply and reduced reliance on imported finished media.
Fast Australian dispatchManufactured in Melbourne and dispatched promptly following production and batch release.
Full documentationSDS, TDS and batch-specific COA provided for each product in the set.
LOT & EXP traceabilityBatch-coded to support laboratory traceability, documentation and audit requirements.
One order, coordinated setCore cloning media sourced together, with antibiotic and recovery medium configured to your protocol.
Direct formulator supportSpeak directly with the chemist. Protocol guidance and custom quantities available on request.
Frequently asked questions
Q1: What is included in the E. coli Cloning & Blue-White Screening Media Set?
The core set is LB Agar (Lennox) 500 g, LB Broth (Lennox) 500 g, IPTG 1 g, and X-Gal 1 g — the media and screening substrates common to most standard cloning and blue-white screening workflows. Your selection antibiotic and, if needed, SOC Medium are configured separately above and added as their own line items.
Q2: Which antibiotic should I choose?
Selection depends on the resistance marker carried by your vector or plasmid — not on the cloning workflow itself. Check your vector map or plasmid documentation and use the selection conditions specified by the applicable protocol.
Q3: Do I need SOC Medium?
SOC Medium is used for the post-transformation recovery step — the incubation period immediately after heat shock or electroporation, before plating. It's not required for every protocol; some labs use LB for recovery instead. Add it if your transformation protocol calls for a dedicated rich recovery medium.
Q4: What are IPTG and X-Gal used for?
IPTG is a non-metabolisable inducer of lac-controlled expression, while X-Gal is a chromogenic β-galactosidase substrate. Together they enable blue-white colony screening in a compatible host/vector system with an appropriate lacZ α-complementation system. Confirm that your strain and vector support this before relying on colony colour for screening.
Q5: What's the difference between LB Lennox and LB Miller formulations?
LB Lennox contains 5 g/L NaCl, while LB Miller contains 10 g/L NaCl. Both support E. coli growth; Lennox is the formulation used in this set by default. Miller versions (AS-1267, AS-1270) are available individually if your protocol specifies it.
Q6: Can I order different pack sizes or quantities?
Yes — all products in this set are available individually and in bulk pack sizes. For custom configurations or larger quantities, use the "Configure this set for your laboratory" link above or contact support@ausamics.com.
E. coli Cloning & Blue-White Screening Media Set
LB Agar · LB Broth · IPTG · X-Gal — plus your chosen antibiotic and optional SOC Medium
Add Cloning Media Set — $287
Research Use Only. Products in this set are supplied for in vitro laboratory research by appropriately trained personnel. Selection antibiotics and screening reagents should be used according to the applicable vector, host-strain and institutional protocols — consult individual SDS and product documentation before use. Full terms and liability information: ausamics.com.au/policies/terms-of-service.