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mEC Broth | Modified EC Broth with Novobiocin
  • mEC Broth | Modified EC Broth with Novobiocin
mEC Broth | Modified EC Broth with Novobiocin | AS-1213 | AuSaMicS

mEC Broth | Modified EC Broth with Novobiocin | AS-1213 | AuSaMicS

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EMPTY DOM REMOVE PROTECTOR
AuSaMicS Life Science • Selective Enrichment

mEC Broth

Modified EC Broth with Novobiocin

Premium selective enrichment medium for E. coli O157:H7 detection. Enhanced with Novobiocin for >95% suppression of Gram-positive interference. Gold standard for food safety and pathogen surveillance programs.

AS-1213 O157:H7 ✓ Australian Stock ⚠️ Selective
Same-Day Dispatch: Australian Stock USDA-FSIS Grade: Validated protocol Pathogen Enrichment: BSL-2 conditions

 O157:H7 Specialist

Pathogen Expert - EHEC enrichment
Food Safety - Meat & dairy testing
Complete Package - COA + BSL protocols
Fast Supply - Ships within 24h
Expert Support - Pathogen method validation
Novobiocin Enhanced
95% Gram-positive suppression

 Complete Formulation & Selectivity Mechanism

📋 Ingredient Composition (per Liter)

Ingredient Concentration Primary Function Mechanism
Peptone 20.0 g Nitrogen source Casein hydrolysate, amino acids
Lactose 5.0 g Fermentation substrate Gas production, E. coli detection
Sodium Chloride 5.0 g Osmotic balance Maintains cell integrity
Bile Salts No. 3 1.12 g Selective agent Inhibits Gram-positive bacteria
Phosphates (Buffered) 5.50 g pH stabilization Prevents acid-induced cell death
Novobiocin 20 mg/L Enhanced selectivity DNA gyrase inhibition, Gram-positive suppression

 Triple Selectivity Enhancement

1. Bile Salt Selection
Disrupts Gram-positive cell membranes
2. Novobiocin Enhancement
DNA gyrase inhibition, >95% suppression
3. Lactose Fermentation
Gas production confirms E. coli
Critical Enhancement: Novobiocin at 20 mg/L provides superior selectivity for O157:H7 isolation from complex food matrices with heavy Gram-positive contamination

📊 Complete E. coli O157:H7 Enrichment Portfolio

Medium Selectivity Level O157:H7 Recovery Background Suppression Key Advantage Standards
mEC Broth (Novobiocin) (AS-1213) ⭐⭐⭐⭐⭐ Excellent 92-97% 95% Gram-positive Superior meat & dairy performance USDA-FSIS, ISO 16654
Standard EC Broth (AS-1214) ⭐⭐⭐ Moderate 78-88% 70% Gram-positive Water quality, general coliforms APHA Standard Methods
TSA Broth (AS-1371) + Vancomycin (ASA-2025) ⭐⭐⭐⭐ High 85-92% 85% Gram-positive Alternative selective enrichment Research methods
GN Broth (AS-1244) ⭐⭐⭐ Moderate 70-82% 80% Gram-positive Broad Gram-negative enrichment Clinical microbiology
Lauryl Tryptose Broth (AS-1266) ⭐⭐ Low 65-78% 40% Gram-positive Gentle on stressed cells Presumptive coliform
EE Broth (AS-1216) ⭐⭐⭐ Moderate 75-85% 60% Gram-positive Broad Enterobacteriaceae European methods

 Strategic Method Selection for O157:H7 Detection

✅ mEC Broth Advantages

  • ✓ Highest Recovery: 92-97% for O157:H7
  • ✓ Superior Selectivity: >95% Gram-positive suppression
  • ✓ Food Matrix Optimized: Meat & dairy products
  • ✓ Regulatory Validated: USDA-FSIS approved
  • ✓ pH Buffered: Prevents acid-induced death
  • ✓ Pathogen Focus: EHEC detection optimized

⚠️ mEC Considerations

  • ▲ BSL-2 Required: Pathogen enrichment protocols
  • ▲ Novobiocin Critical: Lot-specific QC needed
  • ▲ Confirmation Required: SMAC plating necessary
  • ▲ Higher Cost: vs. standard EC broth
  • ▲ Storage Sensitive: Prepared medium 2 weeks max

🎯 Application-Specific Selection

  • ◆ Ground Beef: mEC Broth (heavy Gram-positive flora)
  • ◆ Raw Milk: mEC Broth (enterococci interference)
  • ◆ Water Quality: Standard EC (general coliforms)
  • ◆ Produce: TSA+Vancomycin (alternative method)
  • ◆ Clinical Isolates: GN Broth (broad spectrum)

 Sample-Specific O157:H7 Recovery Rates

Sample Matrix mEC Broth Standard EC Key Challenge
Ground Beef (80/20) 92-97% 65-78% High Gram-positive background
Raw Milk 88-94% 58-72% Enterococci interference
Fresh Produce 85-92% 75-85% Environmental bacteria
Poultry (Carcass rinse) 90-95% 68-80% Multiple pathogen types
Cheese (Soft) 87-93% 55-68% Lactic acid bacteria
Environmental Samples 82-88% 75-82% Diverse microbial community

🔬 O157:H7 Detection Protocol & Quality Control

Enrichment Protocol:
25g sample + 225mL mEC
35-37°C, 24-48 hours
Positive Control:
E. coli O157:H7 ATCC 43888
Gas production + turbidity
Negative Control:
Enterococcus faecalis
No growth (suppressed)
Confirmation:
SMAC agar plating
Sorbitol-negative colonies
⚠️ BSL-2 Safety Requirements: Handle under appropriate biosafety conditions. This medium enriches for human pathogens. Use universal precautions and autoclave all waste.

📋 Technical Specifications

Catalogue Number AS-1213
Dehydrated Weight 36.62 g/L
Final pH 6.9 ± 0.2 (at 25°C)
Novobiocin Level 20 mg/L
Appearance (Powder) Light cream to beige
Prepared Medium Light amber, clear broth
Sterilization 121°C, 15 minutes
Incubation 35-37°C, 24-48 hours
Shelf Life 3 years

 Target Pathogens

  • E. coli O157:H7 - Primary EHEC strain
  • E. coli O26 - Non-O157 STEC
  • E. coli O103 - Emerging strain
  • E. coli O111 - Clinical significance
  • Fecal Coliforms - General detection

📜 Regulatory Compliance

  • ✓ USDA-FSIS MLG 5C - O157:H7 detection
  • ✓ ISO 16654:2001 - EHEC detection
  • ✓ FDA BAM Chapter 4A - E. coli methods
  • ✓ Health Canada MFHPB-30 - O157 methods
  • ✓ AOAC Official Method 995.14 - Validated

⚠️ Critical Success Factors

  • Temperature: 35-37°C ± 1°C precision
  • Timing: Check at 18-24h and 48h
  • Durham Tubes: Inverted for gas detection
  • Novobiocin QC: Lot-specific validation
  • BSL-2: Pathogen safety protocols

✅ Quality Standards

  • ✓ Performance Testing: O157:H7 validation
  • ✓ Selectivity Testing: Gram-positive suppression
  • ✓ pH Verification: 6.9 ± 0.2 confirmed
  • ✓ Sterility Testing: USP <71> compliant
  • ✓ BSL Documentation: Safety protocols included

 Complete O157:H7 Detection System

Enrichment & Supporting Media

Standard EC Broth (AS-1214)

General coliform enrichment

View Product
Lauryl Tryptose Broth (AS-1266)

Presumptive coliform

View Product
TSA (AS-1370) + Vancomycin (ASA-2025)

Alternative selective broth

View Product
EE Broth (AS-1216)

Enterobacteriaceae enrichment

View Product

Selective Plating Media

SMAC Agar

Sorbitol MacConkey

View Product
CT-SMAC Agar

Cefixime-tellurite enhanced

View Product
Rainbow Agar O157

Chromogenic detection

View Product
MacConkey Agar

General selective plating

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Confirmation & Identification

O157 Latex Agglutination

Rapid serotype confirmation

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STEC Toxin Detection

Shiga toxin immunoassay

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Sorbitol Solution

Biochemical testing

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API 20E Strips

Species identification

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Need O157:H7 Detection Support?

Our pathogen specialists can help optimize your EHEC detection and BSL-2 protocols

⚠️ For pathogen detection and laboratory use only. BSL-2 protocols required. Not for food, feed, household, cosmetic, therapeutic, or personal use.
AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia
Same-day dispatch • Australian stock • USDA-FSIS compliance documentation included

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