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EE Broth (Mossel) | Enterobacteriaceae Enrichment | Buffered BGB Glucose Broth | AS‑1216
$75.00 AUD
AuSaMicS Life Science • Culture Media
EE Broth (Mossel)
Enterobacteriaceae Enrichment • Buffered Brilliant Green Bile Glucose Broth
Selective enrichment broth formulated for the recovery and enrichment of Enterobacteriaceae from food, dairy, environmental, and other test samples. EE Broth (Mossel) combines glucose, bile salts, brilliant green, and phosphate buffering to support target organism growth while suppressing much of the accompanying Gram-positive and non-target flora.
Cat. No: AS-1216 Selective Enrichment Broth ✓ Australian Stock 🧫 Food Microbiology
🚀 Fast Dispatch: Australian supply 🧪 QC Ready: Microbiological quality 📋 Documentation: COA + SDS available
Enterobacteriaceae Workflow Medium
Selective Enrichment - supports target recovery
Bile + Brilliant Green - suppresses non-target flora
Buffered System - maintains broth performance
Food Testing Fit - routine QA workflows
Technical Support - method matching assistance
Mossel Enrichment Format
Designed for pre-plating enrichment prior to selective agar isolation
🔬 Product Overview
EE Broth (Mossel), also known as Enterobacteriaceae Enrichment Broth or Buffered Brilliant Green Bile Glucose Broth, is a selective enrichment medium widely used for the detection and recovery of members of the family Enterobacteriaceae from food and related samples.
The formulation supplies fermentable glucose and supportive nutrients, while bile salts and brilliant green inhibit many competing microorganisms. The phosphate buffering system helps stabilize medium performance during incubation, making it suitable for routine enrichment prior to subculture onto differential and selective plating media.
⚗️ Principle of the Medium
Sample inoculation
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Selective enrichment in EE Broth (Mossel)
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Recovery of Enterobacteriaceae with suppression of much competing flora
↓
Subculture to VRBG, MacConkey, EMB, or other selective/differential agar
↓
Selective enrichment in EE Broth (Mossel)
↓
Recovery of Enterobacteriaceae with suppression of much competing flora
↓
Subculture to VRBG, MacConkey, EMB, or other selective/differential agar
This broth is intended as an enrichment step rather than a final confirmatory medium. After incubation, enriched cultures are typically plated onto selective agars for isolation, colony observation, and subsequent biochemical or confirmatory testing.
🧬 Key Applications
🍽️ Food Microbiology
Used for enrichment of Enterobacteriaceae from foods, ingredients, dairy products, powders, processed items, and hygiene-related samples.
🏭 QA & Environmental Monitoring
Suitable for process hygiene programs, raw material screening, and environmental monitoring workflows where Enterobacteriaceae are used as indicator organisms.
🧪 Routine Enrichment Step
Functions as an intermediate enrichment stage before plating onto VRBG Agar, MacConkey Agar, EMB Agar, or other confirmatory media.
🔍 Indicator Organism Recovery
Helpful in workflows where detection of Enterobacteriaceae supports assessment of sanitary quality, process control, or product contamination risk.
✨ Key Features
- Selective enrichment broth for Enterobacteriaceae detection workflows
- Buffered formulation helps maintain stable medium performance
- Contains glucose as a fermentable carbohydrate source
- Bile salts and brilliant green enhance selectivity
- Compatible with routine food microbiology methods
- Suitable for downstream plating on selective/differential agar media
🧪 Typical Composition (g/L)
Final formulation may vary slightly by manufacturing standard and intended performance specification.
📊 Related Enterobacteriaceae Media Comparison
💡 Preparation & Use Guidance
Use Case:
For enrichment prior to selective plating and confirmation
For enrichment prior to selective plating and confirmation
Incubation:
Follow the validated method used in your laboratory workflow
Follow the validated method used in your laboratory workflow
Next Step:
Subculture onto suitable selective/differential agars
Subculture onto suitable selective/differential agars
Storage:
Store dehydrated medium tightly closed in a cool, dry place
Store dehydrated medium tightly closed in a cool, dry place
📋 Technical Specifications
| Product Name | EE Broth (Mossel) |
| Alternative Name | Enterobacteriaceae Enrichment / Buffered BGB Glucose Broth |
| Catalog Number | AS-1216 |
| Format | Dehydrated powder |
| Medium Type | Selective enrichment broth |
| Target Group | Enterobacteriaceae |
| Appearance | Beige to greenish-beige powder |
| Prepared Medium | Green to yellow-green broth |
| Storage | Keep tightly closed in a dry place, protected from light and moisture |
| Application | Food, dairy, QA, environmental and routine microbiology |
🎯 Typical Organism Scope
- ✓ Escherichia coli and related enterics
- ✓ Klebsiella, Enterobacter, Citrobacter
- ✓ Salmonella-associated screening workflows
- ✓ Indicator Enterobacteriaceae populations
✅ Quality & Documentation
- ✓ Microbiological grade raw materials
- ✓ Batch QC documentation
- ✓ COA available
- ✓ SDS support
📄 Available Documents
Certificate of Analysis (COA)
Safety Data Sheet (SDS)
Technical Data Sheet (TDS)
🧫 Related AuSaMicS Media
Need a matching Enterobacteriaceae workflow set?
AuSaMicS can support broth, plating media, supplements, and documentation for food and QC microbiology workflows.
For laboratory and research use only. Use according to validated laboratory methods and applicable quality standards.