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Egg Yolk Agar Base, Modified | Detection of Lecithinase & Lipase Activity | AS‑1217
$75.00 AUD
AuSaMicS Life Science • Culture Media
Egg Yolk Agar Base, Modified
Selective and differential medium base for lecithinase, lipase, and proteolytic activity studies
Premium dehydrated medium base designed for the preparation of egg-yolk supplemented diagnostic agar used in microbiology for the differentiation of organisms that produce lecithinase, lipase, and related extracellular enzymes. Commonly used in food, environmental, and clinical laboratories for selective isolation workflows and biochemical characterization.
Cat. No: AS-1217 Dehydrated Medium Base ✓ Australian Stock 🧫 Differential Medium
🚀 Fast Dispatch: Australian supply 🧪 Lab Grade: Microbiological quality 📋 Documents: COA + SDS available
Enzyme-Reaction Focus
Lecithinase Detection - opaque precipitation
Lipase Detection - pearly sheen / iridescence
Modified Base - flexible supplementing
Complete Support - technical documentation
Expert Assistance - media and method guidance
Egg Yolk Supplement Ready
Designed for use with sterile egg yolk emulsion and selective additives where required
🔬 Product Overview
Egg Yolk Agar Base, Modified is a dehydrated culture medium base used for preparing differential agar systems intended to demonstrate extracellular enzymatic reactions, especially lecithinase and lipase activity. After supplementation with sterile egg yolk emulsion, the medium helps visualize characteristic reactions such as opaque zones, precipitates, halo formation, and surface sheen around colonies.
It is particularly useful in workflows involving Clostridium spp., Bacillus spp., Staphylococcus spp., and other organisms where egg-yolk reactions provide valuable presumptive differentiation. The modified formulation offers a practical base for laboratories that require selective or customized egg-yolk media configurations.
⚗️ Detection Principle
Egg yolk phospholipids / lipids
↓
Lecithinase / Lipase-producing organism
↓
Opaque precipitate, halo, iridescent sheen, or pearly surface reaction
↓
Lecithinase / Lipase-producing organism
↓
Opaque precipitate, halo, iridescent sheen, or pearly surface reaction
Colonies may develop distinctive reactions depending on enzyme production and the final supplemented formulation. These visual effects aid presumptive differentiation and support confirmatory identification workflows.
🧬 Key Applications
🦠 Clostridium Differentiation
Useful in egg-yolk reaction studies for presumptive differentiation of lecithinase-positive anaerobes in supplemented selective systems.
🍽️ Food Microbiology
Applied in food testing laboratories for isolation and differentiation of enzyme-producing organisms from raw materials and finished products.
🏥 Clinical & Research Use
Suitable for microbiology laboratories performing biochemical characterization, culture differentiation, and strain comparison studies.
🧪 Custom Media Preparation
Flexible base for laboratories preparing modified egg-yolk media with added supplements, antibiotics, or selective agents.
✨ Key Features
- Modified egg-yolk agar base for flexible laboratory use
- Supports visual differentiation of lecithinase and lipase reactions
- Suitable for selective supplementation where required by method
- Useful in anaerobic and aerobic workflows depending on target organism
- Clear reaction backgrounds for colony interpretation
- Available with COA and SDS support for procurement and QC needs
📊 Related Media Comparison
💡 Preparation & Use Guidance
Supplementation:
Use with sterile egg yolk emulsion as required by the method
Use with sterile egg yolk emulsion as required by the method
Selective Additives:
May be added depending on target organism and protocol
May be added depending on target organism and protocol
Interpretation:
Read colony morphology and surrounding reaction zones carefully
Read colony morphology and surrounding reaction zones carefully
Storage:
Store dehydrated medium tightly closed in a cool, dry place
Store dehydrated medium tightly closed in a cool, dry place
📋 Technical Specifications
| Product Name | Egg Yolk Agar Base, Modified |
| Catalog Number | AS-1217 |
| Format | Dehydrated powder |
| Application | Selective / differential microbiological medium base |
| Primary Use | Lecithinase and lipase reaction studies |
| Supplement Required | Egg yolk emulsion |
| Appearance | Light beige to pale yellow powder |
| Prepared Medium | Opaque to slightly turbid after supplementation |
| Storage | Keep tightly closed in a dry place, protected from moisture |
| Compliance | For laboratory and research use |
🎯 Typical Use Targets
- ✓ Clostridium spp.
- ✓ Bacillus spp.
- ✓ Staphylococcus spp.
- ✓ Other enzyme-producing isolates
✅ Quality Standards
- ✓ Microbiological grade raw materials
- ✓ Batch-level QC documentation
- ✓ COA available on request / supply
- ✓ SDS support for procurement compliance
📄 Documentation
Certificate of Analysis (COA)
Safety Data Sheet (SDS)
Technical Data Sheet (TDS)
🧫 Related AuSaMicS Microbiology Products
Need a more selective version or matching supplement set?
AuSaMicS can support QC, documentation, and related media recommendations for your microbiology workflow.
For laboratory and research use only. Final formulation, supplements, and performance characteristics may vary depending on the target method and intended application.