Fletcher Medium Base | Selective Medium for Leptospira Cultivation | AS‑1231
$155.00 AUD
AuSaMicS Life Science • Culture Media
Fletcher Medium Base
Semisolid Leptospira Medium Base
Fletcher Medium Base is a classic semisolid enrichment and maintenance medium for Leptospira species, used for the isolation, cultivation, and observation of these fastidious spirochaetes from clinical, veterinary, and environmental specimens. Its low agar concentration supports characteristic subsurface growth and facilitates motility observation.
AS-1231
Leptospira Culture
✓ Semisolid Base
Veterinary & Clinical Use
🧪 Low-Agar Semisolid Format
🔬 Supports Motility Observation
❄️ Typically Supplemented with Serum
🏆 Leptospira Recovery Specialist
🌀 Designed for Leptospira spp.
🧫 Semisolid growth format
📋 COA + SDS available
⚡ Suitable for routine preparation
🐄 Useful in veterinary diagnostics
Classical Fletcher System
For isolation, maintenance, and observation
🔬 Principle of the Medium
Fletcher Medium Base provides a nutritionally supportive semisolid environment for Leptospira, organisms that are slow-growing, delicate, and difficult to recover using ordinary bacteriological media.
Peptones and beef extract contribute essential nutrients, while the low agar concentration produces a soft semisolid matrix that assists in detecting characteristic subsurface growth and motility. The base is typically enriched with sterile serum before use.
Nutrient base + low agar + serum enrichment
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Supports Leptospira survival, growth, and motility observation
↓
Supports Leptospira survival, growth, and motility observation
🧬 Applications
🏥 Clinical Isolation
Used for recovery of Leptospira from blood and other clinical specimens where delicate handling and prolonged incubation are required.
🐄 Veterinary Diagnostics
Suitable for leptospiral investigations in veterinary samples, including kidney and urine-associated materials.
🔬 Maintenance & Observation
Appropriate for maintaining cultures and assessing characteristic leptospiral growth in semisolid medium.
🌿 Environmental & Research Use
Useful in leptospiral ecology, strain propagation, and laboratory research workflows requiring classical culture systems.
📊 Comparative Leptospira Media Guide
💡 Preparation & Use Notes
Medium Type:
Semisolid dehydrated base
Semisolid dehydrated base
Supplementation:
Typically enriched with sterile serum before use
Typically enriched with sterile serum before use
Incubation:
Usually around 30–32°C with prolonged observation
Usually around 30–32°C with prolonged observation
Observation:
Dark-field examination may be used for motility assessment
Dark-field examination may be used for motility assessment
📋 Technical Specifications
| Product Name | Fletcher Medium Base |
| Catalog Number | AS-1231 |
| Format | Dehydrated powder |
| Medium Type | Semisolid leptospiral medium base |
| Typical pH | 7.9 ± 0.2 |
| Prepared Appearance | Very light yellow, slightly opalescent semisolid gel |
| Storage | Store dry at 10–30°C in tightly closed container |
| HS Code | 3821.00.00 |
🧫 Target Organism Profile
- ✓ Designed for Leptospira spp.
- ✓ Suitable for delicate, slow-growing spirochaetes
- ✓ Useful in routine and research culture work
- ✓ Supports classical leptospiral workflows
✅ Quality Standards
- ✓ Controlled semisolid consistency
- ✓ Batch-to-batch reproducibility
- ✓ COA issued per lot
- ✓ Suitable for institutional procurement
Related Leptospira Culture Options
Disclaimer: For laboratory, veterinary, and research use only. Not for direct therapeutic or household use.