GN Broth (Gram-Negative Broth) | Selective Enrichment Media | Ausamics

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Gram Negative Broth, GN Broth

AuSaMicS Life Science™ | www.ausamics.com.au

Product Description

Cat. No. AS-1244

For Laboratory Use Only

Catalog Number

AS-1244

Medium Type

Selective Enrichment Broth

Target Organisms

Salmonella & Shigella

Storage

15–30 °C (powder)

Description

GN Broth is a selective enrichment medium used for the isolation and recovery of Shigella and Salmonella from a wide variety of sample types. Peptone serves as the principal source of nutrients, while sodium deoxycholate and sodium citrate suppress the growth of many Gram-positive organisms and certain competing Gram-negative bacteria.

Phosphate buffering helps maintain pH stability during incubation, supporting consistent enrichment performance. Recovery of target pathogens is enhanced when mannitol is used at a higher concentration instead of dextrose, as this helps inhibit Proteus growth. For improved isolation and confirmation, GN Broth is commonly used together with selective and non-selective agar media.

Key Features & Benefits

🎯

Selective Enrichment Suppresses unwanted microbial growth to support recovery of target enteric pathogens.

🧪

Pathogen Recovery Support Formulated to enhance isolation of Shigella and Salmonella from mixed samples.

🌍

Versatile Applications Suitable for clinical diagnostics, food safety testing, water microbiology, and hygiene monitoring.

📏

Standards-Oriented Performance Designed for reliable enrichment workflows used alongside selective and non-selective agar media.

Applications

🏥

Clinical Diagnostics

Selective enrichment of enteric pathogens from clinical specimens
Support medium for isolation workflows targeting Salmonella and Shigella

🍽️

Food Safety

Recovery of target pathogens from food and environmental monitoring samples
Useful in routine quality control and contamination investigations

💧

Water Testing

Selective enrichment of Gram-negative enteric bacteria from water samples
Applicable in hygiene, sanitation, and microbiological surveillance programs

Composition & Properties

PeptonePrimary nutrient source for bacterial growth Sodium DeoxycholateSelective agent to inhibit unwanted organisms Sodium CitrateSelective inhibitor for competing flora Phosphate BufferMaintains pH stability during incubation Carbohydrate SourceMannitol preferred over dextrose for improved inhibition of Proteus growth Medium FunctionSelective enrichment of Shigella and Salmonella

Preparation

Step	Procedure
1	Suspend the recommended quantity of dehydrated medium in purified water according to the approved formulation or label instructions.
2	Heat with gentle agitation until the medium is completely dissolved.
3	Dispense into suitable sterile containers or tubes for enrichment use.
4	Sterilize using validated laboratory procedures appropriate for the medium formulation.
5	Cool to the recommended inoculation temperature before use.

Note For best isolation performance, GN Broth is commonly used in conjunction with both selective and non-selective agar media.

Incubation & Interpretation

UseSelective enrichment prior to plating on appropriate agar media Target RecoverySupports enrichment of Salmonella and Shigella while suppressing competing flora InterpretationFollowing enrichment, subculture onto selective and/or non-selective agar for isolation and confirmation of suspect colonies

Storage

Powdered MediumKeep the container at 15–30 °C in a dry place and tightly sealed Prepared MediumStore at 2–8 °C HandlingProtect from moisture and contamination during storage and preparation

⚠ Warning For laboratory use only. Not intended for human, animal, or food consumption.

Disclaimer This product is supplied for laboratory and industrial microbiology use only. Performance depends on correct preparation, storage, inoculation, incubation, and downstream confirmation procedures. End users are responsible for validating suitability for their intended application and quality system.

Safety Data Sheet

Gram Negative Broth, GN Broth | Cat. AS-1244

SDS — GHS Format

SDS-AS-1244 | Rev. 1.0

Issue Date: March 2026

Section 1 — Identification

Product NameGram Negative Broth, GN Broth Catalog No.AS-1244 Recommended UseSelective enrichment of Shigella and Salmonella in laboratory and industrial microbiology SupplierAuSaMicS Pty Ltd, 31 Longview CT, Thomastown VIC 3074, Australia Emergency Contact+61 412 520 598 | support@ausamics.com.au Emergency ServicesAustralia: 000 | Poisons Information Centre: 13 11 26

Section 2 — Hazard Identification

GHS Classification: Not generally classified as hazardous under normal laboratory use. Powder may cause mild irritation to eyes, skin, or respiratory tract if dust is generated during handling.

Signal WordNone required Hazard StatementsNot classified as hazardous. Avoid unnecessary dust formation and direct contact with eyes, skin, and respiratory tract. Precautionary StatementsUse good laboratory practice. Avoid inhalation of dust. Wear suitable gloves, laboratory coat, and eye protection when handling bulk powder. Regulatory StatusFor laboratory use only. Not intended for human, animal, or food consumption.

Section 3 — Composition / Ingredients

Component	Typical Role	Function
Peptone	Nutrient base	Supports bacterial enrichment
Sodium Deoxycholate	Selective agent	Inhibits unwanted organisms
Sodium Citrate	Selective agent	Suppresses competing flora
Phosphate Buffer	Buffer system	Maintains pH stability
Mannitol / Carbohydrate source	Fermentable substrate	Supports target recovery while helping reduce Proteus overgrowth

Section 4 — First Aid Measures

Skin ContactWash with soap and water. Seek medical advice if irritation develops. Eye ContactRinse cautiously with water for several minutes. Seek medical advice if irritation persists. InhalationMove to fresh air if dust is inhaled. Seek medical advice if symptoms persist. IngestionRinse mouth with water. Seek medical advice if discomfort occurs or large quantities are ingested.

Section 5 — Fire-Fighting Measures

Suitable Extinguishing MediaWater spray, foam, dry chemical, or carbon dioxide Specific HazardsCombustible organic components may generate smoke and carbon oxides during fire conditions Protective EquipmentUse standard fire-fighting PPE and respiratory protection as needed

Section 6 — Accidental Release Measures

Personal PrecautionsAvoid generating dust. Wear gloves and eye protection during cleanup. Environmental PrecautionsPrevent bulk release to drains and waterways. Clean-Up MethodsSweep or vacuum into a suitable container. Wipe remaining residue with a damp cloth.

Sections 7–16 — Handling, Storage, PPE, Physical, Stability, Disposal, Transport, Regulatory

HandlingUse good laboratory practices. Avoid inhalation of dust and unnecessary contact with eyes and skin. StorageStore dehydrated medium at 15–30 °C in a dry, tightly sealed container. Store prepared medium at 2–8 °C. PPEGloves, safety glasses, and laboratory coat recommended for bulk handling. AppearanceDehydrated powder for microbiological medium preparation StabilityStable under recommended storage conditions DisposalDispose according to local laboratory and environmental regulations TransportTypically not classified as dangerous goods Prepared ByAuSaMicS Pty Ltd — Quality & Regulatory Affairs

Disclaimer This SDS-style content is provided in website format and should be aligned with your approved internal or regulatory safety documentation before publication where required.

Certificate of Analysis

Gram Negative Broth, GN Broth | Cat. AS-1244

COA — Batch Specific

COA-AS-1244-L001

Issue Date: March 2026

Product Information

Product NameGram Negative Broth, GN Broth Catalog No.AS-1244 Medium TypeSelective enrichment broth Target OrganismsSalmonella and Shigella ApplicationSelective enrichment for clinical, food, and water microbiology workflows Storage15–30 °C, dry and tightly sealed

Lot / Batch Information

Lot NumberAS1244-L001 Manufacture DateMarch 2026 Expiry DateMarch 2029 AppearanceConforms ✓ Pack SizeTBD Prepared Medium Storage2–8 °C

Analytical Test Results

No.	Test / Criteria	Method	Specification / Limit	Result	Status
1	Appearance	Visual inspection	Homogeneous dehydrated powder	Conforms	PASS
2	Colour of Medium	Visual inspection	Conforms to standard	Conforms	PASS
3	Solubility / Reconstitution	Preparation test	Forms suitable enrichment medium on preparation	Conforms	PASS
4	pH (prepared medium)	Potentiometry	Conforms to approved specification	Conforms	PASS
5	Selectivity	Biological performance test	Inhibits unwanted organisms as specified	Conforms	PASS
6	Growth Promotion	Biological performance test	Supports recovery of representative target organisms	Conforms	PASS
7	Sterility Check	Incubation / QC	No contamination in uninoculated control	Conforms	PASS

Overall Batch Disposition

RELEASED — COMPLIES WITH SPECIFICATIONS

Authorisation

Analysed By

QC Analyst | AuSaMicS Pty Ltd

Date: _______________

Approved & Released By

Quality Manager | AuSaMicS Pty Ltd