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ISP Medium 1 (AS-1210) | Tryptone-Yeast Extract Broth for Streptomyces
$115.00 AUD
AuSaMicS Life Science • ISP Actinomycete Media
ISP Medium 1
Tryptone Yeast Extract Broth for Streptomyces Enrichment & Inoculum Preparation
A simple but highly useful broth medium from the International Streptomyces Project used for inoculum preparation, enrichment, and physiological support of Streptomyces species and related actinomycetes.
AS-1251 🧫 ISP Series ✓ Streptomyces Inoculum 🧪 Taxonomy Support
Type: BrothPreparation: 8.0 g/LpH: 7.0 ± 0.2
Inoculum Specialist
Tryptone — peptides and nitrogen
Yeast Extract — vitamins and trace nutrients
Simple Formula — reproducible inoculum base
ISP Workflow — links to ISP 2 and ISP 4 plating
Overview
ISP Medium 1, also known as Tryptone Yeast Extract Broth, is a basal broth from the International Streptomyces Project used primarily for preparation of inoculum and enrichment of Streptomyces species. Its simple composition promotes reproducible early growth without imposing overly complex nutritional variables. citeturn584042search0turn584042search7
Because morphology and pigmentation studies on ISP agar media depend heavily on consistent inoculum quality, ISP 1 functions as the practical starting point in many taxonomic and characterization workflows. ATCC strain handling information also links Streptomyces cultivation to ISP Medium 1 followed by ISP Medium 2 agar. citeturn421530search1turn421530search11
This makes ISP 1 less of a final differentiation medium and more of a controlled preparatory broth within the wider ISP system.
Typical Composition (per Liter)
| Ingredient | Concentration | Role |
|---|---|---|
| Tryptone | 5.0 g | Peptides, amino acids, nitrogen source |
| Yeast extract | 3.0 g | Vitamin B complex, trace factors, growth support |
Final pH: 7.0 ± 0.2 at 25°C. Typical preparation: 8.0 g/L. citeturn584042search0turn584042search7
Applications
- Preparation of Streptomyces inoculum for ISP agar media
- General enrichment and cultivation of actinomycetes
- Physiological studies including carbohydrate-use variants when supplemented
- Pre-culture before morphology and pigmentation evaluation on ISP 2/3/4
ISP Workflow Comparison
| Medium | Role | Best Use |
|---|---|---|
| ISP 1 | Broth inoculum medium | Pre-growth before plating and characterization |
| ISP 2 | Yeast-malt agar | Morphology, aerial mycelium, pigment expression |
| ISP 3 | Oatmeal agar | Sporulation and colony character comparison |
| ISP 4 | Inorganic salts starch agar | Starch utilization and characterization |
Technical Specifications
| Cat. No. | AS-1251 |
| Medium Type | Broth |
| Preparation | 8.0 g/L |
| Final pH | 7.0 ± 0.2 |
| Sterilization | 121°C for 15 minutes |
| Typical Use | Streptomyces inoculum and enrichment |
Preparation Protocol
- 1Suspend 8.0 g in 1 L purified water.
- 2Heat if necessary until dissolved.
- 3Dispense into tubes or flasks.
- 4Autoclave at 121°C for 15 minutes. citeturn584042search0turn584042search7
Pack Sizes & Suggested Pricing
| 100 g | AUD 28 |
| 250 g | AUD 56 |
| 500 g | AUD 92 |
| 1 kg | AUD 155 |
| 5 kg | AUD 590 |
AuSaMicS Compliance & Supply Notes
Supplied for laboratory and research workflows. Batch-specific documentation may include Certificate of Analysis, Technical Data Sheet, and Safety Data Sheet depending on customer requirements.
For laboratory and research use only. Not for household, therapeutic, or pharmaceutical applications.
AuSaMicS Pty Ltd
31 Longview CT, Thomastown, VIC 3074, Australia
ABN 56676640467
www.ausamics.com.au
support@ausamics.com
+61 412 520 598