ISP Medium 3 (AS-1212) | Oatmeal Agar for Streptomyces Characterization
ISP Medium 3
Oatmeal Agar for Streptomyces Sporulation & Colony Characterization
A classical oatmeal-based agar medium from the ISP system, used for examining colony character, sporulation behavior, and taxonomic differentiation of Streptomyces species.
Differentiation Specialist
Overview
ISP Medium 3, commonly known as Oatmeal Agar, is a standard International Streptomyces Project medium used for cultivation and characterization of Streptomyces species. Oatmeal provides a relatively gentle, natural nutrient base that often encourages characteristic colony morphology and sporulation patterns useful for taxonomic comparison. citeturn944494search0turn584042search1
Compared with richer synthetic or semi-synthetic media, ISP 3 often reveals differences in aerial growth, spore mass, and colony surface that may not be as obvious elsewhere, making it valuable in comparative actinomycete studies.
Within an ISP characterization set, ISP 3 is typically interpreted alongside ISP 2 and ISP 4 to build a fuller phenotypic picture.
Typical Composition (per Liter)
| Ingredient | Concentration | Role |
|---|---|---|
| Oat meal | 20.0 g | Complex carbon and nutrient source |
| Agar | 18.0 g | Solidifying agent |
| Ferric sulphate heptahydrate | 0.001 g | Trace element support |
| Manganese chloride tetrahydrate | 0.001 g | Trace element support |
| Zinc sulphate heptahydrate | 0.001 g | Trace element support |
Final pH commonly reported around 7.3 ± 0.2. citeturn944494search0turn584042search1
Applications
- Streptomyces taxonomy and morphological comparison
- Sporulation and aerial growth evaluation
- Colony color and surface character assessment
- Comparative actinomycete studies within ISP workflows
Technical Specifications
| Cat. No. | AS-1253 |
| Medium Type | Agar |
| Preparation | ~38.0 g/L |
| Final pH | 7.3 ± 0.2 |
| Sterilization | 121°C for 15 minutes |
| Primary Use | Morphology and sporulation studies |
Preparation Protocol
- 1Suspend the required amount in purified water.
- 2Heat to dissolve the medium completely.
- 3Autoclave at 121°C for 15 minutes.
- 4Cool and pour plates. citeturn944494search0turn584042search1
Pack Sizes & Suggested Pricing
| 100 g | AUD 39 |
| 250 g | AUD 82 |
| 500 g | AUD 138 |
| 1 kg | AUD 235 |
| 5 kg | AUD 910 |
AuSaMicS Compliance & Supply Notes
Supplied for laboratory and research workflows. Batch-specific documentation may include Certificate of Analysis, Technical Data Sheet, and Safety Data Sheet depending on customer requirements.
For laboratory and research use only. Not for household, therapeutic, or pharmaceutical applications.