ISP Medium 4 (AS-1213) | Inorganic Salts Starch Agar (ISP4)
ISP Medium 4
Inorganic Salts Starch Agar for Streptomyces Characterization & Starch Utilization
A defined starch-based actinomycete medium from the ISP series used for characterization of Streptomyces species, especially where starch utilization and more controlled nutritional conditions are important.
Biochemical Differentiation Specialist
Overview
ISP Medium 4, Inorganic Salts Starch Agar, is formulated for characterization of Streptomyces species under more controlled nutrient conditions than richer actinomycete media. The starch base and mineral composition make it particularly useful in assessing growth character and starch utilization-related differentiation. citeturn584042search2turn584042search3
Within ISP workflows, it commonly complements ISP 2 and ISP 3 by providing a more defined medium where nutrient abundance is lower and metabolic character can be observed more clearly. Technical sheets explicitly recommend it for characterization of Streptomyces species. citeturn584042search2turn584042search3
For laboratories working with actinomycete taxonomy, ISP 4 is one of the most informative media for biochemical comparison within the ISP set.
Typical Composition (per Liter)
| Ingredient | Concentration | Role |
|---|---|---|
| Soluble starch | 10.0 g | Principal carbon source |
| Dipotassium hydrogen phosphate | 1.0 g | Buffer component |
| Magnesium sulphate | 1.0 g | Mineral support |
| Sodium chloride | 1.0 g | Osmotic balance |
| Ammonium sulphate | 2.0 g | Nitrogen source |
| Calcium carbonate | 2.0 g | pH moderation and growth support |
| Trace salts | q.s. | Micronutrient support |
| Agar | 18.0–20.0 g | Solidifying agent |
Technical sheets cite soluble starch 10 g/L with salts-based formulation and pH around 7.2. citeturn584042search2turn584042search3
Applications
- Streptomyces characterization under defined nutrient conditions
- Starch hydrolysis observation and related differentiation
- Comparative taxonomy within ISP media sets
- Actinomycete physiological studies
Technical Specifications
| Cat. No. | AS-1254 |
| Medium Type | Agar |
| Preparation | ~35–36 g/L |
| Final pH | 7.2 ± 0.2 |
| Sterilization | 121°C for 15 minutes |
| Primary Use | Starch-based characterization |
Preparation Protocol
- 1Suspend the required amount in purified water.
- 2Heat to dissolve completely.
- 3Autoclave at 121°C for 15 minutes.
- 4Cool and pour plates. citeturn584042search2turn584042search3
Pack Sizes & Suggested Pricing
| 100 g | AUD 36 |
| 250 g | AUD 78 |
| 500 g | AUD 132 |
| 1 kg | AUD 225 |
| 5 kg | AUD 875 |
AuSaMicS Compliance & Supply Notes
Supplied for laboratory and research workflows. Batch-specific documentation may include Certificate of Analysis, Technical Data Sheet, and Safety Data Sheet depending on customer requirements.
For laboratory and research use only. Not for household, therapeutic, or pharmaceutical applications.